Allele-specific chromosome conformations induced by a transgene- and locus-dependent imprinting

ABSTRACT: A reporter transgene displayed parental imprinting in mouse embryos when positioned into the Itga6 gene. The strong lacZ pattern of expression scored in embryos inheriting the transgene from a male was not present when transmitted from a female. The transgene exhibited maternal allele-specific DNA hyper-methylation acquired in the germ-line and histone modifications corresponded to profiles described at known imprinted loci. Chromosome conformation analyzes revealed distinct, parent-of-origin interaction domains, with a more compact structure characterizing the maternally inherited repressed allele. The analysis of such transgene insertions with a selective potential to induce imprinting may help understanding the mechanisms identifying particular loci as targets for allele-specific repression.

ORGANISM(S): Mus musculus

PROVIDER: GSE48148 | GEO | 2013/07/03

SECONDARY ACCESSION(S): PRJNA209038

REPOSITORIES: GEO