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Chondrosarcoma cells: sphere vs adherent

ABSTRACT: Transcriptional profiling of human chondrosarcoma sphere vs adherent cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE47823 | GEO | 2015/04/30

SECONDARY ACCESSION(S): PRJNA208017

REPOSITORIES: GEO

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Chondrosarcoma cells: sphere vs adherent

Project description:Transcriptional profiling of human chondrosarcoma sphere vs adherent cells Two different growth conditions, sphere vs adherent

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Homo sapiens

Project description:Chondrosarcoma cells: sphere vs adherent

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Project description:Transcriptional profile of PCSC spheres in SCM-1% KO (stem-like cells) vs adherent cultures in PCSC-Celprogen medium (differentiated-like cells) Two-condition experiment: Sphere vs. Parental/adherent cells. Biological replicates: 2 sphere replicates , 2 adherent replicates. Early passaged tumor cells were expanded in adherent conditions and then plated in sphere forming conditions (SCM-1KO% medium at low density) to isolate sphere growing cells.

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Expression data from glioblastoma derived sphere lines, adherent cell lines and their original glioblastoma tumor

Project description:Glioblastoma (GBM) derived sphere lines and adherent cell lines are an important tool for research in basic and translational neuro-oncology. Documentation of their genetic identity has become a requirement for scientific journals and grant applications to exclude cross-contamination and misidentification that lead to misinterpretation of results. Here, we report expression data for 26 samples including 4 GBM derived sphere lines (4 x 3 replicates), 2 GBM derived sphere lines passaged through intracranial transplantation (2x 1), 2 adherent GBM derived cell lines (2 + 2 x 3 replicates), 4 corresponding glioblastoma tumors and 2 non-tumor brain tissues.

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