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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

ABSTRACT: In order to analyze the global changes in gene expression resulting from induction of NetA-Fra signaling, we carried out a microarray experiment comparing Drosophila third instar wing imaginal discs in which Net+Fra had been overexpressed to age matched wild type wing imaginal discs. RNA extracted from both +NetA-Fra overexpression and wildtype third instar imaginal discs were hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 .

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE47113 | GEO | 2013/05/21

SECONDARY ACCESSION(S): PRJNA203644

REPOSITORIES: GEO

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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

Project description:In order to analyze the global changes in gene expression resulting from induction of NetA-Fra signaling, we carried out a microarray experiment comparing Drosophila third instar wing imaginal discs in which Net+Fra had been overexpressed to age matched wild type wing imaginal discs. RNA extracted from both +NetA-Fra overexpression and wildtype third instar imaginal discs were hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 . Heat shock induced GFP-marked clones ectopically expressing NetA+Fra in larvae were generated. Controls for this study included age matched wildtype third instar wing imaginal discs bearing GFP clones which were prepared in the same manner. Total RNA was extracted from dissected +NetA-Fra vs. control third instar wing imaginal discs and hybridized to the Affymetrix GeneChip Drosophila Genome 2.0.

2013-05-21 | E-GEOD-47113 | biostudies-arrayexpress

SmD3 protein interactome in Drosophila melanogaster wing imaginal discs

Project description:Screening for binding partners of the splicing factor SmD3 and changes in interaction upon depletion of the protein Ecdysoneless (Ecd) in the nubbin domain of third-instar larval wing imaginal discs.

2021-09-09 | PXD019761 | Pride

Transcriptomic analysis of third instar larval wing imaginal discs of Drosophila melanogaster corto and uL11 mutants

Project description:We report here the transcriptomic analysis of Drosophila melanogaster wing imaginal discs from third instar female larvae mutant for corto (cortoL1/corto420) The reference line was the w1118 genetic background of the mutant lines.

2022-08-01 | GSE181926 | GEO

Transcriptome analysis of control (y w) and Paip1 mutant third instar larval wing imaginal discs

Project description:We report the genome-wide RNA profiling of third instar larval wing imaginal discs upon Paip1 mutation

2023-07-31 | GSE146531 | GEO

Transcriptomic analysis of third instar larva wing imaginal discs deregulating Cyclin G in Drosophila melanogaster

Project description:We report here the transcriptomic analysis of Drosophila melanogaster wing imaginal discs from third instar female larvae expressing Cyclin G deleted of the PEST domain (the 25 COOH-terminal amin-acids) under the control of the daugterless-Gal4 ubiquitous driver. The negative control was transgenic flies wearing only the daugterless-Gal4 driver.

2017-07-01 | GSE99462 | GEO

Genome-wide binding of Cyclin G in Drosophila melanogaster third instar larva wing imaginal discs.

Project description:We describe here the genome-wide binding sites of Cyclin G in Drosophila melanogaster wing imaginal discs from third instar female larvae. We used a transgenic line expressing a version Cyclin G deleted of the PEST domain (the 25 COOH-terminal amin-acids) and tagged by Myc under the control of the daugterless-Gal4 ubiquitous driver.

2017-07-01 | GSE99461 | GEO

Analysis of H3K9ac in fly imaginal discs [ChIP-seq]

Project description:We have analyzed ChIP-Seq of H3K9ac and H3K27me3 in wing imaginal discs and eye imaginal discs from Drosophila melanogaster.

2015-07-23 | GSE56550 | GEO

D. melanogaster eye-antennal imaginal discs developmental transcriptome

Project description:Transcriptomes of Drosophila melanogaster eye-antennal imaginal discs at three sequential larval stages: late 2nd instar (72h after egg laying (AEL)), mid 3rd instar (96h AEL) and late 3rd instar (120h AEL).

2018-01-08 | GSE94915 | GEO

Single-cell transcriptomic analysis of the scrib mutant wing imaginal discs

Project description:Investigation of intratumor heterogeneity in the scrib¹ mutant wing imaginal discs. Method: Staged scrib¹ wing imaginal discs were dissected and transferred to DPBS. The wing imaginal discs were dissociated in 0.25% Trypsin-EDTA solution at 37 ℃ for 10 min. Cells were then washed in DPBS and passed through 35μm filter before library preparation. Construction of 10x single cell libraries and sequencing on Illumina platform were performed by Novogene.

2019-08-27 | GSE130566 | GEO

Indentification of Dis3L2 targets in Drosophila melanogaster wing imaginal discs

Project description:We investigated the effect on mRNA expression in Drosophila melanogaster wing imaginal discs following the knockdown of the 3'-5' exoribonuclease Dis3L2.

2016-11-16 | E-MTAB-4545 | biostudies-arrayexpress

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