Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome profiling of Pinus radiata branches reveals new insights into compression wood formation in response to gravity stress

ABSTRACT: Compression (CW) and opposite wood (OW) are formed in the uniderside and upperside of conifer branches respectively in response to gravity stress. We investigated genes differentially transcribed between the underside and upside of radiate pine branches using cDNA microarrays with a view to plant gravitropism.

ORGANISM(S): Pinus radiata

PROVIDER: GSE47167 | GEO | 2014/05/30

SECONDARY ACCESSION(S): PRJNA204971

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome profiling of Pinus radiata branches reveals new insights into compression wood formation in response to gravity stress

Project description:Compression (CW) and opposite wood (OW) are formed in the uniderside and upperside of conifer branches respectively in response to gravity stress. We investigated genes differentially transcribed between the underside and upside of radiate pine branches using cDNA microarrays with a view to plant gravitropism. Six trees with well-developed branches were selected from a radiata pine commercial plantation (aged 13 years) located at Bondo, NSW, Australia (35Âº 16' 44.04 S, 148Âº 26' 54.66 E). The largest branch from each tree was further selected for sampling, including three branches sampled in April 2007 (autumn in Bondo) and three sampled in October 2007 (spring). Bark was removed from the base part (about 10 cm in length) of each branch. Developing xylem tissues were scraped from the exposed upperside and underside surface respectively with a sharp chisel. Samples were immediately placed into 50 ml BD FalconTM tubes filled with liquid nitrogen. Gene expression in the underside and upperside of branches was compared using radiata pine cDNA microarrays.

2014-05-30 | E-GEOD-47167 | biostudies-arrayexpress

Identification of candidate genes affecting juvenile wood density in Pinus radiata

Project description:Wood density is a foundamental quality trait for structural timber, bioenergy and pulp industries. We investigated genes differentially transcribed in radiate pine juvneile trees with distinct wood density using cDNA microarrays.

2012-08-03 | GSE32086 | GEO

Defying Gravity: WEEP promotes negative gravitropism in Prunus persica by establishing asymmetric auxin gradients

Project description:Trees with weeping shoot architectures are valued for their beauty and are a resource for understanding how plants regulate posture control. The Prunus persica (peach) weeping phenotype, which has elliptical downward arching branches, is caused by a homozygous mutation in the WEEP gene. Until now, little was known about the function of WEEP protein despite its high conservation throughout Plantae. Here, we present the results of anatomical, biochemical, biomechanical, physiological, and molecular experiments that provide insight into WEEP function. Our data suggest that weeping peach trees do not have defects in branch structure. Rather, transcriptomes from the adaxial (upper) and abaxial (lower) sides of standard and weeping branch shoot tips revealed flipped expression patterns for genes associated with early auxin response, tissue patterning, cell elongation, and tension wood development. This suggests that WEEP promotes polar auxin transport toward the lower side during shoot gravitropic response, leading to cell elongation and tension wood development. In addition, weeping peach trees exhibited steeper root systems and faster lateral root gravitropic response. This suggests that WEEP moderates root gravitropism and is essential to establishing the set-point angle of lateral roots from the gravity vector. Additionally, size-exclusion chromatography indicated that WEEP proteins self-oligomerize, like other proteins with sterile alpha motif domains. Collectively, our results from weeping peach provide new insight into polar auxin transport mechanisms associated with gravitropism and lateral shoot and root orientation.

2023-12-13 | GSE250141 | GEO

Transcription profiling of maize pulvinus after gravity treatment

Project description:Not much is known about the molecular processes involved during gravitropism in monocot plants such as maize. A microarray based study on the expression of genes after a gravity stimulating the maize pulvinus will provide us with valuable information and a better understanding of the underlying molecular processes involved in monocot gravitropism. Objectives for this study included the identification of genes that were regulated at the transcriptional and translation level during gravitropism in the maize pulvinus. This was achieved by microarray analysis of total RNA versus polyribosome associated RNA during a time-course of gravity stimulation of the maize pulvinus. Experiment Overall Design: Six week old maize plants were gravity stimulated by 90Âº reorientation. Upper (slow elongation) and lower (fast elongation) halves of the most gravity competent pulvini were harvested over a time course ranging from 2 minutes up to one hour (2min, 5 min, 15min, 30min, 60min). Pulvini samples from control (vertical, no gravity stimulation) plants were harvested and labeled as left and right. For each time point, total mRNA and polyribosome-associated mRNA were purified and the transcript profiles analyzed using Affymetrix GeneChipÂ® Maize Genome Arrays. The experiment was repeated twice (two growing seasons) and represent two biological repetitions.

2008-10-18 | E-GEOD-8320 | biostudies-arrayexpress

Identification of candidate genes affecting juvenile wood stiffness in Pinus radiata

Project description:Wood stiffness is the most important wood quality trait of forest trees for structural timber production. We investigated genes differentially transcribed in radiate pine trees with distinct wood stiffness using bulked segregant analysis (BSA) and cDNA microarrays. Transcript accumulation in earlywood (EW) and latewood (LW) of high (HS) and low stiffness (LS) trees in two progeny trials was compared.

2011-06-01 | GSE23020 | GEO

Transcriptome analysis of flowering branches building of Citrus plants induced by light drought (LD) condition [RNA-seq]

Project description:To investigate the cooperation of light drought with flower branches building and flower bud formation in Citrus, we set two groups including 75 days light drought (LD) and CK to uncover which target genes relatived to flower bud and branches building have been influenced by LD. We then performed gene expression profiling analysis using data obtained from RNA-seq of two different treatment.

2022-07-26 | GSE202925 | GEO

Pinus radiata

Project description:Transcriptome profiling of Pinus radiata branches reveals new insights into compression wood formation in response to gravity stress

| PRJNA204971 | ENA

The phenylpropanoid pathway is controlled at different branches by a set of R2R3 MYB C2 repressors in grapevine

Project description:A set of grapevine R2R3 MYB repressors negatively regulate the expression of genes involved in different branches of the phenylpropanoid pathway

2014-09-30 | GSE58742 | GEO

Brassica napus cultivar:Inbred material, late flowering, few branches

Project description:Brassica napus cultivar:Inbred material, late flowering, few branches Raw sequence reads

| PRJNA523520 | ENA

Brassica napus cultivar:Inbred material, late flowering, few branches

Project description:Brassica napus cultivar:Inbred material, late flowering, few branches Raw sequence reads

| PRJNA523514 | ENA