Enhancer Transcripts Mark Active Estrogen Receptor Binding Sites

ABSTRACT: In this study, we used Global Run-On sequencing (GRO-seq), a method that assays the genome-wide location and orientation of all active RNA polymerases. We generated a global profile of active transcription at ERα binding sites in MCF-7 human breast cancer cells in response to short time course of E2 treatment. This method enabled us to detect active transcription at enhancers and define a class of primary transcripts transcribed uni- or bidirectionally from the ERα binding sites. The raw data used in this study is from GSE27463 but sequenced to a greater depth.

ORGANISM(S): Homo sapiens

PROVIDER: GSE43835 | GEO | 2013/05/08

SECONDARY ACCESSION(S): PRJNA187528

REPOSITORIES: GEO