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Anti-BRAF mutation drug resistance enhances EGFR expression in melanomas [methylation profiling]

ABSTRACT: We treated melanoma cells with BRAF mutation with BRAF inhibitor and screened for BRAF inhibitor resistant cells. We extracted DNA from parental cells and resistant cell lines. We compared the DNA methylation via Illumina 450K Methylation Array

ORGANISM(S): Homo sapiens

PROVIDER: GSE45647 | GEO | 2014/12/17

SECONDARY ACCESSION(S): PRJNA195562

REPOSITORIES: GEO

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Anti-BRAF mutation drug resistance enhances EGFR expression in melanomas [methylation profiling]

Project description:We treated melanoma cells with BRAF mutation with BRAF inhibitor and screened for BRAF inhibitor resistant cells. We extracted DNA from parental cells and resistant cell lines. We compared the DNA methylation via Illumina 450K Methylation Array Bisulphite converted DNA from the 4 specimens was hybridised to the Illumina Infinium 450k Human Methylation Beadchip

2014-12-17 | E-GEOD-45647 | biostudies-arrayexpress

Anti-BRAF mutation drug resistance enhances EGFR expression in melanomas [expression profiling]

Project description:We treated melanoma cells with BRAF mutation with BRAF inhibitor and screened for BRAF inhibitor resistant cells. We extracted total mRNA from parental cells and resistant cell lines. We compared their expression by carried out Affymetrix Huex 1.0 ST expression array. Two paired parental/derived cell lines were screened as per the summary above. BRAF inhibitory resistant cells had gene expression compared to the parental cell lines.

2014-12-17 | E-GEOD-45648 | biostudies-arrayexpress

Anti-BRAF mutation drug resistance enhances EGFR expression in melanomas [expression profiling]

2014-12-17 | GSE45648 | GEO

Anti-BRAF mutation drug resistance enhances EGFR expression in melanomas

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-12-17 | E-GEOD-45649 | biostudies-arrayexpress

Expression data from BRAF-mutant melanoma cell lines

Project description:Melanoma cell lines were assessed for differences in gene expression patterns between the lines sensitive and resistant to BRAF and MEK inhibitor drugs. 22 BRAF-mutant melanoma cell lines were assessed for response to BRAF and MEK inhibitors in a 3 day drug treatment dose response assay. Based on the IC50, 18 lines were found to be responsive to BRAF or MEK inhibition and 4 were resistant. Normalised gene expression data generated from experimental replicate affymetrix arrays was assessed to identify differential patterns of inherent gene expression between the cell lines grouped as drug-responsive or drug-resistant. This were used to idenify specific candidate genes and pathways associated with inherent BRAF/MEK inhibitor drug resistance in melanoma cells.

2015-08-26 | E-GEOD-45558 | biostudies-arrayexpress

Anti-BRAF mutation drug resistance enhances EGFR expression in melanomas [expression profiling]

Project description: Not available

| S-ECPF-GEOD-45648 | biostudies-other

Transcriptomic profiling of melanomas treated with BRAF inhibitor

Project description:Transcriptomic profiling of melanomas treated with BRAF inhibitor

| PRJNA678300 | ENA

Anti-BRAF mutation drug resistance enhances EGFR expression in melanomas

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-12-17 | GSE45649 | GEO

Expression of BRAF inhibitor resistant colon cancer lines

Project description:Colon cancer cell lines with partial sensitivity to the BRAF inhibitor PLX4720 were grown in increasing concentration of the drug to develop acquired resistance. Gene expression was performed for comparison of the resistant clones to the parental lines. Colon cancer cell lines with partial sensitivity to the BRAF inhibitor PLX4720 were grown in increasing concentration of the drug to develop acquired resistance. Gene expression was performed for comparison of the resistant clones to the parental lines.

2012-09-15 | E-GEOD-34299 | biostudies-arrayexpress

3' RNAseq of EGFR mutant lung cancer cell line overexpressing BRAF V600E treated with inhibitors

Project description:In the human EGFR mutant adenocarcinoma cell line PC9 pBabe empty vector (EV) or BRAF V600E were overexpressed. RNA was extracted from EV, BRAF V600E overexpressing or osimertinib-resistant BRAF V600E expressing cells after 48h treatment with osimertinib, trametinib, a combination of both or vehicle controls. RNA was subjected to 3' UTR RNA sequencing.

2021-10-20 | E-MTAB-11004 | biostudies-arrayexpress

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