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Genome-scale methylation changes induced by photoperiods in Nasonia vitripennis

ABSTRACT: Seasonal photoperiodic changes have strong impact on development in Nasonia vitripennis. Here, Using high-throughput Reduced Representation Bisulfite Sequencing (RRBS) and single-molecule-based sequencing, we generated DNA methylation maps of female wasps maintained in long vs short day. We have identified differential methylated loci that encode the photoperiodic change.

ORGANISM(S): Nasonia vitripennis

PROVIDER: GSE44869 | GEO | 2014/09/01

SECONDARY ACCESSION(S): PRJNA192579

REPOSITORIES: GEO

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Genome-scale methylation changes induced by photoperiods in Nasonia vitripennis

Project description:Seasonal photoperiodic changes have strong impact on development in Nasonia vitripennis. Here, Using high-throughput Reduced Representation Bisulfite Sequencing (RRBS) and single-molecule-based sequencing, we generated DNA methylation maps of female wasps maintained in long vs short day. We have identified differential methylated loci that encode the photoperiodic change. analysis of DNA methylation in female wasps maintained in long vs short day, using RRBS followed by Illumina sequencing

2014-09-01 | E-GEOD-44869 | biostudies-arrayexpress

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Gene expression profile in the reicprocal F1 hybrids of two jewel wasp species Nasonia vitripennis and Nasonia giraulti [RNA-seq]

Project description:We quantified genome-wide total and allele-specific expression in two non-social parasitoids wasp species Nasonia vitripennis and Nasonia giraulti and their reciprocal F1 hybrids. No parent-of-origin effect in allelic expression was found for >8,000 informative genes, suggesting lack of genomic imprinting in adult Nasonia. Gene expression divergence between Nv and Ng could be attributed to both significant cis- and trans- regulatory changes during evolution.

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Profiling of expression level in adult female Nasonia vitripennis

Project description:The expression level in the parasitoid Nasonia vitripennis adult female samples was profiled and compared with the methylation pattern. Methylated and non-methylated genes showed markedly different patterns. The expression level was higher for methylated than non-methylated genes. non-nmethylated genes account for 99% of the genes that were not found to be expressed in the adult female RNA-seq data (FPKM < 0.1). Unlike methylated genes, the expression distribution of the non-methylated genes was bimodal with a lower expressed group and a moderately expressed group of genes, indicating that methylation status is not the only determinant of high expression in adult females. Methylated genes also have lower coefficient of variation (CV) of expression level across five developmental stages, suggesting that they are expressed more evenly across development. Profiling of expression level in adult female Nasonia vitripennis by RNA-seq

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Double-strand GFP RNA injection reveals no off-target effect in Nasonia vitripennis

Project description:The aim of this experiment is to assess the off-target effect of RNAi in Nasonia vitripennis at transcriptome level by comparing the effect of a non-specific RNAi against GFP together with water or not injected controls.

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