Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression analysis of sporulating Bacillus subtilis strain 168 deleted for prpE gene

ABSTRACT: Investigation of whole genome gene expression level changes in sporulating Bacillus subtilis 168 delta-prpE mutant, compared to the wild-type strain. The mutation engineered into this strain results in impaired germination of spores.

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

PROVIDER: GSE44125 | GEO | 2013/07/08

SECONDARY ACCESSION(S): PRJNA188792

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Expression analysis of sporulating Bacillus subtilis strain 168 deleted for prpE gene

Project description:Investigation of whole genome gene expression level changes in sporulating Bacillus subtilis 168 delta-prpE mutant, compared to the wild-type strain. The mutation engineered into this strain results in impaired germination of spores. A six chip study using total RNA extracted from three separate wild-type cultures of sporulating Bacillus subtilis 168 and three separate cultures of sporulating mutant strain, Bacillus subtilis 168 delta-prpE, in which prpE (yjbP BSU11630) gene coding for a protein phosphatase is deleted entirely. Each chip consists of four fields able to measure the expression level of 4,104 genes from Bacillus subtilis subsp. subtilis strain 168 NC_000964 with eight 60-mer probe pairs (PM/MM) per gene, with two-fold technical redundancy.

2013-07-08 | E-GEOD-44125 | biostudies-arrayexpress

Bacillus subtilis 168 moenomycin stimulon

Project description:Transcriptional response of Bacillus subtilis to moenomycin in wild-type 168.

2011-06-17 | GSE30001 | GEO

Bacillus subtilis 168 moenomycin stimulon

Project description:Transcriptional response of Bacillus subtilis to moenomycin in wild-type 168. Bacillus subtilis 168, WT (-MOE) vs. WT (+MOE). The experiment was conducted in triplicate using three independent total RNA preparations. Untreated samples were labeled with Alexa Fluor 555 and moenomycin treated samples were labeled with Alexa Fluor 647.

2011-06-17 | E-GEOD-30001 | biostudies-arrayexpress

Expression data from Bacillus subtilis 168 during growth

Project description:The gene expression of Bacillus subtilis 168 showed 3 major patterns including early expression, transition expression and late expression We monitored Bacillus subtilis gene expression by using microarray at differernt time points

2013-04-10 | GSE45933 | GEO

The condition-dependent transcriptome of Bacillus subtilis 168

Project description:Recent studies revealed the unsuspected complexity of the bacterial transcriptome but its systematic analysis across many diverse conditions remains a challenge. Here we report the condition-dependent transcriptome of the prototype strain B. subtilis 168 across 104 conditions reflecting the bacterium's life-styles. This data set composed of 269 tiling array hybridizations allowed to observe ~85% of the annotated CDSs expressed in the higher 30% in at least one hybridization and thus provide an excellent coverage of the transcriptome of this bacterium. In addition to the Genbank annotation 1583 new segments of the chromosome were identified as transcribed and have transcription levels reported in the data matrix. RNA samples prepared from B. subtilis 168 grown under 104 experimental conditions were reverse transcribed, labeled and hybridized to tiling arrays. Most hybridizations were performed in duplicate or triplicate using RNA isolated from independent cultures. All experiments were performed with strain BSB1 which is a tryptophan-prototrophic (trp+) derivative of the 168 trpC2 strain.

2012-03-07 | E-GEOD-27219 | biostudies-arrayexpress

Bacillus subtilis WalR (YycF) ChIP-chip Bacillus subtilis; Bacillus subtilis subsp. subtilis str. 168

Project description:Identification of the specific WalR (YycF) binding regions on the B. subtilis chromosome during exponential and phosphate starvation growth phases. The data serves to extend the WalRK regulon in Bacillus subtilis and its role in cell wall metabolism, as well as implying a role in several other cellular processes. For each sample analyzed in this study three biological replicates were performed. Three different samples were taken from a strain expressing the WalR-SPA protein as well as from wild-type (168) without a tagged WalR. Samples were taken from exponentially growing cells in low phosphate medium (LPDM) as well as from phosphate-limited cells (T2). Each sample compares ChIP DNA vs. Total DNA from the same cells.

2015-01-26 | E-GEOD-65272 | biostudies-arrayexpress

Expression data from Bacillus subtilis 168 during growth

2013-04-10 | E-GEOD-45933 | biostudies-arrayexpress