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Gene expression Data from Toxoplasma gondii-Infected Murine Bone Marrow-Derived STAT6 and STAT3 Deficient Macrophages

ABSTRACT: The Toxoplasma type I ROP16 kinase directly activates the host STAT3 and STAT6 transcription factors and regulates the expression of many host genes. However, many of genes lack known STAT3/6 transcription factor binding sites in their promoter regions. We wanted to understand what fraction of host genes that are modulated by ROP16 were dependent on the STAT3 and STAT6 signaling pathways.

ORGANISM(S): Mus musculus

PROVIDER: GSE45309 | GEO | 2013/03/20

SECONDARY ACCESSION(S): PRJNA193443

REPOSITORIES: GEO

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Gene expression Data from Toxoplasma gondii-Infected Murine Bone Marrow-Derived STAT6 and STAT3 Deficient Macrophages

Project description:The Toxoplasma type I ROP16 kinase directly activates the host STAT3 and STAT6 transcription factors and regulates the expression of many host genes. However, many of genes lack known STAT3/6 transcription factor binding sites in their promoter regions. We wanted to understand what fraction of host genes that are modulated by ROP16 were dependent on the STAT3 and STAT6 signaling pathways. Bone marrow-derived macrophages (BMMs) from mLys-Mcre Stat3fl/fl mice were infected with type II (Pru A7), type II +ROP16 I, type II Δgra15, or type II Δgra15 + ROP16I and gene expression was analyzed 18 hrs after infection. This data set was generated side by side with infections performed in B6 BMDMs, the gene expression results for that experiment were previoiusly submitted under the GEO accession number GSE29404. In addition, gene expression of wild type B6 and Stat6-/- BMDMs were infected with the II+ROP16I strain.

2013-03-20 | E-GEOD-45309 | biostudies-arrayexpress

The Toxoplasma gondii virulence factor ROP16 acts in cis and trans and suppresses T cell responses [CEP_invivo_Ai6_1dpi]

Project description:The ability of Toxoplasma gondii to inject the rhoptry kinase ROP16 into host cells results in the activation of the transcription factors STAT3 and STAT6, but it is unclear how these events impact infection. Here, parasites that inject Cre-recombinase with rhoptry proteins were used to distinguish infected macrophages from those only injected with parasite proteins. Transcriptional profiling revealed that injection of rhoptry proteins alone was sufficient to induce an M2 phenotype that is dependent on STAT3 and STAT6, but only infected cells displayed reduced expression of anti-microbial genes and antigen presentation pathways. In vivo, the absence of STAT3 or STAT6 improved parasite control while the loss of ROP16 resulted in a marked reduction in parasite numbers and heightened parasite-specific T cell responses. Thus, ROP16 is a virulence factor that can act in cis and trans to promote M2 programs and which limits the magnitude of parasite-specific T cell responses.

2019-11-22 | GSE140773 | GEO

The Toxoplasma gondii virulence factor ROP16 acts in cis and trans and suppresses T cell responses [BMMC_invitro_stim_24hr]

2019-11-22 | GSE140772 | GEO

Expression Data from Toxoplasma gondii-Infected Murine Bone Marrow-Derived Macrophages

Project description:We wanted to determine how type II Toxoplasma GRA15 and type I ROP16 affect host gene expression. We infected bone marrow-derived macrophages (BMMs) from B6 mice with type II (Pru), type II +ROP16 I, type II Δgra15, or type II Δgra15 + ROP16I.

2011-05-20 | GSE29404 | GEO

Expression Data from Toxoplasma gondii-Infected Murine Bone Marrow-Derived Macrophages

2011-05-20 | E-GEOD-29404 | biostudies-arrayexpress

Gene expression profiles of the small intestine and Peyer's patches from mice that were orally infected with Toxoplasma

Project description:The Toxoplasma type I ROP16 kinase directly activates the host STAT3 and STAT6 transcription factors and when transgenically expressed in the orally virulent type II strain, promotes host resistance to oral challenge. The transcriptional profile of type II and II+ROP16I infected Peyer's patches and intestines from orally infected mice on day 5 was determined to elucidate host signaling pathways and molecular gene targets that correlate with protective immunity in the gut of orally challenged animals

2013-03-20 | GSE45310 | GEO

Expression Data from Toxoplasma gondii Infected Murine Bone Marrow-Derived Macrophages

Project description:We wanted to determine how type I ROP16 affect host gene expression We infected BMDM's from BALB/c with type I (RH) and type I (RH) ΔROP16

2011-05-29 | GSE29582 | GEO

Expression Data from Toxoplasma gondii Infected Murine Bone Marrow-Derived Macrophages

Project description:We wanted to determine how type I ROP16 affect host gene expression We infected BMDM's from BALB/c with type I (RH) and type I (RH) ?ROP16 BMDM's were plated, then infected with parasites for 18 hours. Then the RNA was harvested from the cells by Trizol.

2011-05-29 | E-GEOD-29582 | biostudies-arrayexpress

Expression data from RAW264.7 murine macrophages infected with Toxoplasma gondii and subsequently stimulated with interferon-gamma

Project description:Toxoplasma strains are known to inhibit the expression of several interferon-gamma induced genes, and a type II strain was shown to dysregulate genome-wide responses to interferon-gamma in human fibroblasts (Kim et al., 2007, J Immunol.). In this study we aimed to determine the effect of infection with three clonal lineages of Toxoplasma, type I, II, and III strains on genome-wide interferon-gamma induced transcription in murine macrophages. We also assessed the effect of the two main Toxoplasma modulators of mouse macrophage transcription, ROP16 and GRA15 (Jensen et al., 2011, Cell Host Microbe). We used Affymetrix microarrays to analyze host cell transcription after Toxoplasma infection and interferon-gamma stimulation. RAW264.7 murine macrophages were left uninfected or infected with type I (RH), type I ?rop16 (RH ?rop16), type II (Pru), type II ?gra15 (Pru ?gra15), or type II (CEP) parasites at an MOI ~5 for 18 hours and subsequently stimulated with murine IFN-? for six hours. Plaque assays were done to assess parasite viability. Total RNA was isolated and hybridized to Affymetrix Mouse 430A 2.0 gene chips.

2013-02-19 | E-GEOD-34913 | biostudies-arrayexpress

Expression data from RAW264.7 murine macrophages infected with Toxoplasma gondii and subsequently stimulated with interferon-gamma

2013-02-19 | GSE34913 | GEO

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