Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The physicochemistry of capped nanosilver predicts it biological activity in brain endothelial (RBEC4) cells

ABSTRACT: This study uses the RBEC4 cell model to examine if nanosilver of different sizes (10nm and 75nm) and cappings (citrate and polyvinylpyrrolidone(PVP)) affect its cellular permeability and genomic response.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE47543 | GEO | 2013/05/31

SECONDARY ACCESSION(S): PRJNA206022

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

The physicochemistry of capped nanosilver predicts it biological activity in brain endothelial (RBEC4) cells

Project description:This study uses the RBEC4 cell model to examine if nanosilver of different sizes (10nm and 75nm) and cappings (citrate and polyvinylpyrrolidone(PVP)) affect its cellular permeability and genomic response. RBEC4 cells (95% confluent) were screeened for changes in gene expression after 18 hour exposure to 1.0 ppm of PVP or citrate coated nanosilver particles at 10 or 75 nanometer sizes. Analysis indicated that the cellular transcription profile alter by nanosilver 10nm+PVP was distinct from it 75nm counterpart and both sizes of citrate-coated particles.

2013-05-31 | E-GEOD-47543 | biostudies-arrayexpress

The Cellular and Genomic Response of Dopaminergic Neurons (N27) to Commericial “Capped” Nanosilver

Project description:Global gene expression indicated that N27 neurons exposed to each nanoAg material (1.0 ppm, 18 hr) responded primarily to PVP coated nanoAg of both sizes with affected pathways largely associated with mitochondrial dysfunction (PVP 75 nm nanoAg) and Nrf-2 mediated oxidative stress (PVP 10 nm nanoAg) pathways. N27 rat dopaminergic neurons were exposed to non-cytoxic (0.5-5.0 ppm) concentrations of nanoAg of different sizes (10 nm, 75 nm) and “cappings” (PVP, citrate).

2013-06-20 | E-GEOD-48087 | biostudies-arrayexpress

The Cellular and Genomic Response of Dopaminergic Neurons (N27) to Commericial “Capped” Nanosilver

2013-06-20 | GSE48087 | GEO

Assessing the exposure to nanosilver and silver nitrate on fathead minnow gill gene expression and mucus production

Project description:Silver exposure is toxic to fish due to disturbances of normal gill function. A proposed toxicity mechanism of silver nanoparticles (AgNP) is derived from the release of silver ions, similar to silver nitrate (AgNO3). However, some datasets support the fact that AgNP can have unique toxic effects that are mediated at the gill. To determine if differences between AgNO3 and AgNP toxicities exist, fathead minnows were exposed to 20 nm PVP- or citrate-coated silver nanoparticles (PVP-AgNP; citrate-AgNP) at the nominal concentration of 200 μg/L or AgNO3 at nominal 6 μg/L for 96 hr. This nominal concentration was applied to approximate the dissolved fraction of Ag in the AgNP suspensions. Mucus production in the water was measured. While mucus production was initially significantly increased in the first 4 h of exposure in all silver treatments compared to control, a decrease in mucus production was observed following 24-96 h of exposure. To determine which genes/pathways are driving this shift in mucus production, gills were dissected and microarray analysis was performed. Hierarchal clustering of differentially expressed genes revealed that all samples distinctly clustered by treatment. There were 109 differentially expressed genes shared among all Ag treatments compared to controls. However, there were 185, 423, and 615 differentially expressed genes unique to AgNO3, PVP-AgNP, and citrate-AgNP, relative to control. While functional analysis indicated several common enriched pathways, such as aryl hydrocarbon receptor signaling, this analysis also indicated some unique pathways between nanosilver and AgNO3. Our results show that AgNO3, PVP-AgNP, and citrate-AgNP exposure affected mucus production in fish gills and also lead to common and unique transcriptional changes.

2015-06-01 | GSE64259 | GEO

Mouse fetal liver cells: treated with nanosilver particles (nAg) vs control.

Project description:To investigate the differently expressed genes upon the nanosilver particle (nAg) exposure.

2016-01-31 | GSE43331 | GEO

Maize microbiome under nanosilver exposure

Project description:Metatranscriptome analysis of maize rhizosphere microbiome under nanosilver exposure

| PRJEB22046 | ENA

Proteomic and morphological effect of nanosilver on soybean under flooding stress

Project description:Flooding stress has a negative impact on the soybean cultivation in early growth stages. In order to understand the effect of nanosilver on the soybean growth under flooding stress, quantitative proteomics technique was used.

2016-12-14 | PXD001579 | Pride

Gene expression data in mouse liver following exposure to silver nanoparticles

Project description:We used microarrays to determine transcptional responses to silver nanoparticles (AgNPs) in the mouse liver following oral ingestion. Liver is a major target organ for AgNP accumulation after oral or intravenous exposure. Given that physicochemical properties of AgNPs such as surface coating may influence transcriptional responses to exposure, we evaluated AgNPs with two different surface coatings such as citrate (cit) and polyvinylpirrolidone (PVP). We identified common and unique mRNA and lncRNA expression profiles for cit-AgNPs and PVP-AgNPs. PVP-AgNPs induced a more robust transctiptional response characterized by a 2-fold higher number of differentially expressed mRNAs and lncRNAs.

2020-08-28 | GSE139560 | GEO