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Gene expression in human fungal pathogen Coccidioides immitis changes as mycelia differentiate into spherules and mature

ABSTRACT: Coccidioides immitis (C. immitis) is a dimorphic fungus that causes disease in mammals including human beings. It grows as a mycelium in the soil but differentiates into a pathogenic structure called a spherule in the host. We compared the transcriptome of C. immitis mycelia and day 2 and day 8 spherules grown in vitro using a custom custom oligonucleotide microarray from Nimblegen.

ORGANISM(S): Coccidioides immitis RS

PROVIDER: GSE44225 | GEO | 2013/07/11

SECONDARY ACCESSION(S): PRJNA189205

REPOSITORIES: GEO

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Gene expression in human fungal pathogen Coccidioides immitis changes as mycelia differentiate into spherules and mature

Project description:Coccidioides immitis (C. immitis) is a dimorphic fungus that causes disease in mammals including human beings. It grows as a mycelium in the soil but differentiates into a pathogenic structure called a spherule in the host. We compared the transcriptome of C. immitis mycelia and day 2 and day 8 spherules grown in vitro using a custom custom oligonucleotide microarray from Nimblegen. C. immitis RS strain isolated from infected mice was grown on agar. Arthroconidia was harvested and inoculated into mycelial spores. RNA was extracted from spores at different days post-inoculation and hybridized to a custom Nimblegen array, in order to identify differentially expressed genes between mycelia and spherules.

2013-07-11 | E-GEOD-44225 | biostudies-arrayexpress

Transcriptional analysis of Coccidioides immitis mycelia and spherules by RNA sequencing

Project description:Coccidioides immitis and posadasii are dimorphic fungi that transform from mycelia with internal arthroconidia in the soil to a tissue form known as a spherule. Genes that were highly up-regulated in young spherules include a spherule surface protein and iron and copper membrane transporters. Genes that are unique to Coccidioides spp. are also over-represented in this group, suggesting that they may be important for spherule differentiation. Enriched GO terms in up-regulated genes in young spherules include oxidation/reduction, response to stress and membrane proteins. Down-regulated genes are enriched for transcription factors, especially helix-loop-helix and C2H2 type zinc finger domain-containing proteins which is consistent with the dramatic change in transcriptional profile. Almost all genes that are up-regulated in young spherules remain up-regulated in mature spherules, but a small number of genes are differentially expressed in those two stages of spherule development. Mature spherules express more Hsp31, and amylase than young spherules and less tyrosinase. Some expression of transposons was detected and most of the differentially expressed transposons were up-regulated in spherules.

2021-04-01 | GSE171286 | GEO

Transcriptional analysis of Coccidioides immitis mycelia and spherules by RNA sequencing

Project description:Transcriptional analysis of Coccidioides immitis mycelia and spherules by RNA sequencing

| PRJNA718949 | ENA

Coccidioides immitis RS

Project description:Gene expression in human fungal pathogen Coccidioides immitis changes as mycelia differentiate into spherules and mature

| PRJNA189205 | ENA

Coccidioides immitis

Project description:Coccidioides immitis Raw sequence reads

| PRJNA820037 | ENA

Proteogenomic Re-annotation of Coccidioides posadasii strain Silveira

Project description:The aims of this study were to present modifications to the annotations of the genome of C. posadasii, one of two closely related species of Coccidioides, a dimorphic fungal pathogen that causes coccidioidomycosis, also called Valley Fever. Proteins present in lysates and filtrates of in vitro grown mycelia and parasitic phase spherules from C. posadasii strain Silveira were analyzed using a GeLC-MS/MS method.

2017-11-08 | PXD006489 | Pride

Factors regulated by interferon gamma and hypoxia-inducible factor 1A contribute to responses that protect mice from Coccidioides immitis infection.

2012-09-10 | GSE40379 | GEO

Factors regulated by interferon gamma and hypoxia-inducible factor 1A contribute to responses that protect mice from Coccidioides immitis infection.

