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Feeding with miR-184 affects the mRNA expression profile of queen larvae

ABSTRACT: By comparing mRNA profiles of the two groups of bee larvae (treated by miR-184 and water), we found that there were 279 mRNAs which were differently expressed between treated and control larvae (ratio>2 and p<0.01), of which 200 mRNAs were down-regulated in treated larvae, and 79 mRNAs were down-regulated in controls.

ORGANISM(S): Apis mellifera

PROVIDER: GSE44911 | GEO | 2014/01/06

SECONDARY ACCESSION(S): PRJNA192448

REPOSITORIES: GEO

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Feeding with miR-184 affects the mRNA expression profile of queen larvae

Project description:By comparing mRNA profiles of the two groups of bee larvae (treated by miR-184 and water), we found that there were 279 mRNAs which were differently expressed between treated and control larvae (ratio>2 and p<0.01), of which 200 mRNAs were down-regulated in treated larvae, and 79 mRNAs were down-regulated in controls. To further study the biological function and potential pathways of microRNAs with RNAi phenotypes, we measured mRNA profiles of bee larvae fed with miR-184 (treated) and water (control).

2014-01-06 | E-GEOD-44911 | biostudies-arrayexpress

Escherichia coli strain:184/2aE | isolate:184/2aE

Project description:Escherichia coli strain:184/2aE | isolate:184/2aE Genome sequencing

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Project description:Glaesserella sp. 15-184 strain:15-184-2 Genome sequencing

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Substances in the mandibular glands mediate queen manipulation of larval development and colony transition from growth to reproduction in an annual social bumble bee

Project description:Social organization is commonly dynamic with extreme examples in annual eusocial insects ("annual superorganisms"), but the signals and mechanisms regulating social organization remained elusive. In annual bumble bee colonies, larvae with a close contact to a queen do not differentiate into gynes, pupate at an earlier age, and are commonly smaller than siblings that do not contact a queen. We combined detailed observations, proteomics, microRNA transcriptomics, and gland removal surgery, to study the regulation of brood development and division of labor in the model bumble bee Bombus terrestris. We found that regurgitates fed to larvae by queens and workers differ in their protein and microRNA composition. The proteome of the regurgitate overlaps significantly with that of the mandibular (MG) and hypopharyngeal glands (HPG), suggesting that these exocrine glands are the sources of some regurgitate proteins. The MG and HPG proteomes , but not that of the salivary glands, differed between queens and workers, with the caste-specificity preserved for the MG and regurgitate proteomes. Queens subjected to a surgical removal of the MG showed normal behavior and brood care, but failed to manipulate the developmental program of the brood they reared. These findings suggest that substances in the queen MG are fed to larvae and influence their developmental program. As the colony grows, an increasing number of workers feed larvae and by that reduce the effects of the queen substances, such that she can no longer manipulate the development of all larvae, and the colony switches from producing workers (ergonomic phase) to gynes (reproductive phase).

2023-10-05 | PXD039851 | Pride

Prochlorococcus phage MED4-184

Project description:Cyanophage MED4-184 genome sequencing project

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Profiling of gene expression signatures for human corneal epithelial cell after transfection with microRNA-184 or NC.

Project description:Our previous study identified that microRNA-184 as one of the most highly expressed miRNAs in the corneal epithelium. To gain insight into the underlying mechanisms of miR-184 modulation of corneal epithelial cell proliferation and migration, we probed for its gene targets by microarray analysis . HCECs transfected with miR-184 or a NC for 48 h were harvested and a gene microarray evaluated transcriptome expression patterns. Ectopic miR-184 expression resulted in numerous genes undergoing either upregulation or downregulation. Since miRNAs mediate biological functions by impeding expression of their target genes in mammals, a total of 901 differentially downregulated genes (Foldchange ≤ 0.667) were firstly selected.

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MicroRNA profiling of the developing mammary gland identifies miR-184 as a candidate breast tumour suppressor gene (miRNA)

Project description:The study of mammalian development has offered many insights into the molecular aetiology of cancer. We previously used analysis of mammary morphogenesis to discover a critical role for GATA-3 in mammary developmental and carcinogenesis. In recent years an important role for MicroRNAs (miRNAs) in a myriad of cellular processes in development and in oncogenesis has emerged. In this study, microRNA profiling of stromal and epithelial cellular subsets microdissected from the developing mouse mammary gland revealed many microRNAs with expression restricted to various cellular subsets. MicroRNA-184 (miR-184) was exclusively expressed in epithelial cells and markedly upregulated during differentiation of the proliferative, invasive cells of the pubertal terminal end bud (TEB) into ductal epithelial cells in vivo and in FACS-sorted mammary stem cells (MaSCs) versus luminal epithelial cells. miR-184 expression was silenced in mouse tumour models compared to non-transformed epithelium and in a majority of breast cancer cell line models. Ectopic reactivation of miR-184 inhibited the proliferation and self-renewal of metastatic triple negative breast cancer (TNBC) cell lines in vitro and delayed tumour formation and reduced metastasis in vivo. Gene expression studies uncovered multi-factorial direct regulation of genes in the AKT/mTORC1 pathway by miR-184. In clinical breast cancer tissues, expression of miR-184 is lost in primary TNBCs while the miR-184 promoter is methylated in a subset of lymph node metastases from TNBC patients. These studies elucidated a new layer of regulation in the PI3K/AKT/mTOR pathway with relevance to mammary development and tumour proliferation and metastasis.

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