Project description:The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria 4 samples were analyzed which included 3 biological replicate, 1 technical replicate and 1 dye swap
Project description:The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria 4 samples were analyzed which included 3 biological replicate, 1 technical replicate and 1 dye swap
Project description:The transcriptome of Actinobacillus pleuropneumoniae 4h static biofilms was compared to the transcriptome of 6h static biofilm 4 samples were analyzed which included 3 biological replicate, 1 technical replicate and 1 dye swap
Project description:The transcriptome of Actinobacillus pleuropneumoniae dripbiofilm biofilms was compared to the transcriptome of effluent cells 4 samples were analyzed which included 3 biological replicate, 1 technical replicate and 1 dye swap
Project description:To reveal the transcriptional profiles of Actinobacillus pleuropneumoniae under biofilm and planktonic growth, we established a biofilm-forming culture method and constructed a mutant strain Δpga with defect in biofilm formation. Wild-type and Δpga mutant strains of Actinobacillus pleuropneumoniae strain 4074 were cultured in bottles with shaking for planktonic (WT_PK) and in microplates in static status for biofilm (WT_BF, Δpga), respectively. The bacteria in logarithmic growth period of different culture groups were collected for RNA seq.
Project description:To elucidate the mechanisms by which ammonia induced the dispersion and proliferation of A. pleuropneumoniae biofilms, NH3·H2O was added to biofilms and planktonic bacteria cultured to the log-phase for short-term stimulation and incubation. Subsequently, planktonic bacteria after ammonia stimulation (PK_NH3, the rapidly proliferating planktonic bacteria after ammonia stimulation), bacteria in the biofilm adhered layer (BF_NH3, adhered biofilm) and the supernatant layer (Up_NH3, bacteria dispersed from the BF layer ) were collected, along with the supernatant layer (Up_DspB) and adhered layer (BF_DspB) treated with DspB, and untreated control groups (PK_Con, Up_Con, BF_Con). RNA was extracted for transcriptome sequencing analysis.
Project description:Actinobacillus pleuropneumoniae is the etiologic agent of contagious pleuropneumonia, an economically important disease of commercially reared swine throughout the world. To cause this disease, A. pleuropneumoniae must rapidly overcome porcine pulmonary innate immune defenses. Effects of koromycin, an antimicrobial agent that acts as an noncompetitive inhibitor of the interaction of NQR with its quinone substrate, on the transcriptome of A. pleuropneumoniae was investigated.