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Differential miRNA profiles of pregnancy tissue in ectopic pregnancies

ABSTRACT: Abstract Objective: To investigate the physiology of ectopic pregnancy by analyzing the miRNA profiles of the pregnancy tissue from both ectopic and control pregnancies (voluntary termination of pregnancy). Design: Research study. Setting: Academic research institute in collaboration with a university hospital. Patients: Patients suffering from tubal EP and patients with a normal ongoing pregnancy scheduled for a voluntary termination of pregnancy (VTOP) were recruited. Interventions: Pregnancy tissue samples were analyzed by miRNA microarray and further validated by qPCR. Main Outcome measures: Gene expression profiles and quantitative PCR measurements. Results: Four miRNAs were found to be downregulated in EP compared to healthy trophoblast tissue, and three miRNAs were upregulated in EP trophoblast compared to control tissue samples. All genes were validated by q-PCR. We found three statistically significant miRNAs, all of which were upregulated in the EP sample group. Conclusion: We describe the alteration of seven miRNAs in EP samples that could alter pathways which are critical for correct implantation such as extracellular matrix (ECM) remodeling, or mucin biosynthesis, thus resulting in ectopic pregnancies.

ORGANISM(S): Homo sapiens

PROVIDER: GSE44731 | GEO | 2014/06/02

SECONDARY ACCESSION(S): PRJNA191104

REPOSITORIES: GEO

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Differential miRNA profiles of pregnancy tissue in ectopic pregnancies

Project description:Abstract Objective: To investigate the physiology of ectopic pregnancy by analyzing the miRNA profiles of the pregnancy tissue from both ectopic and control pregnancies (voluntary termination of pregnancy). Design: Research study. Setting: Academic research institute in collaboration with a university hospital. Patients: Patients suffering from tubal EP and patients with a normal ongoing pregnancy scheduled for a voluntary termination of pregnancy (VTOP) were recruited. Interventions: Pregnancy tissue samples were analyzed by miRNA microarray and further validated by qPCR. Main Outcome measures: Gene expression profiles and quantitative PCR measurements. Results: Four miRNAs were found to be downregulated in EP compared to healthy trophoblast tissue, and three miRNAs were upregulated in EP trophoblast compared to control tissue samples. All genes were validated by q-PCR. We found three statistically significant miRNAs, all of which were upregulated in the EP sample group. Conclusion: We describe the alteration of seven miRNAs in EP samples that could alter pathways which are critical for correct implantation such as extracellular matrix (ECM) remodeling, or mucin biosynthesis, thus resulting in ectopic pregnancies. This study was authorized by the Institutional Review Board/Independent Ethics Committee of the Hospital Universitario La Fe, Valencia, Spain. Eight patients suffering from tubal EP and another eight patients with a normal ongoing pregnancy scheduled for a VTOP were recruited.

2014-06-02 | E-GEOD-44731 | biostudies-arrayexpress

Tubal ectopic pregnancy as a model to identify endometrial genes and signaling pathways important in decidualization and regulated by local trophoblast

Project description:Gestation-matched endometrium was collected from women with ectopic pregnancy (EP) (n=11) and intrauterine pregnancies (IUP) (n=13). RNA was extracted from the tissue. In addition, tissues were prepared for histological analysis for degree of decidualization. We compared a) the samples from EP that were decidualized (n=6) with non-decidualized samples (n=5), and b) the decidualized EP (n=6) with decidualization-matched IUP (n=6) samples.

2011-07-01 | E-MTAB-680 | biostudies-arrayexpress

Homo sapiens

Project description:Differential miRNA profiles of pregnancy tissue in ectopic pregnancies

| PRJNA191104 | ENA

Molecular expression in endometrial biopsies of non-viable ectopic and intrauterine pregnancies.

Project description:Ectopic pregnancy is the leading cause of morbidity and mortality among women in the first trimester. A reliable diagnostic test for the detection of ectopic pregnancy in cases of non-viable pregnancy of unknown location (NV-PUL) is needed. The objective of the current study is to prospectively validate our previous findings of a 12-cilia gene classifier for the identification of ectopic pregnancy. Molecular interrogation is performed to define differences in molecular expression profiles in ectopic and abnormal intrauterine pregnancies. These samples come from a prospective cohort study comparing transcriptome profiles from women with ectopic pregnancy (ECT) or non-viable intrauterine pregnancy (NV-IUP). Samples were analyzed with the Affymetrix Human Gene 2.0 ST Array.

2019-05-21 | GSE131529 | GEO

Proteome-defined changes in cellular pathways for decidua and trophoblast tissues associated with location and viability of early-stage pregnancy

Project description:A label-free proteome analysis comparing changes in protein profiles of decidualized endometria and trophoblasts among women with different pregnancy outcomes, specifically a viable intrauterine pregnancy (IUP), ectopic pregnancy (EP), or early pregnancy loss (EPL), that identify potential biomarkers and provide insights into cellular pathways affected by fetus viability and location.

2021-12-16 | MSV000088572 | MassIVE

Differences in molecular expression in cases of non-viable ectopic and intrauterine pregnancies

Project description:Ectopic pregnancy is the leading cause of morbidity and mortality among women in the first trimester. A reliable diagnostic test for the detection of ectopic pregnancy in cases of non-viable pregnancy of unknown location (NV-PUL) is needed. The objective of the current study is to define differences in molecular expression profiles in ectopic and abnormal intrauterine pregnancies toward discovery of biologically plausible ectopic classifier candidate. These samples come from a prospective cohort study comparing transcriptome profiles from women with ectopic pregnancy (ECT) or non-viable intrauterine pregnancy (NV-IUP). Samples were analyzed with the Affymetrix Human Gene 2.0 ST Array. The views expressed are those of the author(s) and do not reflect the official policy of the Department of the Army, the Department of Defense or the U.S. Government. The investigators have adhered to the policies for protection of human subjects as prescribed in 45 CFR 46.

