Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In silico nano-dissection: defining cell type specificity at transcriptional level in human disease

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE47185 | GEO | 2013/08/06

SECONDARY ACCESSION(S): PRJNA205042

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

In silico nano-dissection: defining cell type specificity at transcriptional level in human disease

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-08-06 | E-GEOD-47185 | biostudies-arrayexpress

In silico nano-dissection: defining cell type specificity at transcriptional level in human disease (tubulointerstitium)

Project description:To identify genes with cell-lineage-specific expression not accessible by experimental micro-dissection, we developed a genome-scale iterative method, in-silico nano-dissection, which leverages high-throughput functional-genomics data from tissue homogenates using a machine-learning framework. This study applied nano-dissection to chronic kidney disease and identified transcripts specific to podocytes, key cells in the glomerular filter responsible for hereditary proteinuric syndromes and acquired CKD. In-silico prediction accuracy exceeded predictions derived from fluorescence-tagged-murine podocytes, identified genes recently implicated in hereditary glomerular disease and predicted genes significantly correlated with kidney function. The nano-dissection method is broadly applicable to define lineage specificity in many functional and disease contexts. We applied a machine-learning framework on high-throughput gene expression data from human kidney biopsy tissue homogenates and predict novel podocyte-specific genes. The prediction was validated by Human Protein Atlas at protein level. Prediction accuracy was compared with predictions derived from experimental approach using fluorescence-tagged-murine podocytes.

2013-08-06 | E-GEOD-47184 | biostudies-arrayexpress

In silico nano-dissection: defining cell type specificity at transcriptional level in human disease (glomeruli)

2013-08-06 | E-GEOD-47183 | biostudies-arrayexpress

In silico nano-dissection: defining cell type specificity at transcriptional level in human disease (tubulointerstitium)

2013-08-06 | GSE47184 | GEO

In silico nano-dissection: defining cell type specificity at transcriptional level in human disease (glomeruli)

2013-08-06 | GSE47183 | GEO