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RIP-Seq analysis of Trypanosoma brucei ELAV-like protein Tb927.8.6650

ABSTRACT: A tagged ectopic version of the ELAV-like protein Tb927.8.6650 of T. brucei was expressed in stable cell lines and pulled down. Co-purifying transcripts were analyzed by sequencing to identify RNAs associated with Tb927.8.6650.

ORGANISM(S): Trypanosoma brucei

PROVIDER: GSE46160 | GEO | 2013/04/18

SECONDARY ACCESSION(S): PRJNA197395

REPOSITORIES: GEO

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RIP-Seq analysis of Trypanosoma brucei ELAV-like protein Tb927.8.6650

Project description:A tagged ectopic version of the ELAV-like protein Tb927.8.6650 of T. brucei was expressed in stable cell lines and pulled down. Co-purifying transcripts were analyzed by sequencing to identify RNAs associated with Tb927.8.6650. Stable procyclic form cell lines expressing tetracycline-inducible TAP-tagged Tb927.8.6650 were created. Cells were harvested 48h after tet-induction, followed by tandem affinity purification of Tb927.8.6650, extraction of co-purified RNA, and sequencing.

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