MicroRNA Target Site Identification by Integrating Sequence and Binding Information

ABSTRACT: High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute protein members can pinpoint microRNA target sites within tens of bases, but leaves the identity of the microRNA unresolved. A flexible computational framework that integrates sequence with cross-linking features reliably identifies the microRNA family involved in each binding event, considerably outperforms sequence-only approaches, and quantifies the prevalence of noncanonical binding modes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE46611 | GEO | 2013/05/25

SECONDARY ACCESSION(S): PRJNA201234

REPOSITORIES: GEO