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Transcriptomic effects of early life and peripubertal dietary vitamin D deficiency on mouse ovaries

ABSTRACT: Vitamin D2 (VD) and the VD3 receptor (VDR) are proposed to have multiple important physiological roles that include but are not limited to glucose homeostasis, bone health, immune competency and more recently maintenance of normal reproductive physiology. The prevalence of VD deficiency in the US is reported to be as high as 60% in some populations. Several recent studies suggest that reproductive-aged women and their offspring are at the greatest risk for VD deficiency. The objective of our study was to determine the effect of early life (intrauterine through preweaning) and peripubertal VD deficiency on female reproductive physiology.

ORGANISM(S): Mus musculus

PROVIDER: GSE48167 | GEO | 2013/06/21

SECONDARY ACCESSION(S): PRJNA209087

REPOSITORIES: GEO

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Transcriptomic effects of early life and peripubertal dietary vitamin D deficiency on mouse ovaries

Project description:Vitamin D2 (VD) and the VD3 receptor (VDR) are proposed to have multiple important physiological roles that include but are not limited to glucose homeostasis, bone health, immune competency and more recently maintenance of normal reproductive physiology. The prevalence of VD deficiency in the US is reported to be as high as 60% in some populations. Several recent studies suggest that reproductive-aged women and their offspring are at the greatest risk for VD deficiency. The objective of our study was to determine the effect of early life (intrauterine through preweaning) and peripubertal VD deficiency on female reproductive physiology. Profile and compare the ovarian transcriptomes of 2-month-old C57BL/6J mice that received a normal (OC samples) or a VD-deficient diet only during the early life (OE samples) or peripubertal (OP samples). Each diet group (OC, OE and OP) was composed of 4 females (biological replicas).

2013-06-21 | E-GEOD-48167 | biostudies-arrayexpress

Transcriptomic effects of early life and peripubertal dietary vitamin D deficiency on mouse pituitary gland

Project description:Vitamin D2 (VD) and the VD3 receptor (VDR) are proposed to have multiple important physiological roles that include but are not limited to glucose homeostasis, bone health, immune competency and more recently maintenance of normal reproductive physiology. The prevalence of VD deficiency in the US is reported to be as high as 60% in some populations. Several recent studies suggest that reproductive-aged women and their offspring are at the greatest risk for VD deficiency. The objective of our study was to determine the effect of early life (intrauterine through preweaning) and peripubertal VD deficiency on female reproductive physiology. Profile and compare the hypophysis transcriptomes of 2-month-old C57BL/6J mice that received a normal (OC samples) or a VD-deficient diet only during the early life (OE samples) or peripubertal (OP samples). Each diet group (OC, OE and OP) was composed of 4 females (biological replicas).

2013-06-21 | E-GEOD-48169 | biostudies-arrayexpress

Transcriptomic effects of early life and peripubertal dietary vitamin D deficiency on mouse pituitary gland

2013-06-21 | GSE48169 | GEO

Differential gene regulation by a synthetic vitamin D receptor ligand and active vitamin D in human cells

Project description:Vitamin D (VD) exerts a wide variety of biological functions including calcemic activity. VD nutritional status is closely associated with the onset and development of chronic diseases. To develop a VD analog with the desired VD activity but without calcemic activity, we screened synthetic VDR antagonists. We identified DLAM-2b as a VDR ligand in a competitive VDR binding assay for 1α,25(OH)2 vitamin D3 (1,25D3), and DLAM-2b showed an antagonistic effect on 1,25D3-induced cell differentiation in HL60 cells. In a luciferase reporter assay in which human VDR was exogenously expressed in cultured COS1 cells, DLAM-2b acted as a transcriptional antagonist. Consistently, DLAM-2b had an antagonistic effect on the 1,25D3-induced expression of a known VD target gene (CYP24A1), and VDR-bound DLAM-2b was recruited to an endogenous VD response element in chromatin in human keratinocytes (HaCaT) and human colon cancer cells (HCT116) endogenously expressing VDR. In an ATAC-seq assay, the effects of 1,25D3 and DLAM-2b on chromatin reorganization were undetectable in HCT116 cells, while the effect of an androgen receptor (AR) antagonist (bicalutamide) was confirmed in prostate cancer cells (LNCaP) expressing endogenous AR. However, whole genome analysis using RNA-seq and ATAC-seq revealed differential gene expression profiles regulated by DLAM-2b versus 1,25D3. The upregulated and downregulated genes only partially overlapped between cells treated with 1,25D3 and those treated with DLAM-2b. Thus, the present findings illustrate a novel VDR ligand with gene regulatory activity differing from that of 1,25D3.

