Transcriptomics,Genomics

Dataset Information

27

MiRNA expression of canine mammary cancer stem cells


ABSTRACT: The aim of our study was to assess miRNA expression of canine mammary cancer stem cells. Three canine mammary cancer cell lines (CMT-U27, CMT-U309 and P114) were stained using Anti-Sca1 (Stem cell antigen 1) antibodies. The FACS analysis showed 0,3-1% of Sca1+ cells in each of the cell line. The cells were sorted (using FACS Aria II) as Sca1+ and Sca1- and subjected to further analysis of miRNA expression (using Agilent custom miRNA microarray). Overall design: Canine mammary cancer cell lines were stained using Stem Cell Antigen 1 antibodies and then sorted using FACS Aria II as Sca-1 positive (cancer stem cells) and Sca-1 negative (control). Then, total RNA was isolated and hybridized at miRNA microarrays.

INSTRUMENT(S): Agilent-037303 Canis familiaris miRNA array (miRNA version)

SUBMITTER: Magdalena Król  

PROVIDER: GSE45622 | GEO | 2014-12-31

SECONDARY ACCESSION(S): PRJNA195433

REPOSITORIES: GEO

Similar Datasets

2016-06-01 | E-GEOD-73230 | ArrayExpress
2007-09-01 | E-GEOD-7659 | ArrayExpress
| PRJNA195433 | ENA
2013-11-11 | E-MTAB-630 | ArrayExpress
2014-09-07 | E-GEOD-54535 | ArrayExpress
| GSE109999 | GEO
2011-12-10 | GSE25586 | GEO
2011-12-10 | E-GEOD-25586 | ArrayExpress
2012-05-30 | E-GEOD-29601 | ArrayExpress
| GSE43968 | GEO