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Investigation of Alterations in Gene Expression in the Retina Induced by Intravitreal Injection of SH-42 and SH-80

ABSTRACT: In this study, we demonstrate that SH-42 and SH-80, novel heat shock protein 90 inhibitors, suppress retinal neovascularization via inhibition of hypoxia-mediated angiogenesis. To figure out any genotoxic effect of these compounds related with heat shock protein 90 inhibition, we performed microarray analyses with retinal tissues treated with SH-42 and SH-80 for 7 days.

ORGANISM(S): Mus musculus

PROVIDER: GSE46057 | GEO | 2013/04/16

SECONDARY ACCESSION(S): PRJNA197139

REPOSITORIES: GEO

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Investigation of Alterations in Gene Expression in the Retina Induced by Intravitreal Injection of SH-42 and SH-80

Project description:In this study, we demonstrate that SH-42 and SH-80, novel heat shock protein 90 inhibitors, suppress retinal neovascularization via inhibition of hypoxia-mediated angiogenesis. To figure out any genotoxic effect of these compounds related with heat shock protein 90 inhibition, we performed microarray analyses with retinal tissues treated with SH-42 and SH-80 for 7 days. We intravitreally injected PBS, SH42, or SH80 into the right eyes of C57BL/6 male mice (n = 12 per group). PBS-treated mice were regarded as negative control. Four retinal tissues were pooled into 1 test tube and prepared for further analyses.

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