2023-04-28 | GSE230385 | GEO

VD3-VDR axis regulates the homeostasis and function of alveolar macrophage [RNA-Seq]

Project description:Purpose: To understand the regulation of gene expression of alveolar macrophages by VD3-VDR axis

2019-01-08 | GSE124724 | GEO

VD3-VDR axis regulates the homeostasis and function of alveolar macrophage [RNA-Seq LPS]

Project description:Purpose: To understand the regulation of gene expression of alveolar macrophages by VD3-VDR axis

2019-01-08 | GSE124723 | GEO

VD3-VDR axis regulates the homeostasis and function of alveolar macrophage [ChIP-Seq]

Project description:Purpose: To understand the regulation of gene expression of alveolar macrophages by VD3-VDR axis

2019-01-08 | GSE124722 | GEO

Vitamin D3 affects TNFa mediated activation of dendritic cells

Project description:The ability of dendritic cells (DC) to initiate immunity and induce antigen-specific tolerance makes DC ideal targets for pharmacological intervention into immune responses. NF-kB factors are a family of transcriptional regulators important for DC development and function. However, the identity of NF-kB target genes that are central to DC biology is largely unknown. In the present study inhibition of the NF-kB activation by the IkBa super repressor (IkBa-SR) and DNA microarray analysis were used to determine the repertoire of NF-kB responsive genes in DC. A number of immunomodulatory compounds have been suggested to target the NF-kB signalling pathway and/or NF-kB-mediated transcription of pro-inflammatory target genes. 1a,25-dihydroxyvitamin D3 (VD3) exerts its effects through the vitamin D3 receptor (VDR), a member of the nuclear hormone receptor superfamily. Microarray analysis was also selected as an approach of choice for the analysis of the effects of VD3 on the activation of DC, and to survey the involvement of VDR in repression of NF-kB regulated genes. These genes can be potentially useful targets for the development of more specific anti-inflammatory agents for clinical applications. Keywords: drug treatment, TNFa treatment, VD3 treatment DC were generated in vitro from bone marrow cells of VDRmut and VDRwt control mice as described previously (Hieronymus, T., T. C. Gust, R. D. Kirsch, T. Jorgas, G. Blendinger, M. Goncharenko, K. Supplitt, S. Rose-John, A. M. Muller, and M. Zenke. 2005. Progressive and controlled development of mouse dendritic cells from Flt3+CD11b+ progenitors in vitro. J Immunol 174:2552-2562). Immature DC were pre-treated with 1a,25-dihydroxyvitamin D3 (VD3), or left untreated. TNFa was added where appropriate, and cells incubated further for 4 h. Total RNA was amplified, labelled and hybridised to Affymetrix MOE430A arrays. TNFa up- and down-regulated genes, as well as genes affected by VD3 were analysed.

2012-12-31 | E-GEOD-8334 | biostudies-arrayexpress

Peripubertal Bisphenol A Exposure Imparts Detrimental Acute and Aging-Related Changes in Body Composition, Cognition, and H2S Production and Signaling

Project description:Peripubertal endocrine disruption has immediate and lifelong consequences on health, cognition, and lifespan. Disruption comes from dietary, environmental, and pharmaceutical sources. The plasticizer Bisphenol A (BPA) is one such endocrine disrupting chemical (EDC). However, it’s unclear if peripubertal BPA exposure incites long-lasting physiological, neuro-cognitive, and/or longevity-related metabolic impairments. Catabolism of cysteine via transsulfuration enzymes produces hydrogen sulfide (H2S), a redox-modulating gasotransmitter causative to endocrine and metabolic homeostasis and improved cognitive function with age. As thyroid hormone (TH) regulates hepatic H2S production and BPA is a TH receptor antagonist, we hypothesized BPA exposure during peripubertal development impairs metabolic and neuro-cognitive/behavioral endpoints in aged mice, in part, due to altered peripheral H2S production. Results: To test this, male C57BL/6J mice at 5 weeks of age were orally exposed for 5 weeks to 250 ug BPA/kg defined as low dose group (LD BPA), or 250 mg BPA/kg defined as high dose group (HD BPA). Both LD and HD BPA exposure decreased lean mass and increased fat mass. These changes were accompanied by decreased serum total TH. Additionally, LD BPA had an anxiogenic effect while HD BPA caused cognitive deficits. Notably, HD BPA attenuated renal H2S production both acutely and during aging, which correlated with spatial memory deficits. Innovation and Conclusion: These findings provide a potential mechanism of action for the acute and long-term health impacts of BPA-induced peripubertal endocrine disruption and bolster the need for improved monitoring and limitation of adolescent BPA exposure.

2021-05-25 | PXD025746 | Pride

Analysis of the cellular effect of 1α,25-dihydroxyvitamin D3 on lung NCI-H441 cells

Project description:Low serum levels or deficiency of 1α,25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or acute respiratory distress syndrome, although the molecular mechanisms behind this observation are not yet understood. VD3 is known to stimulate lung maturity, alveolar type II cell differentiation and pulmonary surfactant synthesis. This study aims to expand the knowledge by quantitative characterization of NCI-H441 cells upon VD3 treatment at the proteome level.

2023-05-10 | PXD040622 | Pride

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