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Genome-wide analysis of MAOA on Norepinphrine induced HCC gene expression

ABSTRACT: Gene analysis of HCC cells transfected with Lenti-vector, Lenti-MAOA and stimulated with Norepinephrine

ORGANISM(S): Homo sapiens

PROVIDER: GSE44094 | GEO | 2013/02/10

SECONDARY ACCESSION(S): PRJNA188579

REPOSITORIES: GEO

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Genome-wide analysis of MAOA on Norepinphrine induced HCC gene expression

Project description:Gene analysis of HCC cells transfected with Lenti-vector, Lenti-MAOA and stimulated with Norepinephrine Total mRNA obtained from SMMC-7721 cells transfected with Lenti-vector, Lenti-MAOA and stimulated with Norepinephrine

2013-02-10 | E-GEOD-44094 | biostudies-arrayexpress

The effect of IL-37 in HCC cell line Sk-hep1

Project description:We performed whole microarray expressiong prolife of Sk-hep1 cells which could over-express IL-37. This project is to explore the effect of interleukin-37 on hepatocellular carcinoma(HCC) development. We transfected human HCC cell line Sk-hep1 with lentiviral vector encoding IL-37 or control. The successfully transfected cells were sorted by FACS and the cells were further cultured for 24 hours. The cells were harvested and send for microarray analysis.

2020-02-06 | GSE144728 | GEO

The role of the ETS factor ERG in prostate cancer

Project description:Prostate cancer cell lines that express ERG acquire a neuron-like phenotype. The human prostate tumor cell line LNCap was transfected with lenti-ERG or control lenti-vector. Gene expression profiling was performed to establish the ERG-associated phenotype.

2014-03-18 | E-GEOD-55944 | biostudies-arrayexpress

Gene expression in the Bmi1 knockout Huh7 and Hep3B cells

Project description:Bmi1 plays a pivotal role in hepatic carcinoma (HCC), but its targets in HCC is unknown. To screen the potential targets, we transfected HCC cell line Huh7 and Hep3B with Bmi1 shRNA lenti-virus. After confirming the Bmi1 was knocked down using western blotting, we extracted total RNA and then run the microarray detection. Gene expression profiles in Bmi1 KO cells were compared with those in Bmi1 WT cells to screen potential targets of Bmi1.

2018-03-29 | GSE97169 | GEO

Expression data from human ovarian cancer cell line

Project description:LASS2 was used to identified as a novel suppressor in cancer development and progression. We explored roles of LASS2 in ovarian cancer invasion and metastasis. Lenti-LASS2 and Lenti-vector cells were constructed in 3AO cells through a lentiviral vector system. We preformed global gene expression analysis between Lenti-LASS2 and Lenti-vector cells in accordance with standard Aglient protocols to explore differentially expressed genes and signaling pathways involved by LASS2 overexpression.

2018-07-04 | GSE116562 | GEO

Gene expression profiles of WDR4 knockdown in HCC cells

Project description:HCC cell line, Huh-7 cells and HCC-LM3 cells, was transfected with sh-WDR4-2 or sh-NC for 48hr to knockdown WDR4 expression, and check the downstream mRNA changes.

2021-07-07 | GSE169394 | GEO

Gene expression profiles of METTL5-Wild type(WT) knockout(KO) in HCC cells

Project description:HCC cell line, Huh-7 cells and HCC-LM3 cells, was transfected with METTL5 sgRNA to knockout METTL5 expression., and check the downstream mRNA changes.

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The transcriptome-wide CPSF6-induced APA alterations in HCC cells

Project description:In this study, we showed that CPSF6 promoted HCC cell growth, migration and metastasis in vitro and in vivo. In order to understand the underlying mechanisms of CPSF6, we aimed to perform a APA profiling analysis in hepatocyte and hepatoma carcinoma cells under different CPSF6 expression conditions using 3T-seq method.

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Clorgyline treatment of VCaP cells

Project description:Purpose: Monoamine oxidase A (MAOA) is a mitochondrial enzyme that degrades neurotransmitters including serotonin and norepinephrine. Its inhibitors are commonly used to treat neurologic conditions including depression. Recently, we and others identified high expression of MAOA in normal basal prostate epithelium and in high grade primary prostate cancer (PCa). Inhibition of MAOA with an irreversible inhibitor, clorgyline, induced differentiation in primary cultures of epithelial cells from normal tissues and high grade cancers. Furthermore, clorgyline treatment inhibited several oncogenic pathways in PCa cells, suggesting a clinical value of MAOA inhibitors as a pro-differentiation and anti-oncogenic therapy for high risk PCa. Here, we extended our studies to a model of advanced PCa, VCaP cells, which were derived from castration-resistant metastatic PCa and express a high level of MAOA. Methods: The growth of VCaP cells in the presence or absence of clorgyline was evaluated in vitro and in vivo. Gene expression changes in response to clorgyline were determined by microarray and validated by quantitative real-time PCR. Results: Treatment with clorgyline in vitro inhibited growth and altered the transcriptional pattern of VCaP cells in a manner consistent with the pro-differentiation and anti-oncogenic effects seen in treated primary PCa cells. Src, beta-catenin, and MAPK oncogenic pathways, implicated in androgen-independent growth and metastasis, were significantly downregulated. Clorgyline treatment of mice bearing VCaP xenografts slowed tumor growth and induced transcriptome changes similar to those noted in vitro. Conclusions: Our results support the possibility that anti-depressant drugs that target MAOA might find a new application in treating PCa. Compound Based Treatment: Clorgyline treament of VCaP cells compound_treatment_design

2010-10-25 | E-GEOD-19822 | biostudies-arrayexpress

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2022-11-11 | PXD037159 | Pride

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