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Changes in PBMC gene expression profiles after influenza vaccination in healthy human subjects

ABSTRACT: Daily sampling of peripheral blood from human subjects vaccinated for influenza was done immediately before vaccination and for 10 days after vaccination. Temporal patterns of gene expression, determined by RNA-seq, in unfractionated PBMC suggested migration of myeloid/dendritic cell lineage cells one day after vaccination.

ORGANISM(S): Homo sapiens

PROVIDER: GSE45735 | GEO | 2013/08/07

SECONDARY ACCESSION(S): PRJNA196146

REPOSITORIES: GEO

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Changes in PBMC gene expression profiles after influenza vaccination in healthy human subjects

Project description:Daily sampling of peripheral blood from human subjects vaccinated for influenza was done immediately before vaccination and for 10 days after vaccination. Temporal patterns of gene expression, determined by RNA-seq, in unfractionated PBMC suggested migration of myeloid/dendritic cell lineage cells one day after vaccination. Five subjects, 11 time points per subject (pre-vaccination and daily for 10 days post-vaccination)

2013-08-07 | E-GEOD-45735 | biostudies-arrayexpress

Changes in gene expression profiles of circulating B cells after influenza vaccination in healthy human subjects

Project description:Daily sampling of peripheral blood from human subjects vaccinated for influenza was done immediately before vaccination and for 10 days after vaccination. In B cells, 90% of transcriptomic variation in subjects who received influenza vaccine within the previous three years was explained by a single temporal pattern unique to the individual. A common set of 742 genes was strongly correlated with the migration of differentiating plasma cell subtypes. Five subjects, 11 time points per subject (pre-vaccination and daily for 10 days post-vaccination)

2013-08-07 | E-GEOD-45734 | biostudies-arrayexpress

Changes in gene expression profiles of circulating B cells after influenza vaccination in healthy human subjects

Project description:Daily sampling of peripheral blood from human subjects vaccinated for influenza was done immediately before vaccination and for 10 days after vaccination. In B cells, 90% of transcriptomic variation in subjects who received influenza vaccine within the previous three years was explained by a single temporal pattern unique to the individual. A common set of 742 genes was strongly correlated with the migration of differentiating plasma cell subtypes.

2013-08-07 | GSE45734 | GEO

Influenza Vaccination Primes Human Myeloid Cell Cytokine Secretion and Natural Killer Cell Function

Project description:51 healthy adults were vaccinated with seasonal influenza vaccine and PBMC were collected before and up to 30 days after vaccination and myloid and lymphoid (NK) cell function in response to restimulation with IL-15 alone virus was assessed.

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A Cell-based Systems Biology Assessment of Human Blood to Monitor Immune Responses After Influenza Vaccination

Project description:Systems biology is an approach to comprehensively study complex interactions within a biological system. Most published systems vaccinology studies have utilized whole blood or peripheral blood mononuclear cells (PBMC) to monitor the immune response after vaccination. Because human blood is comprised of multiple hematopoietic cell types, the potential for masking responses of under-represented cell populations is increased when analyzing whole blood or PBMC. To investigate the contribution of individual cell types to the immune response after vaccination, we established a rapid and efficient method to purify human T and B cells, natural killer (NK) cells, myeloid dendritic cells (mDC), monocytes, and neutrophils from fresh venous blood. Purified cells were fractionated and processed in a single day. RNA-Seq and quantitative shotgun proteomics were performed to determine expression profiles for each cell type prior to and after inactivated seasonal influenza vaccination. Our results show that transcriptomic and proteomic profiles generated from purified immune cells differ significantly from PBMC. Differential expression analysis for each immune cell type also shows unique transcriptomic and proteomic expression profiles as well as changing biological networks at early time points after vaccination. This cell type-specific information provides a more comprehensive approach to monitor vaccine responses. PBMC and six purified cell types from two vaccinated donors were isolated prior to (d0) and at days 1, 3, and 7 post-TIV vaccination for RNA-seq analysis

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Transcriptional response to tetanus vaccination in porcine PBMCs

Project description:Three groups of German Landrace piglets were vaccinated with tetanus toxoid. Transcriptome profiles of PBMC were analysed from blood samples taken 0, 2, 4, 8, 24 and 75 hours after a first vaccination and 0, 2, 4, 8, 24 and 75 hours as well as 14 d after a second vaccination on day 14. pair wise comparison of 0 hours to the respective time points after each vaccination

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Transcriptional response to tetanus vaccination in porcine PBMCs

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A Cell-based Systems Biology Assessment of Human Blood to Monitor Immune Responses After Influenza Vaccination

2015-01-08 | PXD001657 | Pride

Infant antibody repertoires during the first two years of influenza vaccination

Project description:Proteomic analysis of serum IgG antibody repertoire against influenza vaccine HAs in infant donors vaccinated with Fluzone 2017-18/18-19. Dataset consists of peak-response (days 14 and 21 post-vaccination) serum IgG samples eluted by affinity chromatography against IAV or IBV hemagglutinin and the flow-throughs.

2022-08-01 | MSV000090057 | MassIVE

Ten day time course of effect of influenza vaccination on gene expression profiles of PBMC and circulating B cells in mice

Project description:Study was to explore the immune and transcriptomic response to seasonal influenza vaccination (TIV) in mice. For more information see Immport study SDY583. 22 total samples, 6 mice per sample: 11 PBMC samples (0, 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 days post-vaccine boost) and 11 B cell samples (0, 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 days post-vaccine boost)

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