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GATA factors efficiently direct cardiac fate from ESCs

ABSTRACT: Gata5 efficiently promotes cardiomyocyte fate from murine ESCs.By removing serum from the culture conditions, GATA4 and GATA6 are each also able to efficiently promote cardiogenesis in ESC derivatives, with some distinctions. Thus, GATA factors can function in ESC derivatives upstream of other cardiac transcription factors to direct specification of progenitors with cardiac potential. We used microarray to compared the global gene expression of Gata5-induced cardiac cells with other growth factor directed ESC-derived cardiac cells

ORGANISM(S): Mus musculus

PROVIDER: GSE43831 | GEO | 2013/02/02

SECONDARY ACCESSION(S): PRJNA188325

REPOSITORIES: GEO

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GATA factors efficiently direct cardiac fate from ESCs

Project description:Gata5 efficiently promotes cardiomyocyte fate from murine ESCs.By removing serum from the culture conditions, GATA4 and GATA6 are each also able to efficiently promote cardiogenesis in ESC derivatives, with some distinctions. Thus, GATA factors can function in ESC derivatives upstream of other cardiac transcription factors to direct specification of progenitors with cardiac potential. We used microarray to compared the global gene expression of Gata5-induced cardiac cells with other growth factor directed ESC-derived cardiac cells iGata5 ESCs were induced with doxycycline at day 4 and harvested at day16 for RNA extraction and hybridization on Affymetrix microarrays.

2013-02-02 | E-GEOD-43831 | biostudies-arrayexpress

Tmem88a Mediates GATA-dependent Specification of Cardiomyocyte Progenitors by Restricting Canonical WNT Signaling

2013-10-21 | GSE44026 | GEO

Tmem88a Mediates GATA-dependent Specification of Cardiomyocyte Progenitors by Restricting Canonical WNT Signaling

Project description:We sequenced mRNA from zebrafish wild-type embryos, gata5 morphants, gata6 morphants, and gata5/6 morphants at bud and 6-somite developmental stages to identify genes co-operatively regulated by gata5 and gata6 during cardiomyocyte progenitor specification. Samples were collected in duplicate, with 40 embryos per sample. Single 36-base pair reads were sequenced on the Illumina Genome Analyzer IIx

2013-10-21 | E-GEOD-44026 | biostudies-arrayexpress

Examining the immediate early targets of Gata4 and Gata5.

2008-07-15 | GSE8496 | GEO

Transcription profiling of Xenopus animal cap cardiogenesis model to examine immediate early targets of Gata4 and Gata5.

Project description:Gata factors are amongst the genes expressed early on in the process of cardiogenesis. We used microarrays to examine the immediate early targets of Gata4 and Gata5 in the Xenopus leavis animal cap cardiogenesis model. We hope to use these data to examine the roles of Gata4 and Gata5 in cardiogenesis and also to begin to dissect out the common and distinct targets of Gata4 and Gata5. Experiment Overall Design: Xenopus leavis embryos were injected at the one cell stage with dexamethasone-inducible Gata4 or Gata5 mRNA constructs (to examine endodermal and mesodermal targets) or with Gata4 in the presence of Dkk1 (to examine mesodermal targets.) Embryos were cultured to stage 9, whereupon animal caps were excised and cultured for 2.5 hours in media containing dexamethasone to induce the constructs and cycloheximide to block de novo protein synthesis and thus give only immediate early targets.

2008-10-18 | E-GEOD-8496 | biostudies-arrayexpress

ATAC-seq of gata5GFP+/- cells in WT and Gata5/6 deficient zebrafish embryos during gastrulation

Project description:Gata5/6 are essential regulators in zebrafish heart development. To understand how Gata5/6 affects the chromatin accessibility and regulates cardiac gene expression, we FACS-isolated GFP+ and GFP- cells from Tg(gata5:GFP) transgenic embryos in both control and Gata5/6 deficient embryos (injected with Gata5/6 morpholinos) and conducted ATAC-seq. We performed the experiments at 8 hours post fertilization, intending to dissect the role of Gata5/6 in the earliest step in cardiac lineage specification. We did 2-3 biological replicates for each sample. ATAC-seq libraries were made using previously published protocol (Buenrostro et al., Nat. Methods, 2013. DOI: 10.1038/nmeth.2688) and sequenced on Illumina Hiseq2500.

