Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Intragenic DNA methylation modulates alternative splicing by recruiting MeCP2 to promote exon recognition

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE47678 | GEO | 2013/08/13

SECONDARY ACCESSION(S): PRJNA207096

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Intragenic DNA methylation modulates alternative splicing by recruiting MeCP2 to promote exon recognition.

Project description: Not available

| S-EPMC3817542 | biostudies-literature

Intragenic DNA methylation modulates alternative splicing by recruiting MeCP2 to promote exon recognition

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-08-13 | E-GEOD-47678 | biostudies-arrayexpress

Intragenic DNA methylation modulates alternative splicing by recruiting MeCP2 to promote exon recognition [ChIP-Seq]

Project description:Although the function of DNA methylation in gene promoter regions is well established in transcriptional repression, the function of the evolutionarily conserved widespread distribution of DNA methylation in gene body regions remains incompletely understood. Here, we show that DNA methylation is enriched in included alternatively spliced exons (ASEs) and inhibiting DNA methylation results in aberrant splicing of ASEs. The methyl-CpG binding protein MeCP2 is enriched in included ASEs, particularly those that are also highly DNA methylated, and inhibition of DNA methylation disrupts specific targeting of MeCP2 to exons. Interestingly, ablation of MeCP2 results in increased nucleosome acetylation and aberrant skipping events of ASEs. We further show that inhibition of histone deacetylases leads to a highly significant overlap of exon skipping events caused by knocking-down MeCP2. Together, our data indicate that intragenic DNA methylation operates in exon definition to modulate alternative splicing and can enhance exon recognition via recruitment of the multifunctional protein MeCP2, which thereby maintains local histone hypoacetylation through its established interaction with HDACs. MeCP2 ChIP-Seq in IMR90 and HCT116 cells

2013-08-13 | E-GEOD-47677 | biostudies-arrayexpress

Intragenic DNA methylation modulates alternative splicing by recruiting MeCP2 to promote exon recognition [RNA-Seq]

2013-08-13 | E-GEOD-47488 | biostudies-arrayexpress

Intragenic DNA methylation modulates alternative splicing by recruiting MeCP2 to promote exon recognition [RNA-Seq]

2013-08-13 | GSE47488 | GEO

Intragenic DNA methylation modulates alternative splicing by recruiting MeCP2 to promote exon recognition [ChIP-Seq]

2013-08-13 | GSE47677 | GEO

Hypoxia-induced changes in intragenic DNA methylation correlate with alternative splicing in breast cancer.

Project description: Not available

| S-EPMC7117958 | biostudies-literature

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Project description: Not available