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Expression data from maize stems attacked by European corn borer (ECB, Ostrinia nubilalis) larvae

ABSTRACT: ECB larvae remain damaging pests of conventiational maize production. To better understand the physiological and molecular changes occuring in stem tissue during insect attack we explored the biochemical and transcriptional profiles in these tissues. We used microarrays to detail the transcriptional changes associated with ECB attack and considered these in the context of biochemical markers of plant defense and total protein levels.

ORGANISM(S): Zea mays

PROVIDER: GSE46475 | GEO | 2013/06/01

SECONDARY ACCESSION(S): PRJNA200582

REPOSITORIES: GEO

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Expression data from maize stems attacked by European corn borer (ECB, Ostrinia nubilalis) larvae

Project description:ECB larvae remain damaging pests of conventiational maize production. To better understand the physiological and molecular changes occuring in stem tissue during insect attack we explored the biochemical and transcriptional profiles in these tissues. We used microarrays to detail the transcriptional changes associated with ECB attack and considered these in the context of biochemical markers of plant defense and total protein levels. Maize internode tissue was selected from untreated control plants and those which 5th instar ECB larvae had bored into for 48 h for RNA extraction and hybridization on Affymetrix microarrays.

2013-06-01 | E-GEOD-46475 | biostudies-arrayexpress

Expression profile of maize´s stalk challenged with the corn borer Sesamia nonagrioides

2011-10-01 | GSE28244 | GEO

Expression profile of maize´s stalk challenged with the corn borer Sesamia nonagrioides

Project description:Stalk borers are major pests for some of the most important crops in the world, such as maize or rice. Plant defense mechanisms against these herbivores have been poorly investigated. The maize´s stalk responds to insect feeding activating defense genes including hormone biosynthetic-related or proteinase inhibitor transcripts. The most outstanding conclusion is that cells in the maize´s stalk undergo cell wall fortification after corn borer tunneling. We performed a gene expression profiling to identify those genes differentially expressed in maize after infestation with the corn borer S. nonagrioides. Four genetically unrelated maize inbred lines (EP39, EP42, CM151 and PB130) were infested at VT (tasseling) developmental stage with a mass of approximately 40 eggs of S. nonagrioides laid on the sheath of the main ear. Another four biological replicates per genotype were used as control. Samples for RNA extraction were harvested fifteen days after infestation.

2011-09-30 | E-GEOD-28244 | biostudies-arrayexpress