Project description:Transcription profiling of E. coli by exposure of VOCs- Bacillus VOCs vs control Experiments of two-conditions with 3 different time points, Exposure of Bacillus VOCs vs Control
Project description:Transcriptional profiling of C. elegans young adult worms cultured on non-pathogenic Bacillus subtilis strain 67 versus age-matched worms cultured on the control lab food E. coli OP50. The goal was to identify genes regulated in response to differences in diet, which potentially confer immunity to later exposures to pathogenic Bacillus thuringiensis DB27.
Project description:Transcriptional profiling of C. elegans young adult worms cultured on non-pathogenic Bacillus strain 67 versus versus age-matched worms cultured on the control lab food E. coli OP50. The goal was to identify genes regulated in response to differences in diet, which might confer immunity to later exposures to pathogenic Bacillus thuringiensis DB27. One-condition experiment. C. elegans young adults, cultured on : Bacillus strain 67 versus E. coli OP50. 4 biological replicates, including 2 dye-swaps.
Project description:The rapid postharvest deterioration of fresh fruits is closely associated with fungal contamination,particularly by Aspergills niger, which severely compromises storage stability and market value.In this study, a fruit endophytic strain, Bacillus velezensis LY G-26, was isolated and shown toproduce volatile organic compounds (VOCs) with strong antifungal activity. VOCs significantlyinhibited 4. niger mycelial growth, spore gemination, and sporulation, and induced severemembrane disruption and mitochondrial damage. HS-SPME-GC-MS/MS identified 2-nonanone,2,S-dimethylpyrazine, and trans-2,3-dimethylacrylic acid as the primary active VOCs, with2-nonanone exhibiting the strongest inhibition. To enable practical application in food systems,LYG-26 was encapsulated into superabsorbent polymer beads, creating a packaging-compatibleslow-release system. When incorporated into sealed storage containers, the beads continuouslyreleased VOCs and significantly reduced A. niger decay in longan, effectively extending theirpostharvest shelf life. These findings provide a safe and environmentally friendly strategy forpostharvest preservation and shelf-life enhancement.
Project description:Transcriptional profiling of Bacillus subtilis str 3610 cells comparing wild-type to cells containing two copies of the gene slrA. wild-type vs slrAx2. Biological replicates 3
Project description:Global transcriptional profiling of Bacillus subtilis cells comparing fur mutant to mutants of the iron-sparing response: fur fsrA double mutant, fur fbpAB triple mutant, fur fbpC double mutant, and fur fbpABC quadruple mutant fur vs fur fsrA (fsrA), fur vs fur fbpAB (AB), fur vs fur fbpC (C), and fur vs fur fbpABC (ABC). Each experiemnt (fur vs mutant) was conducted three times using three independent total RNA preparations (2 independent experiement + 1 independent dye swap).
Project description:Methylation of DNA is one of the common epigenetic signaling tools that cells use to lock the expression of various genes. In this study, we examined the effects of VOCs exposure in humans on methylation using microarray analysis. We recruited participants who had short-term exposure, long-term exposure, or no exposure. We then analyzed changes in methylation in blood samples from these participants. We found that 1178 genes were hypermethylated and 402 genes were hypomethylated compared with the control group in time-dependent manner. Further research is required to determine the time-dependent effects of VOCs on epigenetic regulation of gene expression. Methylation of DNA in human blood samples (IRB #AS 14039) divided into three groups: control (unexposed workers; n = 12), short-term exposure (workers exposed to VOCs for less than 10 years; n = 12), and long-term exposure (workers exposed to VOCs for more than 10 years; n = 12) was experimented by methylation microarray analysis after exposure to VOCs