2021-12-31 | E-MTAB-10427 | biostudies-arrayexpress

Population RNA-seq of human ESC-derived cardiac derivatives

Project description:Population RNA-seq of human ESC-derived cardiac derivatives

| PRJEB30993 | ENA

Population RNA-seq of human ESC-derived cardiac derivatives

Project description:In vitro cardiac differentiation of human ESCs recapitulates in vivo embryonic heart development, and thus, serves as an excellent tool to investigate human cardiac development. Identification of molecular signatures during cardiac differentiation of human ESCs is instrumental for advancing our understanding of human cardiogenesis. We, as well as others have improved cardiac differentiation protocols significantly in recent years; however, detailed molecular mechanisms involved in cardiac lineage commitment have not yet been clearly defined. Based on this, we tried to identify the cellular hierarchies and molecular signatures of each of the in vitro human ESC-differentiating cardiac cell lineages through the well-established cardiac differentiatio protocol by Wnt signaling modulation and the FACS-sorted population RNA-seq analyses.

2019-03-31 | E-MTAB-7537 | biostudies-arrayexpress

Early Multipotent Cardiovascular Progenitors Derived from Pluripotent Stem Cells Engraft in Postmyocard Infarted Primate

Project description:During embryogenesis, the cardiac cell fate is acquired as early as gastrulation. There is compelling evidence that embryonic stem cells (ESC) can recapitulate early steps in cardiogenesis. Identification from human pluripotent stem cells of early cardiovascular cell progenitors, at the origin of the first cardiac lineage, would shed light on human normal and pathological cardiogenesis and would pave the way toward cell therapy for cardiac degenerative diseases. Here, we report the isolation, and a phenotypic characterisation of an early Oct-4+, SSEA-1+, Mesp1+ population of cardiovascular progenitors derived from human pluripotent stem cells. This multipotential progenitor features the capability to generate cardiomyocytes as well as smooth muscle and endothelial cells. We further bring a proof of concept that these progenitors can be used in cardiac regenerative medicine as allografted in infarcted non human primate myocardium, they differentiate in ventricular myocytes without any adverse effect. One RNA sample from HUESC-POU5F1 was compared in dye-swap to undifferentiated HUESC. Three distinct RNA samples from BMP2-induced SSEA1+ cells were compared in dye-swap to paired remaining SSEA1-. Reference samples correspond to undifferentiated HUESC and to SSEA1 negative cells, respectively. population respectivement.

2011-02-08 | E-GEOD-20230 | biostudies-arrayexpress

LncRNA expression profiling of the early mesoderm reveals LncCPR as a novel regulator of cardiogenesis in mice

Project description:Whole mount in situ hybridization analysis of the 17 lncRNAs revealed that lncRNAs 2410006H16Rik and 4930500J02Rik were expressed in mouse hearts at E9.5. 4930500J02Rik, renamed Long Noncoding Cardiac-promoting RNA (LncCPR), was also enriched in postnatal and adult heart tissue. We hypothesized that the loss of LncCPR expression would directly affect cardiogenesis. To test this hypothesis, we performed functional studies of ESC differentiation after knocking out the LncCPR gene with the CRISPR/Cas9 system. We profiled the transcriptome of mouse ES cells after the loss of LncCPR expression. We found the downregulation of Mesp1 and cardiac transcription factors, as well as a significant reduction in contractility and levels of cardiac structural protein TNNT2. Our findings indicate that LncCPR is a novel, specific regulator of Mesp1, and therefore, of early cardiogenesis.

2019-03-21 | GSE79499 | GEO

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