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ShRNAi induced differentiation time courses in mouse embrionic stem cells.

ABSTRACT: We present an integrated approach to identify genetic mechanisms that control self-renewal in mouse embryonic stem (ES) cells. Short hairpin RNA (shRNA) techniques are employed to down regulate a set of gene-products whose expression patterns suggest self-renewal regulatory functions. We focus on transcriptional regulators and identify seven molecules whose shRNA-mediated depletion induces differentiation, including four whose roles in self-renewal had not been demonstrated. We analyze the expression profiles of embryonic stems (ES) cells transduced with individual shRNA vectors, maintained in the presence of LIF. Keywords: time course

ORGANISM(S): Mus musculus

PROVIDER: GSE4679 | GEO | 2006/05/31

SECONDARY ACCESSION(S): PRJNA96785

REPOSITORIES: GEO

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