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Expression data from PDE1C knockdown glioblastoma short-term cultures

ABSTRACT: In our lab we detected focal genomic amplification of PDE1C in 90% of short term GBM cultures. Knocking down of PDE1C was associated with compromised capacity opf these cultures to proliferate, migrate and invade. Therefore we carried out affymetric whole genome expression analysis to identify the down stream gene effectors of this function effects.

ORGANISM(S): Homo sapiens

PROVIDER: GSE46538 | GEO | 2013/05/02

SECONDARY ACCESSION(S): PRJNA200899

REPOSITORIES: GEO

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Expression data from PDE1C knockdown glioblastoma short-term cultures

Project description:In our lab we detected focal genomic amplification of PDE1C in 90% of short term GBM cultures. Knocking down of PDE1C was associated with compromised capacity opf these cultures to proliferate, migrate and invade. Therefore we carried out affymetric whole genome expression analysis to identify the down stream gene effectors of this function effects. IN1472 and IN1760 - 2 GBM short term cultures were transfected with siRNA either for PDE1C (siPD) or non-targeting (siNT) and its down gene effectors were studied.

2013-05-02 | E-GEOD-46538 | biostudies-arrayexpress

Gene expression profile of TARDBP in hepatocellular carcinoma cell line (SK-Hep1)

Project description:TARDBP is TARDBP (TDP-43) is a DNA/RNA binding protein and was mutated in human amyotrophic lateral sclerosis (ALS). However, its function in human cancer has not been fully identified, yet.Thus, We carried out microarray to investigate the down-stream target genes of TARDBP after silencing TARDBP in liver cancer cell SK-Hep1. To identify the role of TARDBP in hepatocellular carcinoma cell line, we performed microarray after knocking down TARDBP in hepatocellular carcinoma cell line (3 siLuc, 3 siTARDBP)

2013-04-22 | E-GEOD-37640 | biostudies-arrayexpress

Homo sapiens

Project description:Expression data from PDE1C knockdown glioblastoma short-term cultures

| PRJNA200899 | ENA

CD34 marks angiogenic tip cells in human vascular endothelial cell cultures.

Project description:The functional shift of quiescent endothelial cells into tip cells that migrate and stalk cells that proliferate is a key event during sprouting angiogenesis. We previously showed that the sialomucin CD34 is expressed in a small subset of cultured endothelial cells and that these cells extend filopodia: a hallmark of tip cells in vivo. In the present study, we characterized endothelial cells expressing CD34 in endothelial monolayers in vitro. We found that CD34-positive human umbilical vein endothelial cells show low proliferation activity and increased mRNA expression of all known tip cell markers, as compared to CD34- negative cells. Genome-wide mRNA profiling analysis of CD34-positive endothelial cells demonstrated enrichment for biological functions related to angiogenesis and migration, whereas CD34-negative cells were enriched for functions related to proliferation. In addition, we found an increase or decrease of CD34-positive cells in vitro upon exposure to stimuli that enhance or limit the number of tip cells in vivo, respectively. Our findings suggest cells with virtually all known properties of tip cells are present in vascular endothelial cell cultures and that they can be isolated based on expression of CD34. This novel strategy may open alternative avenues for future studies of molecular processes and functions in tip cells in angiogenesis. Four HUVEC donors were FACS-sorted by CD34 and divided in CD34-positive and CD34-negative fractions.

2012-01-05 | E-GEOD-34850 | biostudies-arrayexpress

CD34 marks angiogenic tip cells in human vascular endothelial cell cultures.

2012-01-05 | GSE34850 | GEO

Hexadecene Cultures

Project description:Enrichment cultures degrading hexadecene

| PRJEB22083 | ENA

Soil sample cultures

Project description:Soil sample cultures Metagenome

| PRJNA484206 | ENA

RNASeq of Calcoco2 in beta and fat cell lines

Project description:Investigation of the function of Calcoco2 by knocking it down in beta and fat cell lines.

| EGAS00001006127 | EGA

db63ef48-eccf-41e6-92fc-4dc7923f9c04 - samples

Project description:Investigation of the function of Calcoco2 by knocking it down in beta and fat cell lines.

| EGAD00001008654 | EGA

Role of FTO in ovarian cancer (RNA-Seq)

Project description:RNA methylation plays an important role fine tuning translation and subsequently regulating cellular responses and cell fate. The fat mass- and obesity-associated protein (FTO) was recognized as an m6A demethylase and described as an oncogenic factor in leukemia and brain tumors. FTO expression levels are suppressed in ovarian tumors and ovarian cancer stem cells (CSCs). FTO induce cyclic AMP activity through targeting PDE4B and PDE1C by down-regulation of m6A levels in the mRNA transcript. In all or findings point to a tumor suppressor function of FTO in high grade serous OC. FTO induce cyclic AMP activity through targeting PDE4B and PDE1C by down-regulation of m6A levels in the mRNA transcript. In all or findings point to a tumor suppressor function of FTO in high grade serous OC

2020-07-01 | GSE130350 | GEO

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