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DNA methylation dynamics during development in the rice blast fungus [Bisulfite-Seq]

ABSTRACT: Cytosine methylation is an important epigenetic modification of DNA that is involved in genome defense and transcriptional regulation in eukaryotes. Despite extensive efforts to understand genome-wide distribution and function of DNA methylation in mammals and plants, contribution of DNA methylation to biology of microbial eukaryotes is largely unknown to date. Here we used genetic manipulations and high-throughput bisulphite sequencing on the model plant pathogenic fungus, Magnaporthe oryzae to elucidate the dynamics and mechanics of DNA methylation during pathogenic development.

ORGANISM(S): Pyricularia oryzae KJ201

PROVIDER: GSE43844 | GEO | 2016/01/29

SECONDARY ACCESSION(S): PRJNA187573

REPOSITORIES: GEO

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DNA methylation dynamics during development in the rice blast fungus [RNA-Seq]

Project description:Cytosine methylation is an important epigenetic modification of DNA that is involved in genome defense and transcriptional regulation in eukaryotes. Contribution of DNA methylation to biology of microbial eukaryotes is largely unknown to date. Here we used RNA-seq to examine the impact of DNA methylation on transcriptional output in the genome of a model pathogenic fungus, Magnaporthe oryzae by comparing expression profiles of wild-type and methylation-deficient mutant strains.

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