Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differentially expressed genes between oncogene-transformed and non-transformed fetal liver progenitor cells

ABSTRACT: The activation of different oncogenic signals may primarily contribute to the heterogeneity of cancer cells. However, the exact mechanisms underlying different oncogenic transformation are still unclear. We used the c-Myc, H-Ras and Akt transformed liver cell model to define mRNA expression profiles in the non-transformed and the three types of oncogene-transformed cells

ORGANISM(S): Mus musculus

PROVIDER: GSE43991 | GEO | 2013/02/02

SECONDARY ACCESSION(S): PRJNA188308

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Differentially expressed genes between oncogene-transformed and non-transformed fetal liver progenitor cells

Project description:The activation of different oncogenic signals may primarily contribute to the heterogeneity of cancer cells. However, the exact mechanisms underlying different oncogenic transformation are still unclear. We used the c-Myc, H-Ras and Akt transformed liver cell model to define mRNA expression profiles in the non-transformed and the three types of oncogene-transformed cells Purified p53-/- mouse fetal liver progenitor cells were transformed by three oncogenic genetic factors, c-Myc, H-RasV12 and myristoylated-AKT1(myr-Akt). The gene expression profilings of transformed cells and control cells were analyzed through microarray.

2013-02-02 | E-GEOD-43991 | biostudies-arrayexpress

Differentially expressed genes between oncogene-introduced wild-type and Atf4-/- cells

Project description:During oncogenic transformation, cells acquire genetic mutations that override the normal mechanisms regulating cellular proliferation. Excessive expression of activating transcription factor 4 (ATF4) is often observed in mammalian malignant tumors. However, little is known about the role of ATF4 in the malignant transformation of normal cells. Here, we show that ATF4 promotes oncogene-induced malignant transformation of murine fibroblasts by suppressing the expression of cellular senescence-associated genes. Ectopic expression of oncogenes, H-rasV12 and simian virus 40 large T-antigen, elicited malignant transformation of embryonic fibroblasts from wild-type mice, but not from Atf4-null (Atf4-/-) mice. The oncogenic stresses induced the expression of both Atf4 and cyclin-dependent kinase inhibitor 2a (Cdkn2a), in wild-type cells. Elevated levels of ATF4 successively suppressed the expression of cdkn2a encoding the cellular senescence-associated proteins, p16INK4a and p19ARF, thereby promoting oncogenic transformation. Conversely, the loss of ATF4 caused cellular senescence resulting from the consistent expression of p16INK4a and p19ARF. These findings reveal a novel function of ATF4: that of promoting oncogenic transformation by suppressing cellular senescence. Total 2 samples were derived from [1] H-Ras and SV40T expressing vector-transfected wild-type mouse embryonic fibroblasts (MEFs) and [2] H-Ras and SV40T expressing vector-transfected Atf4-/- MEFs

2011-08-16 | E-GEOD-31405 | biostudies-arrayexpress

Transcription profiling of mouse NIH3T3 cells were compared with myc/ras transformed NIH3T3 cells and with IRF1 expressing myc/ras transformed cells

Project description:Interferons have been ascribed to mediate antitumor effects. IRF-1 is a major target gene of interferons. It inhibits cell proliferation and oncogenic transformation. Here we show that 60% of all mRNAs deregulated by oncogenic transformation mediated by c-myc and H-ras are reverted to the expression levels of non-transformed cells by IRF-1. These include cell cycle regulating genes. Activation of IRF-1 decreases cyclin D1 expression and CDK4 kinase activity concomitant with dephosphorylation of pRb. These effects of IRF-1 are mediated by inhibition of the MEK-ERK pathway and a transcriptional repression of cyclin D1. IRF-1 mediated effects on cell cycle progression were found to be overridden by ectopic expression of cyclin D1. Ablation of cyclin D1 by RNA interference experiments prevents transformation and tumor growth in nude mice. The data demonstrate that cyclin D1 is a key target for IRF-1 mediated tumor suppressive effects. Experiment Overall Design: 3 samples were analysed, two replicates per sample. NIH3T3 cells were compared with myc/ras transformed NIH3T3 cells and with IRF1 expressing myc/ras transformed cells.

