Genomics

Dataset Information

38

Expression data from D3, siSCR, siPRMT1 and siPRMT8 ES cell derived neurons


ABSTRACT: PRMT1 and PRMT8 knockdown D3 embryonic stem cells were generated (siPRMT) or as a control, scrambled sequence was introduced (siSCR). We used microarray to investigate the effect of PRMT1 and PRMT8 in early embryonic neurogenesis. Overall design: PRMT1 and PRMT8 knockdown D3 embryonic stem cells were generated (siPRMT1 or 8) or as a control, non-modified (D3) cells were used. We also used non target control, this case scrambled sequence was introduced (siSCR). Samples were collected at day 16 of differentiation and used for microarray. Three replicates each.

INSTRUMENT(S): [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]

SUBMITTER: Zoltan Simandi  

PROVIDER: GSE47214 | GEO | 2014-12-01

SECONDARY ACCESSION(S): PRJNA205146

REPOSITORIES: GEO

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Publications


Retinoids are morphogens and have been implicated in cell fate commitment of embryonic stem cells (ESCs) to neurons. Their effects are mediated by RAR and RXR nuclear receptors. However, transcriptional cofactors required for cell and gene-specific retinoid signaling are not known. Here we show that protein arginine methyl transferase (PRMT) 1 and 8 have key roles in determining retinoid regulated gene expression and cellular specification in a multistage neuronal differentiation model of murine  ...[more]