Transcriptomics,Genomics

Dataset Information

25

Ssn6 Overexpression


ABSTRACT: We have performed a genome wide expression profile study of the essential transcriptional co-repressor protein Ssn6 in Schizosaccharomyces pombe. Ssn6 affected targets were identified by over expression of Ssn6 protein in a WT strain. A WT strain holding a plasmid expressing Ssn6 (pDUAL-Ssn6) was compared against the expression in a WT with the empty plasmid (pDUAL-EMPTY) strain. Keywords: Overexpression/WT Overall design: Gene expression profiling experiment in which gene expression in logarithmically growing cultures of cells expressing the Ssn6 plasmid was compared to that in wt controls expressing the empty plasmid. Wt and Ssn6 overexpressing cells were grown in MM-URA media, and RNA was extracted and subjected to cDNA expression profiling analysis using the established protocols (Xue et al, 2004). Two independent RNA preparations were used to hybridise to two microarrays one on which wildtype was labelled with Cy5 and with Cy3 and the other with dye swap. Data normalized with 'Lowess' per chip per spot normalization.

INSTRUMENT(S): Eurogentec S. pombe ORF array (L040B)

SUBMITTER: Anthony Wright  

PROVIDER: GSE4502 | GEO | 2006-11-15

SECONDARY ACCESSION(S): PRJNA104679

REPOSITORIES: GEO

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Publications

Individual subunits of the Ssn6-Tup11/12 corepressor are selectively required for repression of different target genes.

Fagerström-Billai Fredrik F   Durand-Dubief Mikaël M   Ekwall Karl K   Wright Anthony P H AP  

Molecular and cellular biology 20061113 3


The Saccharomyces cerevisiae Ssn6 and Tup1 proteins form a corepressor complex that is recruited to target genes by DNA-bound repressor proteins. Repression occurs via several mechanisms, including interaction with hypoacetylated N termini of histones, recruitment of histone deacetylases (HDACs), and interactions with the RNA polymerase II holoenzyme. The distantly related fission yeast, Schizosaccharomyces pombe, has two partially redundant Tup1-like proteins that are dispensable during normal  ...[more]

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