Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic and Genomic Profiling of Bronchial Epithelial Cells in Never and Current Smokers

ABSTRACT: Comparison of gene and protein expression in the large airway epithelium of never and current smokers. Keywords: gene expression array-based (RNA / in situ oligonucleotide)

ORGANISM(S): Homo sapiens

PROVIDER: GSE4635 | GEO | 2009/04/10

SECONDARY ACCESSION(S): PRJNA95311

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

MicroRNA expression from bronchial epithelial cell samples of current and never smokers.

Project description:MicroRNA expression was assayed from bronchial epithelial cells collected via bronchoscopy from healthy current and never smoker volunteers in order to determine the effect of cigarette smoke exposure on airway epithelial microRNA expression Keywords: Global microRNA expression profiling Bronchial epithelial cells were collected from current and never smokers via bronchoscopy. Low molecular weight RNA ( < 200 nucleotides) was isolated and hybridized to Invitrogen NCode microRNA microarrays to determine which microRNAs detected in bronchial epithelial cells were differentially expressed in the airways of smokers.

2009-01-28 | E-GEOD-14634 | biostudies-arrayexpress

Gene expression from bronchial epithelial cell samples of current and never smokers.

Project description:mRNA expression was assayed from bronchial epithelial cells collected via bronchoscopy from healthy current and never smoker volunteers in order to determine relationships between microRNA and mRNA expression in bronchial epithelial cell samples across current and never smokers and within the same individual. Keywords: Global mRNA expression profiling

2009-01-29 | GSE14633 | GEO

MicroRNA expression from bronchial epithelial cell samples of current and never smokers.

2009-01-29 | GSE14634 | GEO

Transcription profiling of human buccal and nasal epithelium of current and never smokers

Project description:Smoking is the leading cause of lung cancer death, although only a small percentage of smokers develop the disease. Cigarette smoke exposure is known to cause a field of injury in cells throughout the respiratory tract, and while these airway epithelial cells are morphologically normal, they can undergo genetic alterations in response to cigarette smoke exposure. We used microarrays to analyze the gene expression of epithelial cells in the extrathoracic epithelium, specifically nasal and buccal epithelium, to see if these cells underwent similar genetic alterations in response to tobacco exposure as seen in bronchial epithelial cells as has been previously reported. Experiment Overall Design: Buccal and nasal epithelial cell samples were collected from healthy current and never smokers. RNA was isolated from these samples and hybridized to Affymetrix microarrays. Gene expression from never smokers was compared to never smoker gene expression from bronchial epithelium as well as expression data from other tissues to determine commonalities in expression patterns in normal extra- and intra-thoracic samples. In addition, gene expression from smokers and nonsmokers was compared in bronchial, nasal, and buccal epithelium to determine similarities in gene expression in these tissues in response to cigarette smoker exposure.

2008-10-20 | E-GEOD-8987 | biostudies-arrayexpress

Gene expression from bronchial and nasal epithelial cell samples of healthy current and never smokers.

Project description:mRNA expression was assayed from bronchial epithelial cells collected via bronchoscopy and nasal epithelial cells collected by brushing the inferior turbinate from healthy current and never smoker volunteers in order to determine the relationship between smoking-related gene expression changes in bronchial and nasal epithelium within the same individual. Bronchial epithelial cells were collected from current and never smokers via bronchoscopy, and nasal epithelial cells were collected by brushing the inferior turbinate during the same clinic visit. 1ug of RNA was isolated and hybridized to Affymetrix Human Exon 1.0 ST microarrays to obtain mRNA expression. The genome build upon which transcript assignments are based is hg18 (HuEx-1_0-st-v2.na27.hg18.transcript.csv).

2011-03-14 | E-GEOD-16008 | biostudies-arrayexpress

Antioxidant response gene expression in the bronchial airway epithelial cells of smokers at risk for lung cancer

Project description:Prior microarray studies of smokers at high risk for lung cancer have demonstrated that heterogeneity in bronchial airway epithelial cell gene expression response to smoking can serve as an early diagnostic biomarker for lung cancer. This study examines the relationship between gene expression variation and genetic variation in a central molecular pathway (NRF2-mediated antioxidant response) associated with smoking exposure and lung cancer. We assessed global gene expression in histologically normal airway epithelial cells obtained at bronchoscopy from smokers who developed lung cancer (SC, n=20), smokers without lung cancer (SNC, n=24), and never smokers (NS, n=8). Functional enrichment showed that the NRF2-mediated antioxidant response pathway differed significantly among these groups. Keywords: Global mRNA expression profiling 21 total arrays (20 unique patients) run on total RNA obtained from Bronchial Epithelium of Smokers with Lung Cancer 30 total arrays (24 unique patients) run on total RNA obtained from Bronchial Epithelium of Smokers without Lung Cancer 9 total arrays (8 unique patients) run on total RNA obtained from Bronchial Epithelium of Never Smokers

2010-09-30 | E-GEOD-19027 | biostudies-arrayexpress

Expression data from buccal and nasal epithelium of current and never smokers

2008-07-03 | GSE8987 | GEO

Gene expression from bronchial and nasal epithelial cell samples of healthy current and never smokers.

2011-03-14 | GSE16008 | GEO

Antioxidant response gene expression in the bronchial airway epithelial cells of smokers at risk for lung cancer

2010-10-01 | GSE19027 | GEO

Comparative genomic hybridization of human lung adenocarcinoma in never smokers

Project description:CGH array in Lung Adenocarcinoma in Never Smokers

2010-12-31 | E-TABM-926 | biostudies-arrayexpress