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Enterococcus faecalis OG1RF control vs. treatment with ampicillin, bacitracin, or cephalothin

ABSTRACT: Transcriptional profiling to investigate the effect of drug treatment on the E. faecalis cells. For microarray analysis, E. faecalis OG1RF was grown in FMC medium supplemented with 10 mM glucose to an optical density at 600 nm (OD600) of 0.3 and the cultures were divided in 7 aliquots. One aliquot was collected by centrifugation and immediately frozen (untreated control cells). The other aliquots were treated for 30 or 60 min with 1.25 X the minimum inhibitory concentration (MIC) of one of the following CW inhibitors: ampicillin (20 μg ml-1), bacitracin (80 μg ml-1), or cephalothin (80 μg ml-1). After an exposure time of 30 or 60 minutes, each of these cultures was also centrifuged and the pellets frozen. RNA was then isolated from each pellet for microarray analysis. This process was repeated 3 additional times, for a total of four replicates of each condition.

ORGANISM(S): Enterococcus faecalis Enterococcus faecalis OG1RF

PROVIDER: GSE45231 | GEO | 2013/03/16

SECONDARY ACCESSION(S): PRJNA193278

REPOSITORIES: GEO

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Enterococcus faecalis OG1RF control vs. treatment with ampicillin, bacitracin, or cephalothin

Project description:Transcriptional profiling to investigate the effect of drug treatment on the E. faecalis cells. For microarray analysis, E. faecalis OG1RF was grown in FMC medium supplemented with 10 mM glucose to an optical density at 600 nm (OD600) of 0.3 and the cultures were divided in 7 aliquots. One aliquot was collected by centrifugation and immediately frozen (untreated control cells). The other aliquots were treated for 30 or 60 min with 1.25 X the minimum inhibitory concentration (MIC) of one of the following CW inhibitors: ampicillin (20 Î¼g ml-1), bacitracin (80 Î¼g ml-1), or cephalothin (80 Î¼g ml-1). After an exposure time of 30 or 60 minutes, each of these cultures was also centrifuged and the pellets frozen. RNA was then isolated from each pellet for microarray analysis. This process was repeated 3 additional times, for a total of four replicates of each condition. RNA was extracted from four replicate samples of each condition of interest (control cells grown to OD600 = 0.3 in FMC medium supplemented with 10mM glucose, then treated with ampicillin, bacitracin, or cephalothin for 30 or 60 minutes) and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from E. faecalis OG1RF cells grown in BHI medium to mid-log.

2013-03-16 | E-GEOD-45231 | biostudies-arrayexpress

Enterococcus faecalis OG1RF control vs. treatment with vancomycin

2013-03-19 | GSE45300 | GEO

Enterococcus faecalis OG1RF control vs. treatment with vancomycin

Project description:Transcriptional profiling to investigate the effect of drug treatment on the E. faecalis cells. For microarray analysis, E. faecalis OG1RF was grown in FMC medium supplemented with 10 mM glucose to an optical density at 600 nm (OD600) of 0.3 and the cultures were divided in 3 aliquots. One aliquot was collected by centrifugation and immediately frozen (untreated control cells). The other aliquots were treated for 30 or 60 min with 1.25 X the minimum inhibitory concentration (MIC) of vancomycin (10 M-NM-<g ml-1). After an exposure time of 30 or 60 minutes, each of these cultures was also centrifuged and the pellets frozen. RNA was then isolated from each pellet for microarray analysis. This process was repeated 3 additional times, for a total of four replicates of each condition. RNA was extracted from four replicate samples of each condition of interest (control cells grown to OD600 = 0.3 in FMC medium supplemented with 10mM glucose, then treated with vancomycin for 30 or 60 minutes) and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from E. faecalis OG1RF cells grown in BHI medium to mid-log.

2013-03-19 | E-GEOD-45300 | biostudies-arrayexpress

Escherichia coli LC-MS/MS treated by tavaborole

Project description:E.coli ATCC 25922 was overnight grown in 10 ml LB and passaged for 3 h with 1:50 dilution in fresh LB. Treated with tavaborole alone (80 μg/ml, labeled ‘A’) or tavaborole plus amikacin (80 μg/ml +40 μg/ml, labeled ‘AK’) for 6 h, cells were washed in PBS and harvested by centrifugation at 4,000 g, 8 min. After liquid nitrogen flash freezing, cells were stored at -80 °C and transported to company for following experiments

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Normal Equine BALF Proteomics - Functional modelling of an equine bronchoalveolar lavage fluid proteome provides experimental confirmation and functional annotation of equine genome sequences

Project description:Bronchoalveolar lavage fluid was collected from three clinically normal horses by infusing 300 ml of warm saline through a 300 cm foley catheter that was passed through the nasal passage into a terminal bronchus of the lung. BALF was retrieved by manual aspiration using a 60 cc syringe, placed immediately on ice, transported to the laboratory, and centrifuged (600xg 10 minutes 4oC). Aliquots of cell free BALF supernatant were frozen at -80C for subsequent proteomic analysis. Proteomic analysis was performed using a composite sample consisting of 100 g of BALF protein from each of three horses. Triplicate aliquots of the composite (75 g of protein each) were subjected to proteomic analysis as described (McCarthy et al. 2006) except that we did not perform differential detergent fractionation and we used one dimensional liquid chromatography (1D LC) nanospray ionization and not electrospray ionization.

2012-05-27 | PRD000303 | Pride

RNA-seq of human HepG2 cells treated with sodium oleate, 17β-estradiol and ER-alpha siRNA

Project description:HepG2 cells are only treated with 60 μg/ml sodium oleate as group 1, and treated with 60 μg/ml sodium oleate and 50 ng/ml E2 as group 2, and treated with 60 μg/ml sodium oleate, 50 ng/ml E2 and ER-alpha as group 3. All the treatment last for 48 hours and each group have two replications.

2019-10-05 | E-MTAB-7318 | biostudies-arrayexpress

Expression data from APC 50% or 80% -treated HFDPCs

Project description:Gene expression profiling reveals a potential role of APC 50% or APC 80% in stimulating hair growth in dermal papilla cells. HFDPCs were human primary cells line, treated with 5 μg/ml APC 50% for 48 h. Microarray gene expression profiling was conducted for three biological replicates HFDPCs were human primary cells line, treated with 5 μg/ml APC 80% for 48 h. Microarray gene expression profiling was conducted for three biological replicates

2020-12-14 | GSE163091 | GEO

slt2 mutant 4 hours exposure to congo red

Project description:S. cerevisiae slt2Δ strain was grown on YEPD. For Congo Red experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 μg/ml. Cells were collected at 4 hours of growth, frozen at -80 °C and processed for RNA extraction. Keywords: repeat sample

2004-02-16 | GSE964 | GEO

wild type 6 hours exposure to congo red

Project description:S. cerevisiae was grown on YEPD. For Congo Red experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 μg/ml. Cells were collected at 6 hours of growth, frozen at -80 °C and processed for RNA extraction. Keywords: repeat sample

2004-02-16 | GSE963 | GEO

wild type 4 hours exposure to congo red

Project description:S. cerevisiae was grown on YEPD. For Congo Red experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 μg/ml. Cells were collected at 4 hours of growth, frozen at -80 °C and processed for RNA extraction. Keywords: repeat sample

2004-02-16 | GSE962 | GEO

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