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Expression data from C. elegans during L4, L4-lethargus, and Adult

ABSTRACT: To identify genes differentially expressed during the molt, we collected RNA 30-40 minutes after feeding cessation at the start of the fourth larval stage (L4) lethargus. Additional time points for RNA collection were in the mid-L4 stage, approximately four hours prior to lethargus, and in the young adult stage, four hours after lethargus. These samples were interrogated with the Affymetrix C. elegans Genome Array. A total of 1,804 gene transcripts were up regulated, and 1,088 gene transcripts were down regulated, during the L4 lethargus period compared to the L4 and Adult stages (false discovery rate (FDR) < 0.05).

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE46289 | GEO | 2014/04/18

SECONDARY ACCESSION(S): PRJNA198532

REPOSITORIES: GEO

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Expression data from C. elegans during L4, L4-lethargus, and Adult

Project description:To identify genes differentially expressed during the molt, we collected RNA 30-40 minutes after feeding cessation at the start of the fourth larval stage (L4) lethargus. Additional time points for RNA collection were in the mid-L4 stage, approximately four hours prior to lethargus, and in the young adult stage, four hours after lethargus. These samples were interrogated with the Affymetrix C. elegans Genome Array. A total of 1,804 gene transcripts were up regulated, and 1,088 gene transcripts were down regulated, during the L4 lethargus period compared to the L4 and Adult stages (false discovery rate (FDR) < 0.05). There were a total of 3 groups and 5x replication for each group, for 15 total samples that were analyzed. The groups were (1) L4, (2) L4-lethargus, (3) Adult. We generated the following pairwise comparisons using R/maanova: L4-lethargus vs L4, L4-lethargus vs Adult. The permutation based p-values for each test were multiplied by 2 and transcripts with an FDRM-bM-^IM-$5% were selected.

2014-04-18 | E-GEOD-46289 | biostudies-arrayexpress

Dirofilaria immitis exhibits sex- and stage-specific differences in excretory/secretory miRNA profiles in vitro

Project description:We report analyses of the types, amounts and stage-dependence of microRNAsfound in D. immitis culture media recovered after incubating 800,000 microfilariae for 6 days, 500 third-stage larvae (L3) and 500 fourth-stage larvae (L4) for 7 days, as well as 40 adult females and 40 adult males for 48 hours, all separately.

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The fourth instar in the silk gland of silkworm LC-MSMS

Project description:In the present study, the silk gland proteomes of the fourth instar and fourth molt were analyzed using liquid chromatography−tandem mass spectrometry. In total, 2654 proteins were identified from the silk gland. High abundance of ribosomal proteins and RR-motif chitin-binding proteins were identified during day 2 of the fourth instar (IV-2) larval developmental stage, and the expression of cuticular proteins analogous to peritrophin (CPAP)-motif chitin-binding proteins was higher during the fourth molt (IV-M). In all, nine enzymes were found to be involved in the chitin regeneration pathway in the silk gland. Among them, two chitinase and two chitin deacetylases were identified as CPAP-motif proteins.

2018-06-04 | PXD008677 | Pride

wt L4

Project description:wild type L4 vs mixed stage reference Keywords: other

2003-12-31 | GSE721 | GEO

glp-4 L4

Project description:glp-4 L4 vs mixed stage reference Keywords: other

2003-12-31 | GSE718 | GEO

TP05 late L4

Project description:Timepoint 05 wild type late-L4 larvae vs mixed stage reference Keywords: other

2003-12-31 | GSE730 | GEO

TP04 early L4

Project description:Timepoint 04 wild type early L4 larvae vs mixed stage reference Keywords: other

2003-12-31 | GSE729 | GEO

TP06 late L4/young adult

Project description:Timepoint 06 wild type late L4/young adults vs mixed stage reference Keywords: other

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TP03 late L3/early L4

Project description:Timepoint 03 wild type late-L3/early L4 larvae vs mixed stage reference Keywords: other

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Changes in expression of small non-coding RNAs/microRNAs during aging in C. elegans

Project description:We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Examination of small RNA expression in four timepoints during adulthood of C. elegans hermaphrodites (spe-9 (hc88), cultured at 23dC), Day 0, 5, 8 and 12 post L4-molt.

2009-12-31 | E-GEOD-18634 | biostudies-arrayexpress

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