Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide expression profiling of Hic-5 and TGFB in WPMY fibroblasts

ABSTRACT: Identifying the effect of the co-regulator Hic-5 (TGFB1I1) and TGFB on the transcriptional profile of WPMY human prostate fibroblast cells with view to further elucidating the broader biological role of Hic-5 and TGFB on fibroblast.

ORGANISM(S): Homo sapiens

PROVIDER: GSE47354 | GEO | 2013/08/26

SECONDARY ACCESSION(S): PRJNA205278

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide expression profiling of Hic-5 and TGFB in WPMY fibroblasts

Project description:Identifying the effect of the co-regulator Hic-5 (TGFB1I1) and TGFB on the transcriptional profile of WPMY human prostate fibroblast cells with view to further elucidating the broader biological role of Hic-5 and TGFB on fibroblast. Knockdown of Hic5 for 48 hours in PShTert-AR cells significantly altered the androgen regulation of approximately 1347 genes. The effect of Hic-5 knockdown was to suppress androgen regulation of approximately 85% of those transcripts, by either reducing androgen mediated repression or activition of gene expression. Isogenic WPMY-1 fibroblasts stably transfected with short-hairpin RNA against Hic-5 or transfected with non-specific negative control short-hairpin RNA, were kept in 5% hormone stripped FBS RPMI for 48 hours and subsequently treated with either ethanol vehicle control or TGFB for 16hr. Total RNA was extracted. Five independent vehicle and five TGFB siSCR and siHic5 samples were hybrydised to Affymetrix Human Gene 1.0 ST array chips.

2013-08-26 | E-GEOD-47354 | biostudies-arrayexpress

Genome-wide expression profiling of Hic-5 knockdown in PshTert-AR human myofibroblasts

2013-08-26 | GSE47203 | GEO

Genome-wide expression profiling of Hic-5 knockdown in PshTert-AR human myofibroblasts

Project description:Identifying the effect of the co-regulator Hic-5 (TGFB1I1) on global androgen receptor transcriptional activity in PShTert-AR prostate fibroblast cells with view to further elucidating the broader biological role of Hic-5 on fibroblast spceific androgen signaling. Knockdown of Hic5 for 48 hours in PShTert-AR cells significantly altered the androgen regulation of approximately 1347 genes. The effect of Hic-5 knockdown was to suppress androgen regulation of approximately 85% of those transcripts, by either reducing androgen mediated repression or activition of gene expression. PShTert-AR cells were transfected with 10nM non-specific control siRNA (NS) or with commercially avaliable Hic-5 specific siRNA (siHic5) for 24hrs. Cells were kept in 5% hormone stripped FBS RPMI for a further 24 hours and subsequently treated with either ethanol vehicle control or 10nM DHT for 16hr. Total RNA was extracted. Nine independent vehicle and 9 DHT siNS and siHic5 samples were pooled into three groups of vechicle and DHT treated siNS and siHic5 samples and hybrydised to Affymetrix Human Gene 1.0 ST array chips.

2013-08-26 | E-GEOD-47203 | biostudies-arrayexpress

Gene expression analysis of ASPN variant overexpressing WPMY-1 cells [human]

Project description:Gene expression analysis of ASPN variant overexpressing WPMY-1 cells

2020-04-13 | GSE131006 | GEO

Homo sapiens

Project description:Genome-wide expression profiling of Hic-5 and TGFB in WPMY fibroblasts

| PRJNA205278 | ENA

Expression data from human BPH1 and WPMY-1 cells treated with PAO.

Project description:Pao extract is an herbal preparation of the bark of an amazonian rain forest tree,Pao Pereira (Geissospermum vellosii),which could inhibit Benign prostatic hyperplasia (BPH).Characterizing the molecular alterations of BPH1 and WPMY-1 cells treated with PAO is important for understanding the molecular mechanisms of PAO inhibiting BPH. We used microarrays to detail the RNA expression.

2020-06-01 | GSE128856 | GEO

RNAi profiling by array of human WPMY-1 cells with PPARbeta/delta knock down and exposure to PPAR agaonists

Project description:Human WPMY-1 cells were depleted for PPARbeta/delta and exposed to the PPAR agonists GW501516.

2010-12-31 | E-MEXP-2756 | biostudies-arrayexpress

Selective enhancement and repression of glucocorticoid receptor signaling by coregulator Hic-5

Project description:The glucocorticoid receptor (GR) recruits many coregulators via the well characterized AF2 interaction surface in the GR ligand binding domain, but LIM domain coregulator Hic-5 binds to the relatively uncharacterized tau2 activation domain in the hinge region of GR. Requirement of Hic-5 for glucocorticoid-regulated gene expression in U2OS osteosarcoma cells was defined by Hic-5 depletion and global gene expression analysis. Hic-5 depletion had selective and dramatic effects, positive and negative, on both activation and repression of GR target genes. For some hormone-induced genes, Hic-5 facilitated recruitment of the Mediator complex and RNA polymerase II. In contrast, many genes were not regulated by hormone until Hic-5 was depleted. On these genes Hic-5 acted at a very early step of the regulatory process, preventing efficient GR binding on enhancers, chromatin remodeling, and thus preventing glucocorticoid-driven transcriptional regulation. Overall, Hic-5 has selective and diverse roles on GR target genes, functioning as coactivator on some genes and corepressor on others, and either facilitating or opposing the glucocorticoid-driven actions of GR. Hic-5 exhibits multiple mechanisms of action, either regulating GR binding to DNA and chromatin remodeling, or facilitating later steps in transcription complex assembly. We investigate the relationship between GR and Hic5 and identify classes of genes that respond differently when cells are induced with hormone and when Hic5 is knocked down We knock down Hic-5 (TGFB1I1) in U2OS cells using siRNA (siHic5_2) along with nonspecific siRNA (shNS) and assay gene expression changes at 4 different time points of hormone treatment. We also include non-infected control (NI) as a second control at each time point.

2014-03-21 | E-GEOD-46448 | biostudies-arrayexpress

Expression data from human BPH1 and WPMY-1 cells treated with Rauwolfia vomitoria (RWF).

Project description:Rauwolfia vomitoria extract is an herbal preparation of the root bark of a plant in the tropical forests of Africa, which could inhibit Benign prostatic hyperplasia (BPH). Characterizing the molecular alterations of BPH1 and WPMY-1 cells treated with RWF is important for understanding the molecular mechanisms of RWF inhibiting BPH. We used microarrays to detail the RNA expression.

2022-05-11 | GSE151643 | GEO

Transcription profiling by array of human WPMY-1 cells treated with GW501516 ((a PPARbetadelta agonist) or TFGBeta2

Project description:Microarray analyses of WPMY-1 cells, either untreated (solvent) or treated with GW501516 (0.3 µM), TGFBeta2 (10 ng/ml), or both ligands for 6 h.

2010-07-21 | E-MEXP-2748 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data