2018-05-31 | GSE112879 | GEO

Hypoxia induces trophoblast migration and invasion gene pathways, a subset of which are reduced in preeclampsia

Project description:Placentation requires the proper regulation of extravillous trophoblast (EVT) migration and invasion into the decidua and maternal vasculature, processes which are initiated in physiologic hypoxic conditions. Abnormal EVT migration and/or invasion have been suggested to lead to pregnancy complications, such as preeclampsia. The objectives of this study are to determine how exposure to hypoxia impacts gene expression and cellular motility of first trimester trophoblasts, and to assess if expression of migration-associated genes is dysregulated in 2nd trimester chorionic villous samples (CVS) from preeclampsia pregnancies relative to CVS from healthy pregnancies. The 1st trimester trophoblast cell line, HTR8/SVneo, was used to investigate the relationship between hypoxia and Notch signaling in trophoblast migration and invasion. RNA sequencing and quantitative RT-PCR analyses show that exposure to hypoxia (2.5% O2) activates Notch signaling in HTR-8/SVneo. We demonstrate that exposure of HTR-8/SVneo to hypoxia induces expression of genes associated with cellular migration and invasion and increases HTR-8/SVneo cellular migration and invasion, whereas inhibition of gamma-secretase decreases Notch signaling and decreases HTR-8/SVneo migration and invasion. Analysis of RNA sequencing data from CVS of preeclampsia and uncomplicated pregnancies identified significant differentially expressed genes that are involved in cellular migration and invasion. Decreased expression of migration and invasion genes in CVS from preeclampsia pregnancies, may impair trophoblast migration and invasion in the 2nd trimester of pregnancy, resulting in the development of preeclampsia.

2021-08-05 | GSE155750 | GEO

miRNA Expression in Early Porcine Pregnancy

Project description:This study examined the expression of pig-specific microRNAs (miRNAs) at gestation day 20 (gd20) of pregnancy in Yorkshire sows. Tissue differences in miRNA expression, and miRNA differences between healthy and arresting embryo attachment sites (i.e., healthy endometrium vs. arresting endometrium; healthy trophoblast vs. arresting trophoblast), were of prime interest. For more information, please refer to the primary research paper. Paired endometrium and trophoblast samples were collected at gestation day 20 from two conceptus attachement sites (1 healthy, 1 arresting) per sow (n=3). Endometrial samples were collected from four non-pregnant sows at mid-estrus.

2013-08-01 | E-GEOD-45761 | biostudies-arrayexpress

Single-cell atlas of patient-derived trophoblast organoids (TO) in on-going pregnancies.

Project description:Trophoblast organoids (TOs) hold great promise for elucidating human placental development and function. By deriving TOs in on-going pregnancies using chorionic villus sampling (CVS), we established a platform to study trophoblast differentiation and function in early pregnancy, including pregnancies with different fetal genetic abnormalities. We addressed cellular heterogeneity of CVS-derived TOs by providing a single-cell transcriptomic atlas and show that CVS-TOs recapitulate key aspects of the human placenta, including syncytial fusion and hormone synthesis. This study demonstrates the utility of trophoblast organoids for investigating genetic defects in the placenta and describes an experimental platform for future personalized placental medicine approaches, including genotype-phenotype mapping.

2022-10-01 | GSE211429 | GEO

Transcription profiling of human adipose tissue, decidua and placenta from patients with preeclampsia vs. unaffected indviduals reveals dysregulation of the circulating and tissue-based renin-angiotensin system in preeclampsia

Project description:Preeclampsia complicates more than 3% of all pregnancies in the United States and Europe. High-risk populations include women with diabetes, dyslipidemia, thrombotic disorders, hyperhomocysteinemia, hypertension, renal diseases, previous preeclampsia, twin pregnancies, and low socioeconomic status. In the latter case, the incidence may increase to 20% to 25%. Preeclampsia is a major cause of maternal and fetal morbidity and mortality. Preeclampsia is defined by systolic blood pressure of more than 140 mm Hg and diastolic blood pressure of more than 90 mm Hg after 20 weeks gestation in a previously normotensive patient, and new-onset proteinuria. Abnormal placentation associated with shallow trophoblast invasion (fetal cells from outer cell layer of the blastocyst) into endometrium (decidua) and improper spiral artery remodeling in the decidua are initial pathological steps. In this study we analyzed the renin-angiotensin system in adipose tissue, decidua and placenta from women with uneventful pregnancy and women with preeclampsia. We also analyzed the tissue by Affymetrix chips in a comparison study (control vs. preeclampsia) Experiment Overall Design: 6 affymetrix human expression chips (GPL570) were analyzed. Experiment Overall Design: They represent 3 tissues (pooled from 10 individuals each) from patients with preeclampsia and from patients with uneventful pregnancy (collected by cesaraen section). Tissues from patients with uneventful pregnancy are the controls in comparison to tissues of patients with preeclampsia.

2008-06-14 | E-GEOD-6573 | biostudies-arrayexpress

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