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Real-time quantitative PCR analysis of BMI1-silenced human prostate cancer cells

ABSTRACT: BMI1-silenced Prostate cancer cells exhibited a significant change in the expression of several proliferation-associated genes. These included Cyclin-D1, BCL2, IL and NF-kappaB. An increased expression of p16, p15 and TIMP3 in BMI1-silenced tumor cells was observed List of Selected Genes Modulated by BMI1-supression in Prostate cancer Cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE44049 | GEO | 2013/02/05

SECONDARY ACCESSION(S): PRJNA188447

REPOSITORIES: GEO

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Real-time quantitative PCR analysis of BMI1-silenced human prostate cancer cells

Project description:BMI1-silenced Prostate cancer cells exhibited a significant change in the expression of several proliferation-associated genes. These included Cyclin-D1, BCL2, IL and NF-kappaB. An increased expression of p16, p15 and TIMP3 in BMI1-silenced tumor cells was observed List of Selected Genes Modulated by BMI1-supression in Prostate cancer Cells qPCR gene expression profiling. LNCaP and BMI1-silenced LNCaP cells were used as indicated in the summary. Equal amount of total RNA from each group was pooled prior to gene expression analysis.

2013-02-05 | E-GEOD-44049 | biostudies-arrayexpress

RNA-seq and ChIP-seq analysis of BMI1 or RING1B-silenced prostate cancer cells C4-2

Project description:Purpose: Study of the role of BMI1 dependent and indpendent of PRC1 in castration-resistant prostate cancer(CRPC) Method: The expression of BMI1 or RING1B was silenced by 2 independent siRNA strands targeted at BMI1 or RING1B in C4-2 cells, scramble RNA as control. mRNA profiles and genome-wide chromatin-state maps were generated by deep sequencing. AR, BMI1 and IgG ChIP was conducted in C4-2 cells. Results: AR-induced genes were significantly down regulated by BMI1 knockdown but not RING1B. higher expression levels of BMI1 activated genes (those downregulated by BMI1 knockdown) were significantly associated with poorer disease-free and poorer overall survival.

2018-02-07 | GSE97831 | GEO

Transcriptome profiling of Bmi1 silenced-K562 CML cell line

Project description:The Bmi1 Polycomb protein is involved in the epigenetic repressive control of self renewal and survival of cancer initiating cells. In Chronic Myeloid Leukemia (CML), bmi1 expression increases gradually as the disease progresses from a chronic latent phase to a deadly blast crisis. We developped an inducible shRNA system to silence Bmi1 in the human K562 chronic myeloid leukemia (CML) cell line in order to identify new Bmi1-target genes.

2015-05-12 | GSE54262 | GEO

Transcriptome profiling of Bmi1 silenced-K562 CML cell line

2015-05-12 | E-GEOD-54262 | biostudies-arrayexpress

Elucidation of BMI1, RYBP and H2AK119ub interactomes in Glioblastoma

Project description:Identification of BMI1, RYBP and H2AK119UB interactome in Glioblastoma (GBM) to elucidate BMI1 roles independent of the PRC1-complex in GBM.

2021-09-09 | PXD022057 | Pride

Murine Proerythroblasts (ProEs): WT vs. Bmi1-/-

Project description:Transcriptional profiling of ProEs purified from wild type and Bmi1-/- mice. Two-condition experiment, WT vs. Bmi1-/- ProEs. Biological replicates: 3 WT replicates, 3 Bmi1-/- replicates.

2014-11-18 | E-GEOD-63413 | biostudies-arrayexpress

Gene expression in the liver of Bmi1 knockout mice

Project description:To explore the potential targets of Bmi1 in the liver development of hepatic carcinogenesis, we assayed the gene expression level in the liver of Bmi1 knockout mice. We isolated the liver tissue of Bmi1 WT and KO mice around 6-8 weeks. Then we extracted total RNA and run the microarray detection. Gene expression in Bmi1 KO mouse livers was compared with that in Bmi1 WT mouse livers to screen potential targets of Bmi1.

2018-03-29 | GSE97170 | GEO

Bmi1 mutant vs. WT dental epithelia

Project description:Transcriptional profiling of Bmi1 mutant dental epithelia including the stem cell compartment to determine which genes are upregulated in response to loss of Bmi1.

2013-04-12 | GSE45999 | GEO

Bmi1 mutant vs. WT dental epithelia

Project description:Transcriptional profiling of Bmi1 mutant dental epithelia including the stem cell compartment to determine which genes are upregulated in response to loss of Bmi1. Two condition experiment: dental epithelia homozygous null for Bmi1 and WT dental epithelia. 4 replicates each

2013-04-12 | E-GEOD-45999 | biostudies-arrayexpress

Gene expression in the Bmi1 knockout Huh7 and Hep3B cells

Project description:Bmi1 plays a pivotal role in hepatic carcinoma (HCC), but its targets in HCC is unknown. To screen the potential targets, we transfected HCC cell line Huh7 and Hep3B with Bmi1 shRNA lenti-virus. After confirming the Bmi1 was knocked down using western blotting, we extracted total RNA and then run the microarray detection. Gene expression profiles in Bmi1 KO cells were compared with those in Bmi1 WT cells to screen potential targets of Bmi1.

2018-03-29 | GSE97169 | GEO

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