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Expression data from Human Umbilical Vein Endothelial Cells (HUVECs) exposed to WT and V30M transthyretin (TTR)

ABSTRACT: The biological effects of TTR proteins in the vasculature remain unknown. We used microarrays to detail the modulation of gene expression on HUVECs by V30M TTR when compared to cells exposed to WT TTR.

ORGANISM(S): Homo sapiens

PROVIDER: GSE44856 | GEO | 2013/09/13

SECONDARY ACCESSION(S): PRJNA192422

REPOSITORIES: GEO

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Expression data from Human Umbilical Vein Endothelial Cells (HUVECs) exposed to WT and V30M transthyretin (TTR)

Project description:The biological effects of TTR proteins in the vasculature remain unknown. We used microarrays to detail the modulation of gene expression on HUVECs by V30M TTR when compared to cells exposed to WT TTR. HUVECs (passage 7) were cultured in the presence of WT or V30M TTR at 4M-BM-5M for 3 hours. RNA was extracted and hybridized on Affymetrix microarrays. We sought to obtain differentially expressed genes modulated by V30M TTR protein.

2013-09-13 | E-GEOD-44856 | biostudies-arrayexpress

Expression data from Human Umbilical Vein Endothelial Cells

2016-08-11 | GSE85468 | GEO

Expression data from Human Umbilical Vein Endothelial Cells

Project description:Examine the toxic effect and molecular mechanisms of PM2.5 in primary human umbilical vein endothelial cells (HUVECs) . We used microarrays to detail the global programme of gene expression in HUVECs exposed to PM2.5 and identified distinct classes of up-regulated genes. Cultured HUVECs which were treated with PM2.5 at the concentrations of 50 Î¼g/mL or DMEM for 24 h were collected for RNA extraction and hybridization on Affymetrix microarrays.

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The development of microRNA expression profile of human umbilical vein endothelial cells (HUVECs) after exposed to HCCLM3 cells

Project description:we have employed whole microRNA microarray to see the microRNA expression changes of HUVECs after exposed to HCCLM3 cells examine the microRNA expression profile of HUVECs in the absence or presence of human HCC cells

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Project description:we have employed whole microRNA microarray to see the microRNA expression changes of HUVECs after exposed to HCCLM3 cells

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MicroRNA signature of young and replicative senescent human umbilical vein endothelial cells (HUVECs)

Project description:MicroRNAs regulate various cellular processes. While several genes associated with replicative senescence have been described in endothelial cells, miRNAs that regulate these genes remain largely unknown. The present study was designed to identify miRNAs associated with replicative senescence and their target genes in HUVECs. We have employed Agilent Human MicroRNAs microarray platform to evaluate the expressions of 866 human miRNAs and 89 human viral miRNAs, based on Sanger miRNA database release 12.0 miRNA expression profiles were established for young and replicative senescent HUVECs

2013-04-05 | E-GEOD-37092 | biostudies-arrayexpress

Gene signature of young and replicative senescent human umbilical vein endothelial cells (HUVECs)

Project description:MicroRNAs regulate various cellular processes. While several genes associated with replicative senescence have been described in endothelial cells, miRNAs that regulate these genes remain largely unknown. The present study was designed to identify miRNAs associated with replicative senescence and their target genes in HUVECs. Gene profiling was established using the same RNA input as that used for miRNA profiing. We have employed Agilent Whole Human Genome microarray platform to evaluate the expressions of 19,596 human genes . Gene expression profiles were established for young and replicative senescent HUVECs

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DNA methylation profiles of human umbilical vein endothelial cells (HUVECs)

Project description:Replicative senescence has major impact on function and integrity of cell preparations. DNA-methylation (DNAm) changes are continuously acquired at specific CpG dinucleotides during in vitro culture. Such modifications can be used to estimate the state of cellular senescence for quality control of cell preparations. It is however unclear how senescence-associated DNAm changes are regulated and if they occur simultaneously throughout cell populations. In this study, we analyzed global DNAm profiles of human mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVEC) to demonstrate that senescence-associated DNAm changes are overall similar in these different cell types. Subsequently, an Epigenetic-Senescence-Signature, based on six CpGs, was either analyzed by pyrosequencing or by barcoded bisulfite amplicon sequencing. There was a good correlation between predicted and real passage numbers in bulk populations of MSCs (R² = 0.67) and HUVECs (R² = 0.97).

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The genome expression profile of human umbilical vein endothelial cells (HUVECs) overexpressing miR-146a

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2013-04-13 | E-GEOD-46022 | biostudies-arrayexpress

RNA-binding protein RPS27 and gene expression regulation in human umbilical vein endothelial cells (HUVECs)

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2024-03-31 | GSE255600 | GEO

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