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Effects of PSF and CTBP1-AS knockdown in prostate cancer cell lines

ABSTRACT: Prostate cancer is the most common cancer in men and Androgen receptor (AR) downstream signalings promote prostate cancer cell proliferation. We identified androgen-regulated long non-coding RNA, CTBP1-AS, located in the antisese region of CTBP1 gene. CTBP1-AS activate AR signaling by epigenetically repress AR-associated cofactors such as CTBP1 by interactign with RNA-binding protein PSF and recruiting HDAC complex to the target promoters. In order to investigate the CTBP1-AS and PSF function in prostate cancer cells, we performed gene expression in AR-positive prostate cancer cell lines after siPSF or siCTBP1-AS treatment. We also treated cells with vehicle or androgen to analyzed the effects of CTBP1-AS and PSF on AR function.

ORGANISM(S): Homo sapiens

PROVIDER: GSE44264 | GEO | 2014/01/01

SECONDARY ACCESSION(S): PRJNA189266

REPOSITORIES: GEO

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Effects of PSF and CTBP1-AS knockdown in prostate cancer cell lines

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