Unknown

Dataset Information

60

Ibf1 and Ibf2 are DNA-binding proteins required for insulator function in Drosophila [ChIP-seq]


ABSTRACT: Here we report on the identification of two previously uncharacterized proteins as CP190 interacting proteins, that we have named Ibf1 and Ibf2. These proteins localize at insulator bodies and associate with chromatin at CP190-binding sites throughout the genome. We also show that Ibf1 and Ibf2 are DNAbinding proteins that form obligated hetero-oligomers that mediate CP190 binding to chromatin. Moreover, Ibf1 and Ibf2 are necessary for insulator activity in enhancerblocking assays. Taken together our data reveal a novel pathway of CP190 recruitment to chromatin that is required for insulator activity. Overall design: ChIP-Seq peak calling of CP190, Ibf1 and Ibf2 against Input sample in Drosophila melanogaster S2 cells

INSTRUMENT(S): Illumina Genome Analyzer IIx (Drosophila melanogaster)

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: M Lluisa Espinas  

PROVIDER: GSE46614 | GEO |

SECONDARY ACCESSION(S): PRJNA201235

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE46614_RAW.tar Raw
filelist.txt Txt
Items per page:
1 - 2 of 2

Similar Datasets

2014-04-18 | E-GEOD-46614 | ArrayExpress
2014-01-01 | S-EPMC3989656 | BioStudies
2019-01-01 | S-EPMC6432716 | BioStudies
2011-01-01 | S-EPMC3190163 | BioStudies
2015-01-01 | S-EPMC4638101 | BioStudies
2014-01-01 | S-EPMC4172637 | BioStudies
2011-01-01 | S-EPMC3165934 | BioStudies
2016-01-01 | S-EPMC4788238 | BioStudies
2019-01-01 | S-EPMC6911044 | BioStudies
1000-01-01 | S-EPMC4132727 | BioStudies