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DNA-damaging agent and immune inducer synergistically induced defence gene expression in Arabidopsis

ABSTRACT: We used microarrays to identify differentially expressed genes after DNA-damaging agent bleomycin (BLM) and/or immune inducer 2, 6-dichloroisonicotinic acid (INA) treatment. We focused on those genes that were synergistically induced by co-treatment (BLM+INA).

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE44793 | GEO | 2013/11/14

SECONDARY ACCESSION(S): PRJNA191812

REPOSITORIES: GEO

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DNA-damaging agent and immune inducer synergistically induced defence gene expression in Arabidopsis

Project description:We used microarrays to identify differentially expressed genes after DNA-damaging agent bleomycin (BLM) and/or immune inducer 2, 6-dichloroisonicotinic acid (INA) treatment. We focused on those genes that were synergistically induced by co-treatment (BLM+INA). Arabidopsis seedlings were treated with 4 μg/ml BLM and/or low INA (10 μM). There are 4 treatments: control (CK), INA, BLM and BLM+INA. Each treatments have three biological replicates. There are 12 samples in total.

2013-11-14 | E-GEOD-44793 | biostudies-arrayexpress

E-GEOD-44793

Project description:Transcription profiling by array of Arabidopsis seedlings grown after DNA-damaging agent bleomycin (BLM) and/or immune inducer 2, 6-dichloroisonicotinic acid (INA) treatment to identify synergistically induced defence genes

2015-04-07 | E-GEOD-44793 | ExpressionAtlas

Transcription profiling of S. cerevisiae sgs1 mutant and wild type strains grown under DNA damaging and control conditions

Project description:Wild type and sgs1 null yeast were grown under DNA damaging (with MMS) conditions or without treatment to log phase and their transcriptional profiles compared. The human aging diseases Werner and Bloom syndromes are a result of mutation of the WRN and BLM genes, respectively. The SGS1 gene of Saccharomyces cerevisiae is homologous to the human WRN and BLM genes of the RecQ DNA helicase family. Deletion of SGS1 results in accelerated yeast aging and a reduction in life span as well as cell cycle arrest. We demonstrate that SGS1 deletion, DNA damage, and stress show similar transcriptional responses in yeast. Our comparative analysis of the genome-wide expression response of SGS1 deletion, stress and DNA damage indicates parallel transcriptional responses to cellular insult and aging in yeast.

2007-07-07 | E-GEOD-423 | biostudies-arrayexpress

Onconase Responsive Genes in Human Mesothelioma Cells: Implications for an RNA Damaging Therapeutic Agent

2009-07-09 | GSE17009 | GEO

DNA damaging agent doxorubicin induces the expression of myotonic dystrophy kinase protein in tumor suppressor p53 dependent manner

Project description:Tumor suppressor p53 plays an integral role in DNA damage-induced apoptosis, which is one of the biological processes to protect against tumor progression. Cell shape is dramatically changed undergoing apoptosis, which is often associated with actomyosin contraction; however, it remains entirely elusive how p53 regulates actomyosin contraction in response to DNA-damaging agent. We here show that p53 controls expression of myotonic dystrophy protein kinase (DMPK), which is known to upregulate actomyosin contraction. To identify a novel p53 regulating gene encoding the modulator of myosin, we performed a DNA microarray and then found that, in response to DNA-damaging agent doxorubicin, DMPK expression was increased in a p53-dependent manner.

2019-09-05 | GSE134201 | GEO

Development and Preclinical Efficacy of Novel Transforming Growth Factor-β1 (TGFβ1) siRNAs for Pulmonary Fibrosis

Project description:Idiopathic pulmonary fibrosis is a chronic devastating disease of unknown etiology. No therapy is currently available. A growing body of evidence supports the role of TGFβ1 as the major player in the pathogenesis of the disease. This study designed novel human- and mouse-specific siRNAs and siRNA/DNA chimeras targeting both human and mouse common sequences and evaluated their inhibitory activity in pulmonary fibrosis induced by bleomycin and lung-specific transgenic expression of human TGFβ1. Selective novel sequences of siRNA and siRNA/DNA chimeras efficiently inhibited pulmonary fibrosis, indicating their applicability as tools for treating fibrotic disease in humans. Total RNA was extracted from lung tissue from mice with bleomycin (BLM)-induced lung fibrosis treated with mouse TGFβ1 siRNAs or vehicle on different days after BLM infusion.

2011-11-22 | E-GEOD-28122 | biostudies-arrayexpress

Chemotherapy With DNA Damaging Agents In Colorectal Cancer

Project description:Chemotherapy Treatment alone with DNA damaging drugs might be as effective as chemotherapy combined with surgery in colorectal cancer (CRC) avoiding surgery complications.

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Transcription profiling of human mesothelioma cells sensitive to onconase - implications for an RNA damaging therapeutic agent

Project description:Onconase represents a new class of RNA-damaging drugs. Mechanistically, Onconase is thought to internalize, where it degrades intracellular RNAs such as tRNA and double-stranded RNA, and thereby suppresses protein synthesis. However, there may be additional or alternative mechanism(s) of action. Microarray analysis was used to compare gene expression profiles in untreated human malignant mesothelioma (MM) cell lines and cells exposed to 5 ug/ml Onconase for 24 h. Experiment Overall Design: In this study, microarray analysis was used to compare gene expression profiles in untreated human malignant mesothelioma (MM) cell lines and cells exposed to 5 ug/ml Onconase for 24 h.

2009-07-19 | E-GEOD-17009 | biostudies-arrayexpress

Lewis Lung Carcinoma (LLC) cells in the lung model express lung-specific genes compared to subcutaneous engraftment cells.

Project description:We revealed that tumor cells in the bleomycin-injured lungs (BLM lungs) are differently influenced by their unique lung fibrogenic microenvironment compared to those in subcutaneous tumor models in vivo or in 2-dimensional monolayer culture in vitro.

2022-07-19 | GSE208373 | GEO

Transcriptome analysis of murine lung epithelial cells and fibroblasts derived from bleomycin-injured alveolospheres

Project description:Pulmonary fibrosis is a devatating lung disease with limited effective treatment. Because fibrotic responses are considered to be regulated by complex cellular and molecular circuits, it is difficult to demonstrate a causal relationship of cells, molecules, and cell-cell interactions with diseases in vivo. To overcome this issue, we established bleomycin (BLM)-stimulated alveolosphere model as an ex vivo culture model. To clarify whether our ex vivo model recapitulates the cellular and molecular response to BLM-induced lung injury, we performed RNA-seq (Bulk RNA-seq) analysis of the BLM-stimulated alveolospheres generated by co-culturing murine lung epithelial cells and lung fibroblasts.

2024-04-01 | GSE200297 | GEO

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