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Gene Expression Analysis of Postnatal Day 6 Testes Sired by Paternally Exposed Males to Saline or BEP

ABSTRACT: To determine the changes in gene expression of testes from postnatal day 6 pups, a window in development where spermatogonial stem cells is predominant, following paternal exposure to saline or BEP, a chemotherapeutic coktail used to treat testicular cancer

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE46910 | GEO | 2013/05/15

SECONDARY ACCESSION(S): PRJNA203020

REPOSITORIES: GEO

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Gene Expression Analysis of Postnatal Day 6 Testes Sired by Paternally Exposed Males to Saline or BEP

Project description:To determine the changes in gene expression of testes from postnatal day 6 pups, a window in development where spermatogonial stem cells is predominant, following paternal exposure to saline or BEP, a chemotherapeutic coktail used to treat testicular cancer Postnatal day 6 testes (2) of pups sired by males treated with saline (n=5) or BEP (n=5) for a 9 weeks followed by a 9-week recovery period

2013-05-15 | E-GEOD-46910 | biostudies-arrayexpress

Transcription and Imprinting Dynamics in Developing Postnatal Male Germline Stem Cells

2015-11-09 | GSE62355 | GEO

Transcription and Imprinting Dynamics in Developing Postnatal Male Germline Stem Cells

Project description:Paternal imprinting initiates in primordial germ cells (PGCs), and is considered largely completed at birth. The resulting postnatal spermatogonial stem cells (SSCs) thenself-renew and proliferate to populate the testicular niche, with sexual maturation enabling productive gametogenesis. mRNA profiles of neonatal wild type (WT) mice testis were generated by deep sequencing using Illumina HiSeq 2000 Examination of 2 different histone modifications in mouse spermatogonia Please note that ChIPSeq_Kitplus samples are samples isolated with MACS CD117 microbeads from Miltenyi and ChIPSeq_Kitminus are samples that were not positively selected for Kit.

2015-11-09 | E-GEOD-62355 | biostudies-arrayexpress

Gene expression analysis of eight-cell stage rat embryos sired by paternally exposed males to saline or BEP

2014-03-13 | GSE55842 | GEO

Gene expression analysis of eight-cell stage rat embryos sired by paternally exposed males to saline or BEP

Project description:8-cell stage rat embryos were sired by males treated with saline or a chemotherapeutic cocktail, BEP, consisting of Bleomycin, Etoposide, and Cisplatin for 9 weeks. Animals were given an additional 9 weeks of recovery, without any treatment, prior to mating. qPCR gene expression profiling for 84 stem cell transcription factors. Total RNA extracted from 25-35 embryos at the eight-cell stage sired by control or BEP-treated males after a 9-wk recovery period (2 females mated per male, n= 4 males/treatment).

2014-03-13 | E-GEOD-55842 | biostudies-arrayexpress

Transcriptional profiling of testicular biopsies with Sertoli-cell-only and spermatogonial presence

Project description:The aim of the study was to identify in vivo spermatogonial gene expression within the context of their biological niche. Identification of spermatogonial genes was done by t-testing on the replicates of Score 3 (Spermatogonia) and Score 2 (SCO) testicular biopsies. 3 biological replicates with spermatogonial presence in the tubuli seminiferi, 5 biological replicates with Sertoli-cell-only syndrome.

2009-11-12 | E-GEOD-18997 | biostudies-arrayexpress

GDNF-Regulated Gene Expression in Cultures of Rat Spermatogonial Stem Cells

Project description:Expression of GDNF-regulated genes was studied in cultures of self-renewing rat spermatogonial stem cells established from 8-10 day old rat pups maintained in a defined serum free medium. GDNF is the primary regulator of spermatogonial stem cell self renewal in the rat. GDNF regulated genes were identified using microarray profiling rat spermatogonial stem cells in the presence and absence of GDNF.

2009-04-14 | GSE15652 | GEO

Estrogen regulated genes in rat testes and their relationship to recovery of spermatogenesis after irradiation

Project description:Despite numerous observations of effects of estrogens on spermatogenesis, identification of estrogen-regulated genes in the testis is limited. We previously showed in rats, in which irradiation had completely blocked spermatogonial differentiation, that testosterone (T) suppression with GnRH-antagonist and antiandrogen stimulated spermatogenic recovery and addition of estradiol (E2) to this regimen accelerated this recovery. We report here the global changes in testicular cell gene expression induced by the E2 treatment. By minimizing the changes in other hormones and also having concurrent data on the regulation of the genes by those hormones, we were able to dissect the effects of estrogen on gene expression, independent of gonadotropin or T changes. Expression of 20 genes, largely in somatic cells, was up- or down-regulated between 2- and 5-fold by E2. There were also early germ cell genes whose expression increased but this was a result of a small increase in spermatogonial numbers. The striking enrichment of transcripts not corresponding to known genes among the E2-downregulated probes led to the identification of one as micro-RNA miR-34a. We propose that genes whose expression levels are altered in one direction by irradiation and in the opposite direction by both T suppression and E2 treatment are candidates for controlling the block in differentiation. Several genes, including insulin-like 3 (Insl3), satisfied those criteria. If they are indeed involved in the inhibition of spermatogonial differentiation, they may be candidate targets for clinical treatments to enhance recovery of spermatogenesis following gonadotoxic exposures, such as those resulting from cancer therapy. 6 sample replicates of hormonal ablation plus estradiol treatment, compared to previous 14 Sample replicates of sham treatment and 11 Sample replicates of hormonal ablation treatment groups in GSE15243

2011-11-22 | E-GEOD-24672 | biostudies-arrayexpress

Transcriptional profiling of testicular biopsies with Sertoli-cell-only and spermatogonial presence

2009-11-13 | GSE18997 | GEO

Single-cell RNA-sequencing overviews global inflammatory response in in vitro organ cultured testes

Project description:Background: Production of functional sperms from spermatogonial stem cells in vitro is important for understanding of biological mechanisms underlying physiological spermatogenesis and for treatment of male infertility. However, comparing with the in vivo testes, in vitro spermatogenesis is severely lower efficiency. Here we describe abnormalities occurring in early stage of in vitro spermatogenesis at single cell resolution. Results: While spermatogenesis was similarly progressed between in vivo and in vitro-cultured testes, noticeable acute inflammatory response occurred right after in vitro culture of neonatal testes not only in immune cells but also non-immune testicular somatic cells. Inhibitor treatment revealed NLRP3 inflammasome signaling is key to the inflammation occurring in in vitro cultured testes We also found accumulation of damaged/dead germ cells in in vitro cultured testes, which may be due to dysfunction of sertoli cell phagocytosis. Conclusion: Our data revealed tissue-wide sterile inflammation occurring in in vitro cultured testes, in which DAMPs - NLRP3 inflammasome axis may key element. Thus, these abnormal testicular microenvironments may be a cause of low spermatogenesis efficiency of in vitro spermatogenesis.

2022-12-31 | GSE189805 | GEO

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