2008-06-14 | E-GEOD-6505 | biostudies-arrayexpress

A conserved microRNA signature related to cellular transformation by the RAS oncogene (miRNA)

Project description:The differential expression of microRNAs in Ras transformed epithelial cells of human and rat origin was investigated. Here we describe the group of miRNAs differentially expressed in two Ras transformed cell lines and defined it as a miRNA Ras signatures. We show that expression of these miRNAs are also affected in different primary tumors with high frequency of Ras mutation. We demonstate that introduction of different miRNAs lost during Ras transformation have big influence on cellular phenotype and mRNA expression. Importance of Ras pathway dependent transcription factors for miRNA regulation was shown as well. The complexity of miRNA – signal transduction pathways interactions and importance of miRNA regulation in Ras dependent malignant tumor formation is discussed.

2020-08-28 | GSE46794 | GEO

A conserved microRNA signature related to cellular transformation by the RAS oncogene (Affymetrix mRNA)

2020-08-28 | GSE46301 | GEO

Loss of immunosuppressive and anti-inflammatory properties in transformed human mesenchymal stem cells

Project description:We used collection of sequentially mutated BM-hMSCs ranging from wild type (no oncogenic hits) to fully transformed hMSCs (targeted with up six oncogenic mutations)to address whether BM-hMSCs at different stages of a well-characterized oncogenic process (normal, immortalized, transformed) retain immunomodulatory properties in vitro and in vivo. We characterize, for the first time, an oncogenic transformation-associated loss of the immunesuppressive and anti-inflammatory properties by hMSCs and identify candidate immune effectors underlying the loss of immunomodulation in transformed hMSCs.

2015-05-14 | GSE55108 | GEO

Identification of differentially expressed genes in MDV-transformed cells lines treated with sodium butyrate

Project description:In order to characterize alterations in cellular regulatory mechanisms associated with MDV reactivation, we employed microarray analysis to identify changes in gene expression in chemically-reactivated MDV transformed cell lines, specifically MSB1, RP2 and CU115. Differentially expressed genes identified in the MDV-transformed cell lines were then compared against differentially expressed genes in the REV-transformed CU91 cell line. This step was done to reduce the number of false positives (i.e. genes likely responding solely to sodium butyrate rather than MDV reactivation). Multiple cellular pathways are also affected by MDV reactivation, including those associated with apoptosis, the cell cycle, DNA repair, and immune response.

2018-08-23 | GSE103028 | GEO

Gene expression data from oncogene transformed murine prostate epithelial cell lines

Project description:Primary prostate epithelial cell cultures were established from the VENTRAL prostates of FVB mice . Cells were transduced with retroviral expression vectors encoding a single distinct oncogene (c-Myc, Ha-Ras (V-12), v-Src and NeuT, an activating mutant of ErbB2). Individual colonies of oncogene-transduced cells were selected and characterized. These cell lines demonstrate contact-independent growth characteristics in soft agar and in immune competent mice. To further characterize the molecular genetic signaling pathways regulated by specific oncogenes in prostate epithelial cells mRNA was prepared from the oncogene transformed PEC cell lines, 12 samples(each oncogene has 3 lines) and 3 normal prostate epithelial cell controls in good condition.

2013-04-01 | E-GEOD-37428 | biostudies-arrayexpress

Differentially expressed genes between oncogene-introduced wild-type and Atf4-/- cells

2011-08-17 | GSE31405 | GEO

Proteomic analysis of 3D cultures of fibroblasts and Ras-transformed HaCaT keratinocytes

Project description:HaCaT, and its three retrovirally c-Ha-ras-transformed subclones, A5, II-4 and RT3 (abbreviated as ras-HaCaTs in the text) that exhibit benign, invasive and malignant phenotypes, respectively, were cultured as 3D monocultures and co-cultures with fibroblasts. After hypotonic lysis of the cells, the insoluble proteins were analysed by LC-MS/MS.

2019-09-10 | PXD013113 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data