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Characterization of A. baumannii Mitomycin C (MMC) response

ABSTRACT: Transcriptional profiling of A. baumannii ATCC 17978 comparing treated-MMC cultures with non-MMC treated cultures

ORGANISM(S): Acinetobacter baumannii ATCC 17978

PROVIDER: GSE44735 | GEO | 2013/11/14

SECONDARY ACCESSION(S): PRJNA191140

REPOSITORIES: GEO

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Characterization of A. baumannii Mitomycin C (MMC) response

Project description:Transcriptional profiling of A. baumannii ATCC 17978 comparing treated-MMC cultures with non-MMC treated cultures Two-condition experiment A. baumannii 17978 MMC+ vs A. baumannii 17978 MMC-. Biological replicates:3, Technical replicates:2

2013-11-14 | E-GEOD-44735 | biostudies-arrayexpress

Transcriptional response to carbapenem treatment in Acinetobacter baumannii

Project description:We report transcriptome sequencing analysis on A. baumannii strain ATCC 17978 cultures treated with or without meropnem at 0.5, 3 and 9 h, in triplicate.

2022-05-20 | GSE190441 | GEO

Transcriptomic analysis of chlorhexidine shock in Acinetobacter baumannii

Project description:In this study the transcriptomic changes occurring in A. baumannii ATCC 17978 in response to shock treament using the biocide chlorhexdine were determined. A. baumannii ATCC 17978 cells were grown at 37°C with shaking (200rpm) in 35mL cultures in MH broth to OD600=0.75, at which time they were split into 15mL cultures. One 15mL sample was treated with 4µg/mL chlorhexidine (0.5x MIC), whereas the other was not treated and used as a reference. Cultures were allowed to grow for a further 30 mins (to an average final OD600= ~1.35 for untreated cells and 1.25 for chlorhexidine treated samples), when cells were harvested by centrifugation and immediately suspended in Trizol reagent (Invitrogen). Total RNA was extracted using the PureLinkTM Micro-to-Midi Total RNA Purification kit (Invitrogen), incorporating an on-column DNAseI (Invitrogen) digestion.

2013-12-03 | GSE51525 | GEO

Transcriptional responses of Acinetobacter baumannii ATCC 17978 to limiting iron availability

Project description:A. baumannii ATCC 17978 cells were incubated under iron replete (mueller-hinton) and iron limiting (MH + 200 ÂµM 2,2'-dipyridyl) conditions, total RNA was extracted when cultures reached OD600=0.7. The probes on the microarray cover all predicted open reading frames (at least 4 per ORF) and additional replicates of housekeeping genes of the A. baumannii ATCC 17978 genome

2013-10-18 | E-GEOD-24921 | biostudies-arrayexpress

Characterization of two Acinetobacter baumannii rifampin-spontaneous mutants lacking twitching motility

Project description:Transcriptional profiling of A. baumannii ATCC 17978 comparing two spontaneous rpoB mutants (Rif5 and Rif 8) with the wild-type parental strain

2017-06-12 | GSE73193 | GEO

Transcriptomic analysis of chlorhexidine shock in Acinetobacter baumannii

Project description:In this study the transcriptomic changes occurring in A. baumannii ATCC 17978 in response to shock treament using the biocide chlorhexdine were determined. A. baumannii ATCC 17978 cells were grown at 37Â°C with shaking (200rpm) in 35mL cultures in MH broth to OD600=0.75, at which time they were split into 15mL cultures. One 15mL sample was treated with 4Âµg/mL chlorhexidine (0.5x MIC), whereas the other was not treated and used as a reference. Cultures were allowed to grow for a further 30 mins (to an average final OD600= ~1.35 for untreated cells and 1.25 for chlorhexidine treated samples), when cells were harvested by centrifugation and immediately suspended in Trizol reagent (Invitrogen). Total RNA was extracted using the PureLinkTM Micro-to-Midi Total RNA Purification kit (Invitrogen), incorporating an on-column DNAseI (Invitrogen) digestion. The experiment included using four grids of a custom Agilent 8 x 15000 spot microarray. Three biological replicates and one flip dye experiment.

2013-12-03 | E-GEOD-51525 | biostudies-arrayexpress

Transcriptional responses of Acinetobacter baumannii ATCC 17978 to limiting iron availability

Project description:A. baumannii ATCC 17978 cells were incubated under iron replete (mueller-hinton) and iron limiting (MH + 200 µM 2,2'-dipyridyl) conditions, total RNA was extracted when cultures reached OD600=0.7.

2013-10-18 | GSE24921 | GEO

Expression Data from Acinetobacter baumannii strain ATCC 17978 and isogenic lpsB mutant cells

Project description:lpsB encodes a glycosyltransferase involved in lipopolysaccharide (LPS) synthesis. LPS is a major component of the Gram-negative bacterial outer membranes. We used custom-made Affymetrix A. baumannii strain ATCC 17978 derived GeneChips to compare the gene expression properties of wild type and isogenic lpsB mutant cells. Two mutants were evaluated; A. baumannii strain 5A7 is a ATCC 17978 derivative harboring a transposon (Tn5) within lpsB (A1S_0430 locus); A. baumannii strain containing a deletion of lpsB (A1S_0430). A. baumannii strain ATCC 17978, 5A7 (lpsB:Tn5) or IH1∆lpsB (∆lpsB) were grown to mid-exponential phase growth, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the transcription profile of lpsB mutated cells.

2012-12-01 | E-GEOD-40771 | biostudies-arrayexpress

Expression Data from Acinetobacter baumannii strain ATCC 17978 and isogenic lpsB mutant cells

2012-12-01 | GSE40771 | GEO

Transcriptome study of Acinobacter baumannii ddrR mutants in the presence or absence of mitomycin C

Project description:All RNA samples were isolated from the ddrR mutant strain of Acinobacter baumannii. The triplicates labeled “plus” have been subjected to mitomycin C (MMC) exposure (treated) and the triplicates labeled “minus” were not exposed to MMC (control). A comparison between treated and control samples to determine differential gene expression was performed.

2020-06-10 | GSE104741 | GEO

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