Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of Citalopram Exposure on Osteogenesis in Mouse Calvarial Pre-Osteoblasts

ABSTRACT: Recently, serotonin and serotonin reuptake inhibitor (SSRI) drugs have been shown to have an effect on the development and maintenance of bone. However, little is known about its role in craniofacial development. We used microarray as one tool to determine the gene expression profile of intramembranous (calvarial) derived murine pre-osteoblasts after citalopram (SSRI) exposure.

ORGANISM(S): Mus musculus

PROVIDER: GSE48057 | GEO | 2013/06/19

SECONDARY ACCESSION(S): PRJNA208823

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Effects of Citalopram Exposure on Osteogenesis in Mouse Calvarial Pre-Osteoblasts

Project description:Recently, serotonin and serotonin reuptake inhibitor (SSRI) drugs have been shown to have an effect on the development and maintenance of bone. However, little is known about its role in craniofacial development. We used microarray as one tool to determine the gene expression profile of intramembranous (calvarial) derived murine pre-osteoblasts after citalopram (SSRI) exposure. We isolated whole RNA from MC3T3-E1 cells treated with proliferation media or proliferation media with SSRI at a dose of 10^-4 mol./liter for 3 or 7 days in culture. We then used an Affymetrix array and compared expression profiles between control and experimental treatments.

2013-06-19 | E-GEOD-48057 | biostudies-arrayexpress

Effects of Thyroxine Exposure on Osteogenesis in Mouse Calvarial Pre-Osteoblasts

2013-05-31 | GSE47517 | GEO

Effects of Thyroxine Exposure on Osteogenesis in Mouse Calvarial Pre-Osteoblasts

Project description:Thyroid hormone has a positive effect on endochondral bone growth. Few studies have looked at the interaction between thyroid hormone exposures and intramembranous bone derived cells. We used microarray as one tool to determine the gene expression profile of intramembranous (calvarial) derived murine pre-osteoblsts after thyroxine exposure. We isolated whole RNA from MC3T3-E1 cells treated with proliferation media or proliferation media with thyroxine at a dose of 10^-6 mol./liter for 3 or 7 days in culture. We then used an Affymetrix array and compared expression profiles between control and experimental treatments.

2013-05-31 | E-GEOD-47517 | biostudies-arrayexpress

Altered serotonergic circuitry in SSRI-resistant major depressive disorder patient-derived neurons

Project description:The goals of this study are to generate and study serotonergic neurons from MDD patients that are selective serotonin reuptake inhibitor (SSRI) remitters and SSRI-nonremitters (NR).

2022-02-12 | GSE126512 | GEO

Serotonin-induced hyperactivity in SSRI-resistant major depressive disorder patient-derived neurons

Project description:The goals of this study are to generate and study neurons from MDD patients and examine specific aspects of serotonergic neurotransmission in selective serotonin reuptake inhibitor (SSRI) remitters ® and SSRI-nonremitters (NR)

2019-12-04 | GSE125664 | GEO

Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration

Project description:Gene expression profile at single cell level of calvarial bone stromal cells after drill hole lesion injury (1 mm) at post-lesion day 14 (PLD14) from injured calvarial bones and corresponding unijured controls. Adult wild type animals and endothelial-specific Fbxw7 mutants to study Notch gain-of-function were used.

2024-04-11 | GSE262350 | GEO

Screen mRNAs specifically regulated by mTORC1 in calvarial osteoblasts from mice

Project description:To screen mRNAs specifically regulated by mTORC1, a global mRNA expression profile in calvarial osteoblasts (OBs) from mice with or without OB-specific Tsc1 knockout was developed using microarray. Wild type (WT) or OB-specific Tsc1 knockout (KO) mice were sacrificed, with calvarial osteoblasts harvested and subjected to total RNA extraction.

2015-11-07 | E-GEOD-74781 | biostudies-arrayexpress

Screen mRNAs specifically regulated by mTORC1 in calvarial osteoblasts from mice

2015-11-07 | GSE74781 | GEO

Effects of thyroxine exposure on osteogenesis in mouse calvarial pre-osteoblasts.

Project description: Not available

| S-EPMC3720861 | biostudies-literature

Identification of metabolic pathways in Daphnia magna exposed to selective serotonin reuptake inhibitors using transcriptomic, immunocytochemistry and physiological responses.

Project description:Assessing the risks of long-term exposure to low doses of pharmaceuticals is an identified research need, particularly for those that may act as neural disruptors in non-vertebrate species. Selective serotonin reuptake inhibitors (SSRIs) act by blocking the re-uptake of serotonin in the nerve synapses, increasing the effective concentration of serotonin in the intra-synaptic space and therefore stimulating serotoninergic neurons. This effect is used worldwide to treat clinical depression in humans, with the consequence of their widespread release into the environment. SSRIs have been found to alter the reproductive physiology of D. magna and other invertebrates in a biphasic way. Low levels of fluoxetine stimulated offspring production in Daphnia magna and Ceriodaphnia dubia at 36 and 50 Âµg/l, respectively, but higher exposure levels inhibited reproduction in the same species. In this study we explore the hypothesis that SSRI can affect D. magna juvenile developmental rates and offspring production disrupting serotonin activity that is known to regulate carbohydrate and oxidative metabolic pathways. The primary mode of action was tested using transcriptomic analyses of juveniles and adults exposed to SSRIs (fluoxetine 80 ppbs, fluovoxamine 30 ppbs) and serotonin-immunocytochemistry assays of D. magna brains. Reproduction, respirometry and biochemical measurements allowed to related gene and immunological base effects to phenotypic responses.

2013-03-15 | E-GEOD-45053 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data