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Developing endosperm: Nipponbare vs NIL(TGW6)

ABSTRACT: Gene expression profoling of developping endosperm at 3 and 6 days after flowering (DAH): Nipponbare vs NIL(TGW6)

ORGANISM(S): Oryza sativa

PROVIDER: GSE44223 | GEO | 2013/02/12

SECONDARY ACCESSION(S): PRJNA189201

REPOSITORIES: GEO

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Developing endosperm: Nipponbare vs NIL(TGW6)

Project description:Gene expression profoling of developping endosperm at 3 and 6 days after flowering (DAH): Nipponbare vs NIL(TGW6) We used two rice varieties, NIL(TGW6) and Nipponbare.NIL(TGW6) is a nearly isogenic line containing the thousand grain weight qTGW6. For samples used three stages: 3 and 6 DAH endosperm in both Nipponbare and NIL(TGW6). Two dye swapped experiments were performed.

2013-02-12 | E-GEOD-44223 | biostudies-arrayexpress

Abscission layer (AL) at the base of flower in rice using laser micro-dissection: NIL(qSH1) AL vs. Nipponbare AL, NIL(qSH1) AL vs. NIL(qSH1) upper region of AL, and NIL(qSH1) AL vs. NIL(qSH1) lower region of AL

Project description:Tissue-specific transcriptional profiling of the abscission layer (AL) at the base of young flower in rice using laser micro-dissection: NIL(qSH1) vs. Nipponbare. We used two rice varieties, NIL(qSH1) and Nipponbare. NIL(qSH1) is a nearly isogenic line containing the seed shattering gene qSH1. Seed shattering is easy in NIL(qSH1), but it is not in Nipponbare. So, we used some stages of young flower in NIL(qSH1) and some in Nipponbare. Four regions: 1. abscission layer region of NIL(qSH1), 2. upper abscission region of NIL(qSH1), 3. lower abscission layer region of NIL(qSH1), and 4. abscission layer region of Nipponbare. Sample experiments: NIL(qSH1) AL vs. Nipponbare AL, NIL(qSH1) AL vs. NIL(qSH1) upper region of AL, and NIL(qSH1) AL vs. NIL(qSH1) lower region of AL.

2012-03-31 | E-GEOD-33409 | biostudies-arrayexpress

Project description:Tissue-specific transcriptional profiling of the abscission layer (AL) at the base of young flower in rice using laser micro-dissection: NIL(qSH1) vs. Nipponbare.

2012-04-01 | GSE33409 | GEO

Developing rice endosperm at seven days after flowering: Aleurone layers vs central starchy endosperm

Project description:Transcriptional profiling of developing rice endosperm at seven days after flowering comparing aleurone layers with central starchy endosperm. Cereal productivity is dependent on the accumulation of storage compounds in the endosperm, a nutritive tissue that is composed of aleurone cells in the outermost regions and starchy endosperm in the inner region. The transcriptional analyses provides clues to the molecular basis for different metabolic pathways in response to the spatial and nutritional differences between rice aleurone cells and starchy endosperm.

2012-11-27 | GSE42489 | GEO

Comparative gene expression analysis between Nipponbare and 6-4 under two conditions

2009-10-31 | GSE6187 | GEO

Developing rice endosperm at seven days after flowering: Aleurone layers vs central starchy endosperm

2012-11-27 | E-GEOD-42489 | biostudies-arrayexpress

Oryza sativa

Project description:Developing endosperm: Nipponbare vs NIL(TGW6)

| PRJNA189201 | ENA

Transcription profiling of rice varieties Nipponbare and 6-4 grown in either phosphorus deficient or fertilized soil

Project description:Nipponbare performs poorly in phosphorus (P) deficient soil whereas a Nipponbare-derived NIL containing the Pup1 allele of donor parent Kasalath is tolerant to P deficiency. In this experiment we compared gene expression patterns in roots of this NIL to Nipponbare, grown either in a P deficient or P fertilized soil. The aim is to separate constitutive differences in expression from those induced by P deficiency. Experiment Overall Design: Total RNA was extrancted from root that were grown under two conditions and prepared three biological replicant in each line (total 6 samples in each line). Experiment Overall Design: We used 6 slides for this experiment, in each slide, Nipponbare and 6-4 samples grown under same condition were hybridized.

2009-11-10 | E-GEOD-6187 | biostudies-arrayexpress

RNA-Seq Analysis of Laser-Capture Microdissected Cells of the Developing Central Starchy Endosperm of Maize

Project description:Endosperm is a product of double fertilization, and provides nutrients and signals to the embryo during seed development in flowering plants. Early stages of endosperm development are critical for the development of its storage capacity through synthesis and accumulation of starch and storage proteins. Here we report on the isolation and sequencing of mRNAs from the central portion of the starchy endosperm of Zea mays (maize) B73 at 6 days after pollination. We detected high correlation among the four biological replicates of RNAs isolated using laser-capture microdissection of the cell type. Because the assayed stage of development precedes the synthesis and accumulation of the major storage proteins and starch in the endosperm, our dataset likely include mRNAs for genes that are involved in control and establishment of these developmental programs.

2014-06-17 | GSE58504 | GEO

RNA-Seq Analysis of Laser-Capture Microdissected Cells of the Developing Central Starchy Endosperm of Maize

2014-06-17 | E-GEOD-58504 | biostudies-arrayexpress

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