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Whole genome microarray of T lymphoma invasion and metastasis (Tiam1) silenced retinoblastoma Y79 cells

ABSTRACT: We hypothesized that Tiam1 is involved in invassiveness of retinoblastoma. The fuctional role of Tiam1 in cell progression and metastasis was tested by siRNA mediated knockdown of Tiam1 in retinoblastoma Y79 cells. The genes de-regulated in response to Tiam1 knockdown was analysed by cDNA microarray in which most of the actin cytoskeleton regulation proteins and apoptotic proteins were de-regulated. our results prove that Tiam1 modulates actin cytoskeleton and cell invasion in retinoblastoma.

ORGANISM(S): Homo sapiens

PROVIDER: GSE45130 | GEO | 2013/03/31

SECONDARY ACCESSION(S): PRJNA192943

REPOSITORIES: GEO

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Whole genome microarray of T lymphoma invasion and metastasis (Tiam1) silenced retinoblastoma Y79 cells

Project description:We hypothesized that Tiam1 is involved in invassiveness of retinoblastoma. The fuctional role of Tiam1 in cell progression and metastasis was tested by siRNA mediated knockdown of Tiam1 in retinoblastoma Y79 cells. The genes de-regulated in response to Tiam1 knockdown was analysed by cDNA microarray in which most of the actin cytoskeleton regulation proteins and apoptotic proteins were de-regulated. our results prove that Tiam1 modulates actin cytoskeleton and cell invasion in retinoblastoma. Retinoblastoma Y79 cells were treated with Tiam1 siRNA for 48hrs and cDNA microarray was performed to analyze the genes regulated by Tiam1 silencing compared to untreated Y79 cells. Experiments were performed in triplicates.

2013-03-31 | E-GEOD-45130 | biostudies-arrayexpress

Differential expression of miRNAs in Retinoblastoma cells (Y79) in response to anti-HMGA2 siRNA treatment

Project description:The main objective of the study is to identify the de-regulated miRNAs in association with HMGA2 (oncogene) in the Retinoblastoma tumorus. The present experiment was carried out using Y79 (Retinoblastoma cell line), as the invitro model of Retinoblastoma tumor. The HMGA2 transcripts were silenced using siRNA and the post-silenced RB cells (at the end of 48 hours) were subjected to miRNA profiling using agilent platform.The key miRNAs de-regulated in this experiment has been validated using qRT-PCR and further their role has been evaluated by addition of specific antagomirs in RB cell lines (Y79 and Weri Rb 1).

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Differential expression of miRNAs in Retinoblastoma cells (Y79) in response to anti-HMGA2 siRNA treatment

2013-10-26 | E-GEOD-51696 | biostudies-arrayexpress

Gene expression analysis study in curcumin treated and control Y79 cells

Project description:Gene expression analysis study in curcumin treated (20µM curcumin treated Y79 cells) and control Y79 cells (Suspension Y79 cells). Source were Y79 retinoblastoma cell line from human.

2009-07-11 | GSE17034 | GEO

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Project description:The proteins secreted from Y79 retinoblastoma cell line were analyzed by the GeLC-MS/MS approach.

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Gene expression profiling of retinoblastoma Y79 cell line post EpCAM silencing using siRNA

Project description:Retinoblastoma Y79 cell line was treated with specific siRNA to silence EpCAM gene expression in vitro and RNA was isolated for microarray experiment to study the changes in the whole gene expression profiling in the Y79 cell line. The study identified 465 up-regulated genes (>1.0 fold) and 205 down-regulated genes (<0.5 fold) in response to knockdown of Ep-CAM.

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Y79 retinoblastoma cell line surface proteome

Project description:The proteins on the surface of Y79 retinoblastoma cell line were analyzed by cell surface biotinylation and streptavidin affinity purification.

2024-01-26 | PXD039517 | Pride

Investigation of miRNA expression patterns in Y79 cells

Project description:We aimed to investigate the miRNA expression patterns in Y79 cells, which are from a representative retinoblastoma cell line.

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Gene expression profiling of retinoblastoma Y79 cell line grown with scaffold compared to cells without scaffold

Project description:Retinoblastoma Y79 cell line was grown on 3D scaffolds and compared its gene expression profile with Y79 cells grown without scaffold.

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Investigation of miRNA expression patterns in Y79 cells

Project description:We aimed to investigate the miRNA expression patterns in Y79 cells, which are from a representative retinoblastoma cell line. We prepared 3 independent sets of cell lysates of Y79 cells in normal culture conditions. Total RNA was prepared from each set of cell lysates using Trizol reagent.

2014-05-30 | E-GEOD-58092 | biostudies-arrayexpress

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