Project description:Mouse strains have been identified that are resistant (i.e. DBA/2) or susceptible (i.e. C57BL/6) to infection from pathogenic fungus Coccidioides immitis. However, the genetic and immunological basis for this difference has not been fully characterized. Microarray technology was used to identify genes that were differentially expressed in lung tissue between resistant DBA/2 and sensitive C57BL/6 mice after infection with C. immitis. C57BL/6 and DBA/2 mice were infected with arthoconidia of C. immitis and RNA was extracted at different days post-infection and hybridized to Affymetrix microarrays (MGU74Av2), in order to identify differentially expressed genes between the two strains.

2012-09-10 | E-GEOD-40379 | biostudies-arrayexpress

RNA-seq transcriptional analysis of mouse lung tissue with and without Coccidioides immitis lung infection

Project description:Our previous data suggested that CXC ELR+ chemokines might be involved in neutrophil recruitment to the lungs during Coccidioides infection. To test that hypothesis, we infected IL-8R2 (Cxcr2) KO mice on a BALB/c background. IL-8R2 KO mice had fewer neutrophils in infected lungs than BALB/c controls, but unexpectedly the IL-8R2 KO mice also had 10-fold fewer organisms in their lungs than did control mice. To better understand the differences in IL-8R2 KO mouse lungs during Coccidioides infection we performed RNA-seq on lung tissue from uninfected and Coccidioides immitis infected mouse lungs in control and IL-8R2 (Cxcr2) KO mice. IL-8R2 KO mouse lungs had higher expression of genes associated with lymphocyte activation, including Th1 and Th17-related genes Ifnγ and Il17a and transcription factors Stat1 and Rorc. Bronchial alveolar lavage (BAL) fluid from infected IL-8R2 KO mice similarly contained more IL-17A and IFNγ.

2020-09-14 | GSE146594 | GEO

AÂ Coccidioides posadasii CPS1Â deletion mutant is avirulent and protects mice from lethal infection

Project description:TheÂ CPS1Â gene was identified as a virulence factor in the maize pathogen, Cochliobolus heterostrophus. Hypothesizing that the homologous gene in Coccidioides posadasiiÂ (Cp) could be important for virulence, we created a deletion mutant,Â Îcps1, which was unable to cause disease in three strains of mice (C57BL/6, BALB/c, or the severely immunodeficient NOD-scid,Î³cnullÂ [NSG]). Only a single colony was recovered from one of 60 C57BL/6 mice following intranasal infections of up to 4400 spores. Following administration of very high doses (10,000 to 2.5 x 10^7Â spores) to NSG and BALB/c mice, spherules were observed in lung sections at time points from day 3 to day 10 post-infection, but nearly all appeared degraded with infrequent endosporulation. Although the role ofÂ CPS1Â in virulence is not understood, phenotypic alterations and transcription differences of at least 33 genes inÂ Îcps1Â vs. Cp is consistent with both metabolic and regulatory functions for the gene. The inÂ vitroÂ phenotype ofÂ Îcps1Â showed slower growth of mycelia with delayed and lower spore production compared to Cp, andÂ in vitro spherules were smaller. Vaccination of C57BL/6 or BALB/c mice with liveÂ Îcps1 spores either intranasally, intraperitoneally or subcutaneously resulted in over 95% survival with mean residual lung fungal burdens <1000 colony-forming units from an otherwise lethal Cp intranasal infection. Considering its apparently complete attenuation of virulence and the high degree of resistance to Cp infection when used as a vaccine,Â Îcps1Â is a promising vaccine candidate for preventing coccidioidomycosis in humans or other animals. Wild type and CPS1 deletion mutant strains of Coccidioides posadasii strain Silveira spherules grown for 48 hours in Converse medium at 38 degrees celsius in duplicate. RNAseq was performed on an Illumina HiSeq2000 (2x100 paired end).

2016-08-15 | E-GEOD-85364 | biostudies-arrayexpress

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