Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Beta cell 5’-shifted isomiRs are candidate regulatory hubs in type 2 diabetes

ABSTRACT: We performed deep sequencing of small RNA from mouse insulinoma (MIN6) cells cultured in 25mM glucose. We then developed and implemented an in-house short-read mapping strategy to analyze isomiR diversity.

ORGANISM(S): Mus musculus

PROVIDER: GSE44262 | GEO | 2013/09/10

SECONDARY ACCESSION(S): PRJNA189264

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Beta cell 5’-shifted isomiRs are candidate regulatory hubs in type 2 diabetes

Project description:We performed deep sequencing of small RNA from mouse insulinoma (MIN6) cells cultured in 25mM glucose. We then developed and implemented an in-house short-read mapping strategy to analyze isomiR diversity. Profile of miRNA expression in MIN6 cells cultured in 25mM glucose.

2013-09-10 | E-GEOD-44262 | biostudies-arrayexpress

Beta cell 5'-shifted isomiRs are candidate regulatory hubs in type 2 diabetes.

Project description: Not available

| S-EPMC3767796 | biostudies-literature

Addressing bias in small RNA library preparation for sequencing: a new protocol recovers microRNAs that evade capture by current methods

Project description:We performed deep sequencing of small RNA from mouse insulinoma (MIN6) cells or mouse liver using different library prepartion methods.

2015-12-04 | GSE75457 | GEO

Addressing bias in small RNA library preparation for sequencing: a new protocol recovers microRNAs that evade capture by current methods

Project description:We performed deep sequencing of small RNA from mouse insulinoma (MIN6) cells or mouse liver using different library prepartion methods. Libraries were preapred using MIN6 or Mouse Liver total RNA using (1) Illumina v1.5 small RNA library prep kit, (2) Illumina TruSeq kit, or (3) Bioo Scientific NEXTFlexV2

2015-12-04 | E-GEOD-75457 | biostudies-arrayexpress

Adaptation to chronic ER stress enforces pancreatic beta-cell plasticity

Project description:The next-generation-sequencing (NGS) of ribosome footprintings and poly(A) enriched RNA-seq have been proformed to examine transcriptional and translational changes in an mouse insulinoma cell MIN6 under acute and chronic endoplasmic reticular stresses.

2021-05-20 | GSE174679 | GEO

Human MDA-MB-231 breast cancer cells overexpressing miR-183-5p and its isomiRs

Project description:Overexpression of miR-183-5p|+2, but not of the other two isomiRs |0 and |+1, was observed to reduce cell cycle and cell proliferation in different triple-negative breast cancer cell lines. Therefore, we hypothesized that the |+2 isoform has targets distinct from the other two isoforms. To test this hypothesis, we overexpressed separately the three different isoforms or negative controls (siAllstar or mimic-Cltr) and performed Mass Spectrometry to identify differentially regulated proteins. Interestingly, a gene set enrichment analysis of the changes in protein expression revealed significant downregulation of transcriptional targets of E2F specifically in cells transfected with the |+2 isoform prompting us to validate the predicted isomiR specific target E2F1. Subsequently, we could show that direct targeting of E2F1 by miR-183-5p|+2 is responsible for the impact of the isomiR on cell cycle and proliferation.

2022-05-26 | PXD026763 | Pride

RNA-Seq of mouse beta cell line MIN6 after siRNA knockdown of lncRNA Pax6os1

Project description:Pax6os1 was knocked using by a pool of 3 siRNAs in the mouse insulinoma (beta cell) line MIN6. A parallel experiment using 3 control siRNAs was performed and RNA extracted 72 hours after transfection and subjected to RNA-Seq.

2021-06-01 | E-MTAB-9218 | biostudies-arrayexpress

Modulation of insulin secretion through RBFOX2-mediated alternative splicing [eCLIP-Seq MIN6]

Project description:Recent studies have identified dysregulation of RNA-binding proteins (RBPs) and aberrant mRNA splicing in the onset of diseases including diabetes. Here we investigated the role of RBFOX2 in the pancreatic β cell through the conditional mutation of Rbfox2 in the mouse pancreas (Pdx1:Cre; Rbfox2fl/lf) and RNAi experiments in the mouse insulinoma cell line, MIN6. We then identified the direct targets of RBFOX2 in the mouse β cell transcriptome my eCLIP-Seq in MIN6 cells.

2023-09-25 | GSE221276 | GEO

Modulation of insulin secretion through RBFOX2-mediated alternative splicing [RNA-seq Mouse Iselt]

2023-09-25 | GSE221275 | GEO

Modulation of insulin secretion through RBFOX2-mediated alternative splicing [RNA-seq MIN6]

2023-09-25 | GSE221274 | GEO