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Genome-wide analysis of LINK1 and LINK2 effects on the Arabidopsis transcriptome

ABSTRACT: The goal of this study was to compare the transcriptional profile (RNA-seq) of wild type plants with that of link1link2 double mutants grown under continous light or long day (16h light/8 h darkness) conditions.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE43865 | GEO | 2013/08/02

SECONDARY ACCESSION(S): PRJNA188075

REPOSITORIES: GEO

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Genome-wide analysis of LINK1 and LINK2 effects on the Arabidopsis transcriptome

Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of wild type plants with that of link1link2 double mutants grown under continous light or long day (16h light/8 h darkness) conditions. In the first experiment wild-type and link1link2 mutant plants were grown for two weeks under continuous white light conditions at 22 degrees centigrades and the transcriptional profile of these plants was analyzed using RNA-seq. In the second experiment, wild-type and link1link2 mutants were grown under long day conditions (16 h light/8 h darkness), and the transcriptional profile of these plants was analyzed in samples harvested every 4 hours over a 24 hour time-course, starting 2 hours after the onset of the light period.

2013-08-02 | E-GEOD-43865 | biostudies-arrayexpress

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Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of lsm4 and lsm5 Arabidopsis mutants with their respective wild type plants grown under continous light conditions

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Project description:We performed a tranascriptomic analysis of Ler, ebs and shl-2 plants in order to reveal targets whose transcription is regulated by EBS and SHL. The analysis was performed in WT plants (Ler), as well as mutants plants in which the expression of EBS and SHL was previously shown to be null, namely ebs and shl-2 mutant. To perform this study, plants were grown for 18 days under short days (SD) conditions (8h light, 16h darkness) in MS solid media. Three independent biological samples were hybridized separately.

2014-10-14 | E-GEOD-33270 | biostudies-arrayexpress

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Project description:We used RNA-Seq to ask whether the transcripts for the proposed BCKDH subunits are upregulated in wild-type plants subjected to prolonged darkness. These experiments were performed using rosette leaves from 5-week-old, short-day-grown (8h light/16h dark) Col-0 wild-type plants moved to constant darkness for 6h, 24h, 48h and 72h, and grown in short day for 72h as control. The transcripts of eight BCAA catabolism genes were increased nine- to 400-fold within the first 6h of prolonged darkness, and remained high until the last time point. These results are consistent with the hypothesis that BCAA catabolic enzymes - including BCKDH subunits E1A1, E1B1, E1B2 and E2 - have one or more physiological roles in the dark.

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high temp wt - High temperature Arabidopsis root transcriptome

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Arabidopsis pvip mutants

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2009-12-11 | GSE19341 | GEO

Genome-wide analysis of the prmt5 and prmt4a;4b mutant transcriptomes

Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of prmt5 and prmt4a;4b Arabidopsis mutants with their respective wild type plants grown under continous light conditions WT, prmt5 and prmt4a;4b mutant plants were grown together with their respective wild type plants for two weeks under continuous white light at 22 degrees centigrades and the transcriptional profile of these plants was analyzed using RNA-seq.

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Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of gemin2 Arabidopsis mutant with wild type plants grown under continous light conditions

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