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Identification of targets regulated by HSF1 in response to HSP90 inhibitors

ABSTRACT: A375 cells with inducible knockdown HSF1 with and without HSP90 inhibitor treatment

ORGANISM(S): Homo sapiens

PROVIDER: GSE44867 | GEO | 2013/03/06

SECONDARY ACCESSION(S): PRJNA192201

REPOSITORIES: GEO

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Identification of targets regulated by HSF1 in response to HSP90 inhibitors

Project description:A375 cells with inducible knockdown HSF1 with and without HSP90 inhibitor treatment A375 cells were seeded in 6-well plates for 3d with 100ng/ml doxycycline prior to treatment with 0.1% DMSO or 100nM HSP990 for 3h

2013-03-06 | E-GEOD-44867 | biostudies-arrayexpress

Identification of targets regulated by MLL1 in response to HSP90 inhibitors

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Identification of genes regulated by knockdown of HSF1 or RPA1

Project description:Transcription factor access to regulatory elements is prevented by the nucleosome. Heat shock factor 1 (HSF1) is a winged helix transcription factor that plays roles in control and stressed conditions by gaining access to target elements, but mechanisms of HSF1 access have not been well known in mammalian cells. We show a physical interaction between the wing motif of human HSF1 and replication protein A (RPA), which is involved in DNA metabolism. Depletion of RPA1 abolishes HSF1 access to the promoter of HSP70 in unstressed conditions, and delays its rapid activation in response to heat shock. The HSF1-RPA complex leads preloading of RNA polymerase II and opens chromatin structure by recruiting a histone chaperone FACT. Furthermore, this interaction is required for melanoma cell proliferation. These results provide a mechanistic basis for constitutive HSF1 access to nucleosomal DNA, which is important for both basal and inducible gene expression. To examine whether the HSF1-RPA complex regulates the expression of genes, mRNA levels in MEFs with/without HSF1- or RPA1-shRNA were analyzed by DNA microarray analysis using GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix).

2012-12-31 | E-GEOD-38412 | biostudies-arrayexpress

Identification of genes regulated by knockdown of HSF1 or RPA1

2012-12-31 | GSE38412 | GEO

Identification of genes regulated by HSF1 in control and heat-shocked cells

Project description:To examine the regulation of HSF1-mediated gene expression in control and heat shock conditions, we performed DNA microarray analysis. mRNA levels in control and heat-shocked MEF cells, which were infected with adenovirus expressing scramble RNA or HSF1 short hairpin RNA, were analyzed by DNA microarray analysis using GeneChip Mouse Gene 1.0 ST Array (Affymetrix).

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HSP90 inhibitors disrupt a transient HSP90-HSF1 interaction and identify a noncanonical model of HSP90-mediated HSF1 regulation.

Project description: Not available

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Identification of genes regulated by HSF1-T20A mutant or doxorubicin treatment

Project description:To examine whether HSF1-PARP1-PARP13 complex regulates the expression of genes, we performed DNA microarray analysis. We also performed DNA microarray analysis to identify doxorubicin-induced genes,.

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Identification of genes regulated by HSF1 in control and heat-shocked cells

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