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Expression analysis of Edwardsiella ictaluri 93-146 wild type and mutant strains during catfish fry encounter.

ABSTRACT: Edwardsiella ictaluri is the causitive agent of enteric septicemia of catfish, one of the most important diseases impacting US catfish industry. The overall objective of this study is to identify E. ictaluri genes required for host encounter.

ORGANISM(S): Edwardsiella ictaluri 93-146

PROVIDER: GSE46007 | GEO | 2013/12/15

SECONDARY ACCESSION(S): PRJNA196933

REPOSITORIES: GEO

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Expression analysis of Edwardsiella ictaluri 93-146 wild type and mutant strains during catfish fry encounter.

Project description:Edwardsiella ictaluri is the causitive agent of enteric septicemia of catfish, one of the most important diseases impacting US catfish industry. The overall objective of this study is to identify E. ictaluri genes required for host encounter. 4-plex NimbleGen array study using total RNA obtained from wild type and mutant Edwardsiella ictaluri encountered with or without catfish fry. Each treatment had four biological replica and each plex had two probe sets.

2013-12-15 | E-GEOD-46007 | biostudies-arrayexpress

Expression analysis of Edwardsiella ictaluri 93-146 wild type and mutant strains during catfish serum exposure.

2013-12-15 | GSE46011 | GEO

Expression analysis of Edwardsiella ictaluri 93-146 wild type and mutant strains during catfish serum exposure.

Project description:Edwardsiella ictaluri is the causitive agent of enteric septicemia of catfish, one of the most important diseases impacting US catfish industry. The overall objective of this study is to identify E. ictaluri genes required for serum resistance. 4-plex array study using total RNA obtained from wild type and mutant Edwardsiella ictaluri exposed to heat-inactivated catfish serum and normal serum. Each treatment had four biological replica and each plex had two probe sets.

2013-12-15 | E-GEOD-46011 | biostudies-arrayexpress

Expression analysis of channel catfish anterior kidney at 48h post immersion challenge of vaccinated fish (28 days post vaccination with attenuated Edwardsiella ictaluri) with virulent E. ictaluri

2011-10-05 | GSE32588 | GEO

Expression analysis of channel catfish anterior kidney at 48h post immersion vaccination with attenuated Edwardsiella ictaluri

2011-09-10 | GSE32046 | GEO

Expression analysis of channel catfish anterior kidney at 48h post immersion challenge of vaccinated fish (28 days post vaccination with attenuated Edwardsiella ictaluri) with virulent E. ictaluri

Project description:To determine the global gene transcriptional changes in vaccinated channel catfish anterior kidney after immersion challenge with a virulent Edwardsiella ictaluri compared to sham vaccinated catfish control. The attenuated Edwardsiella ictaluri used for vaccination was Aquavac-ESC which was commercially available, the virulent strain of Edwardsiella ictaluri is the AL93-58 strain. A six chip study using total RNA samples including 3 controls (no vaccination, no challenge) and 3 treated groups (challenge after vaccination). Each RNA sample used for microarray was pooled fish anterior kidney samples (5 fish anterior kidney samples were pooled as one). Each chip measrues the expression level of 65,182 genes. For each sequence, 3 probes were selected. Two copies were prepared on arrays.

2011-10-04 | E-GEOD-32588 | biostudies-arrayexpress

Expression analysis of channel catfish anterior kidney at 48h post immersion vaccination with attenuated Edwardsiella ictaluri

Project description:To determine the global gene transcriptional changes in channel catfish anterior kidney after immersion vaccination with attenuated Edwardsiella ictaluri compared to sham vaccinated catfsih control. The attenuated Edwardsiella ictaluri used for vaccination was Aquavac-ESC which was commercially available. A six chip study using total RNA samples including 3 control and 3 vaccinated samples. Each RNA sample used for microarray was poold fish anterior kidney samples (5 fish anterior kidney samples were pooled as one). Each chip measrues the expression level of 65,182 genes. For each sequence, 3 probes were selected. Two copies were prepared on arrays.

2011-09-09 | E-GEOD-32046 | biostudies-arrayexpress

Edwardsiella ictaluri 93-146

Project description:Expression analysis of Edwardsiella ictaluri 93-146 wild type and mutant strains during catfish fry encounter.

| PRJNA196933 | ENA

Edwardsiella ictaluri 93-146

Project description:Expression analysis of Edwardsiella ictaluri 93-146 wild type and mutant strains during catfish serum exposure.

| PRJNA196932 | ENA

Transcriptomic profiling of the livers of blue catfish (Ictalurus furcatus) after infection with Edwardsiella ictaluri

Project description:We have utilized a high-density oligonucleotide microarray for catfish in order to study the transcriptomic responses of blue catfish following infection with E. ictaluri and to identify and develop important immune-related markers for future characterization and genetic mapping. Microarray analysis of the transcriptome profile of the blue catfish liver following infection with the Gram negative bacterium led to the identification of 103 differentially expressed transcripts. Results indicated the strong upregulation of several pathways likely involved in the inflammatory immune response. A multifaceted response to infection was observed, encompassing the complement cascade, iron regulation, inflammatory cell signaling, and antigen processing and presentation. The induction of several components of the MHC class I-related pathway following infection with an intracellular bacterium is reported here for the first time in fish. Taken together, the microarray results add to our understanding of the teleost immune responses and will provide a solid foundation for future functional characterization, genetic mapping, and QTL analysis of immunity-related genes from catfish. Keywords: Disease state analysis

2007-11-22 | GSE6350 | GEO

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